Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.)
Doubled haploid (DH) technology is a tool used to develop large numbers of inbred lines and increase the rate of genetic gain by shortening the breeding cycles. However, previous attempts to produce DH sunflower plants (<i>Helianthus</i> spp.) have resulted in limited success. In this re...
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MDPI AG
2023-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/13/2430 |
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| author | Hongxia Wang Hongyan Hou Chao-Chien Jan Wun S. Chao |
| author_facet | Hongxia Wang Hongyan Hou Chao-Chien Jan Wun S. Chao |
| author_sort | Hongxia Wang |
| collection | DOAJ |
| description | Doubled haploid (DH) technology is a tool used to develop large numbers of inbred lines and increase the rate of genetic gain by shortening the breeding cycles. However, previous attempts to produce DH sunflower plants (<i>Helianthus</i> spp.) have resulted in limited success. In this research, we applied gamma-induced parthenogenesis to assist the production of DH sunflowers. The objectives of the study included (1) identifying optimal gamma ray doses for inducing DH sunflowers using two cytoplasmic male sterility (CMS) lines as female plants and two male pollinators with recognizable morphological markers, (2) selecting new male pollinators from wild sunflower varieties, and (3) testing the efficacy of the selected male pollinators using emasculated non-male sterile sunflower lines as female plants. In these experiments, pollen grains were irradiated with gamma ray doses ranging from 50 to 200 Gy. The optimal gamma ray dose for pollen grain irradiation and DH plant production was identified to be 100 Gy. In addition, a cultivated (G11/1440) and a wild-type (ANN1811) sunflower line can be used as common male pollinators for their distinctive morphological markers and wide capacity for DH induction by gamma-irradiated pollen grains. |
| first_indexed | 2024-03-11T01:32:37Z |
| format | Article |
| id | doaj.art-c7dda7a340db4f1cb81d5d15190d1e33 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T01:32:37Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-c7dda7a340db4f1cb81d5d15190d1e332023-11-18T17:17:20ZengMDPI AGPlants2223-77472023-06-011213243010.3390/plants12132430Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.)Hongxia Wang0Hongyan Hou1Chao-Chien Jan2Wun S. Chao3USDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, USAMathematics Department, Minnesota State University, Moorhead, MN 56563, USAUSDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, USAUSDA-ARS, Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, USADoubled haploid (DH) technology is a tool used to develop large numbers of inbred lines and increase the rate of genetic gain by shortening the breeding cycles. However, previous attempts to produce DH sunflower plants (<i>Helianthus</i> spp.) have resulted in limited success. In this research, we applied gamma-induced parthenogenesis to assist the production of DH sunflowers. The objectives of the study included (1) identifying optimal gamma ray doses for inducing DH sunflowers using two cytoplasmic male sterility (CMS) lines as female plants and two male pollinators with recognizable morphological markers, (2) selecting new male pollinators from wild sunflower varieties, and (3) testing the efficacy of the selected male pollinators using emasculated non-male sterile sunflower lines as female plants. In these experiments, pollen grains were irradiated with gamma ray doses ranging from 50 to 200 Gy. The optimal gamma ray dose for pollen grain irradiation and DH plant production was identified to be 100 Gy. In addition, a cultivated (G11/1440) and a wild-type (ANN1811) sunflower line can be used as common male pollinators for their distinctive morphological markers and wide capacity for DH induction by gamma-irradiated pollen grains.https://www.mdpi.com/2223-7747/12/13/2430doubled haploid technologygamma-induced parthenogenesissunflower (<i>Helianthus</i> spp.) |
| spellingShingle | Hongxia Wang Hongyan Hou Chao-Chien Jan Wun S. Chao Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) Plants doubled haploid technology gamma-induced parthenogenesis sunflower (<i>Helianthus</i> spp.) |
| title | Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) |
| title_full | Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) |
| title_fullStr | Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) |
| title_full_unstemmed | Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) |
| title_short | Irradiated Pollen-Induced Parthenogenesis for Doubled Haploid Production in Sunflowers (<i>Helianthus</i> spp.) |
| title_sort | irradiated pollen induced parthenogenesis for doubled haploid production in sunflowers i helianthus i spp |
| topic | doubled haploid technology gamma-induced parthenogenesis sunflower (<i>Helianthus</i> spp.) |
| url | https://www.mdpi.com/2223-7747/12/13/2430 |
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