Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast
Ethyl acetate is an important flavor element that is a vital component of <i>baijiu</i>. To date, the transcription factors that can help identify the molecular mechanisms involved in the synthesis of ethyl acetate have not been studied. In the present study, we sequenced and assembled t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/12/2341 |
| _version_ | 1797457858985459712 |
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| author | Bingqian Ni Weiwei Li Kiren Ifrah Binghao Du Youqiang Xu Chengnan Zhang Xiuting Li |
| author_facet | Bingqian Ni Weiwei Li Kiren Ifrah Binghao Du Youqiang Xu Chengnan Zhang Xiuting Li |
| author_sort | Bingqian Ni |
| collection | DOAJ |
| description | Ethyl acetate is an important flavor element that is a vital component of <i>baijiu</i>. To date, the transcription factors that can help identify the molecular mechanisms involved in the synthesis of ethyl acetate have not been studied. In the present study, we sequenced and assembled the <i>Wickerhamomyces anomalus</i> strain YF1503 transcriptomes to identify transcription factors. We identified 307 transcription factors in YF1503 using high-throughput RNA sequencing. Some transcription factors, such as C2H2, bHLH, MYB, and bZIP, were up-regulated, and these might play a role in ethyl acetate synthesis. According to the trend of ethyl acetate content, heat map results and STEM, twelve genes were selected for verification of expression levels using quantitative real-time PCR. This dynamic transcriptome analysis presents fundamental information on the transcription factors and pathways that are involved in the synthesis of ethyl acetate in aroma-producing yeast. Of significant interest is the discovery of the roles of various transcription factor genes in the synthesis of ethyl acetate. |
| first_indexed | 2024-03-09T16:28:49Z |
| format | Article |
| id | doaj.art-c7e131d6239745ed8836bf5748f8a6c2 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:28:49Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-c7e131d6239745ed8836bf5748f8a6c22023-11-24T15:05:18ZengMDPI AGGenes2073-44252022-12-011312234110.3390/genes13122341Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing YeastBingqian Ni0Weiwei Li1Kiren Ifrah2Binghao Du3Youqiang Xu4Chengnan Zhang5Xiuting Li6Key Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaKey Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce (BTBU), Beijing 100048, ChinaEthyl acetate is an important flavor element that is a vital component of <i>baijiu</i>. To date, the transcription factors that can help identify the molecular mechanisms involved in the synthesis of ethyl acetate have not been studied. In the present study, we sequenced and assembled the <i>Wickerhamomyces anomalus</i> strain YF1503 transcriptomes to identify transcription factors. We identified 307 transcription factors in YF1503 using high-throughput RNA sequencing. Some transcription factors, such as C2H2, bHLH, MYB, and bZIP, were up-regulated, and these might play a role in ethyl acetate synthesis. According to the trend of ethyl acetate content, heat map results and STEM, twelve genes were selected for verification of expression levels using quantitative real-time PCR. This dynamic transcriptome analysis presents fundamental information on the transcription factors and pathways that are involved in the synthesis of ethyl acetate in aroma-producing yeast. Of significant interest is the discovery of the roles of various transcription factor genes in the synthesis of ethyl acetate.https://www.mdpi.com/2073-4425/13/12/2341ethyl acetatetranscription factorRNA-Seqaroma-producing yeast |
| spellingShingle | Bingqian Ni Weiwei Li Kiren Ifrah Binghao Du Youqiang Xu Chengnan Zhang Xiuting Li Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast Genes ethyl acetate transcription factor RNA-Seq aroma-producing yeast |
| title | Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast |
| title_full | Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast |
| title_fullStr | Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast |
| title_full_unstemmed | Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast |
| title_short | Dynamic Transcriptome Analysis Reveals Transcription Factors Involved in the Synthesis of Ethyl Acetate in Aroma-Producing Yeast |
| title_sort | dynamic transcriptome analysis reveals transcription factors involved in the synthesis of ethyl acetate in aroma producing yeast |
| topic | ethyl acetate transcription factor RNA-Seq aroma-producing yeast |
| url | https://www.mdpi.com/2073-4425/13/12/2341 |
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