Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo
<i>Sargassum horneri</i> (SH) and <i>Ulva australis</i> (UA) are marine waste resources that cause environmental and economic problems when entering or multiplying the coastal waters of Jeju Island. We analyzed their anti-diabetic efficacy to assess their reusability as funct...
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MDPI AG
2023-09-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/9/473 |
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| author | Young-Hyeon Lee Hye-Ran Kim Min-Ho Yeo Sung-Chun Kim Ho-Bong Hyun Young-Min Ham Yong-Hwan Jung Hye-Sook Kim Kyung-Soo Chang |
| author_facet | Young-Hyeon Lee Hye-Ran Kim Min-Ho Yeo Sung-Chun Kim Ho-Bong Hyun Young-Min Ham Yong-Hwan Jung Hye-Sook Kim Kyung-Soo Chang |
| author_sort | Young-Hyeon Lee |
| collection | DOAJ |
| description | <i>Sargassum horneri</i> (SH) and <i>Ulva australis</i> (UA) are marine waste resources that cause environmental and economic problems when entering or multiplying the coastal waters of Jeju Island. We analyzed their anti-diabetic efficacy to assess their reusability as functional additives. The alpha-glucosidase inhibitory activity of SH and UA extracts was confirmed, and the effect of UA extract was higher than that of SH. After the induction of insulin-resistant HepG2 cells, the effects of the two marine extracts on oxidative stress, intracellular glucose uptake, and glycogen content were compared to the positive control, metformin. Treatment of insulin-resistant HepG2 cells with SH and UA resulted in a concentration-dependent decrease in oxidative stress and increased intracellular glucose uptake and glycogen content. Moreover, SH and UA treatment upregulated the expression of <i>IRS-1</i>, <i>AKT</i>, and <i>GLUT4</i>, which are suppressed in insulin resistance, to a similar degree to metformin, and suppressed the expression of <i>FoxO1</i>, <i>PEPCK</i> involved in gluconeogenesis, and <i>GSK-3β</i> involved in glycogen metabolism. The oral administration of these extracts to rats with streptozotocin-induced diabetes led to a higher weight gain than that in the diabetic group. Insulin resistance and oral glucose tolerance are alleviated by the regulation of blood glucose. Thus, the SH and UA extracts may be used in the development of therapeutic agents or supplements to improve insulin resistance. |
| first_indexed | 2024-03-10T22:55:37Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T22:55:37Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-c7e02ca8c2b54595bf9927a7d1e447812023-11-19T10:05:04ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-09-014597492751210.3390/cimb45090473Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In VivoYoung-Hyeon Lee0Hye-Ran Kim1Min-Ho Yeo2Sung-Chun Kim3Ho-Bong Hyun4Young-Min Ham5Yong-Hwan Jung6Hye-Sook Kim7Kyung-Soo Chang8Department of Clinical Laboratory Science, Catholic University of Pusan, Busan 46252, Republic of KoreaDepartment of Biomedical Laboratory Science, Dong-Eui Institute of Technology, Busan 47230, Republic of KoreaDepartment of Clinical Laboratory Science, Catholic University of Pusan, Busan 46252, Republic of KoreaBiodiversity Research Institute, Jeju Technopark, Jeju 63608, Republic of KoreaBiodiversity Research Institute, Jeju Technopark, Jeju 63608, Republic of KoreaBiodiversity Research Institute, Jeju Technopark, Jeju 63608, Republic of KoreaBiodiversity Research Institute, Jeju Technopark, Jeju 63608, Republic of KoreaDivision of International Infectious Diseases Control, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Kita-Ku, Okayama 700-8530, JapanDepartment of Clinical Laboratory Science, Catholic University of Pusan, Busan 46252, Republic of Korea<i>Sargassum horneri</i> (SH) and <i>Ulva australis</i> (UA) are marine waste resources that cause environmental and economic problems when entering or multiplying the coastal waters of Jeju Island. We analyzed their anti-diabetic efficacy to assess their reusability as functional additives. The alpha-glucosidase inhibitory activity of SH and UA extracts was confirmed, and the effect of UA extract was higher than that of SH. After the induction of insulin-resistant HepG2 cells, the effects of the two marine extracts on oxidative stress, intracellular glucose uptake, and glycogen content were compared to the positive control, metformin. Treatment of insulin-resistant HepG2 cells with SH and UA resulted in a concentration-dependent decrease in oxidative stress and increased intracellular glucose uptake and glycogen content. Moreover, SH and UA treatment upregulated the expression of <i>IRS-1</i>, <i>AKT</i>, and <i>GLUT4</i>, which are suppressed in insulin resistance, to a similar degree to metformin, and suppressed the expression of <i>FoxO1</i>, <i>PEPCK</i> involved in gluconeogenesis, and <i>GSK-3β</i> involved in glycogen metabolism. The oral administration of these extracts to rats with streptozotocin-induced diabetes led to a higher weight gain than that in the diabetic group. Insulin resistance and oral glucose tolerance are alleviated by the regulation of blood glucose. Thus, the SH and UA extracts may be used in the development of therapeutic agents or supplements to improve insulin resistance.https://www.mdpi.com/1467-3045/45/9/473diabetes<i>Sargassum horneri</i><i>Ulva australis</i>extracts |
| spellingShingle | Young-Hyeon Lee Hye-Ran Kim Min-Ho Yeo Sung-Chun Kim Ho-Bong Hyun Young-Min Ham Yong-Hwan Jung Hye-Sook Kim Kyung-Soo Chang Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo Current Issues in Molecular Biology diabetes <i>Sargassum horneri</i> <i>Ulva australis</i> extracts |
| title | Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo |
| title_full | Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo |
| title_fullStr | Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo |
| title_full_unstemmed | Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo |
| title_short | Anti-Diabetic Potential of <i>Sargassum horneri</i> and <i>Ulva australis</i> Extracts In Vitro and In Vivo |
| title_sort | anti diabetic potential of i sargassum horneri i and i ulva australis i extracts in vitro and in vivo |
| topic | diabetes <i>Sargassum horneri</i> <i>Ulva australis</i> extracts |
| url | https://www.mdpi.com/1467-3045/45/9/473 |
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