Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is t...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/7/1578 |
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| author | Agustina Mariana Portu María Sol Espain Silvia Inés Thorp Verónica Andrea Trivillin Paula Curotto Andrea Monti Hughes Emiliano César Cayetano Pozzi Marcela Alejandra Garabalino Mónica Alejandra Palmieri Pablo Nicolás Granell Federico Golmar Amanda Elena Schwint Gisela Saint Martin |
| author_facet | Agustina Mariana Portu María Sol Espain Silvia Inés Thorp Verónica Andrea Trivillin Paula Curotto Andrea Monti Hughes Emiliano César Cayetano Pozzi Marcela Alejandra Garabalino Mónica Alejandra Palmieri Pablo Nicolás Granell Federico Golmar Amanda Elena Schwint Gisela Saint Martin |
| author_sort | Agustina Mariana Portu |
| collection | DOAJ |
| description | The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT. |
| first_indexed | 2024-03-11T00:54:11Z |
| format | Article |
| id | doaj.art-c7e1afdfcff041f5855c70ab03b7e6eb |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T00:54:11Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-c7e1afdfcff041f5855c70ab03b7e6eb2023-11-18T20:10:30ZengMDPI AGLife2075-17292023-07-01137157810.3390/life13071578Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal ModelsAgustina Mariana Portu0María Sol Espain1Silvia Inés Thorp2Verónica Andrea Trivillin3Paula Curotto4Andrea Monti Hughes5Emiliano César Cayetano Pozzi6Marcela Alejandra Garabalino7Mónica Alejandra Palmieri8Pablo Nicolás Granell9Federico Golmar10Amanda Elena Schwint11Gisela Saint Martin12National Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaDepartment of Biodiversity and Experimental Biology, Faculty of Exact and Natural Sciences, University of Buenos Aires (UBA), Ciudad Autónoma de Buenos Aires C1428EGA, ArgentinaMicro and Nanotechnology Centre of the Bicentennial (CNMB), National Institute of Industrial Technology (INTI), San Martín B1650JKA, ArgentinaNational Scientific and Technological Research Council (CONICET), Ciudad Autónoma de Buenos Aires C1425FQB, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaNational Atomic Energy Commission (CNEA), San Martín C1429BNP, ArgentinaThe assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT.https://www.mdpi.com/2075-1729/13/7/1578neutron autoradiographynuclear track detectorsBNCTboron microdistributionanimal modelsUV-C |
| spellingShingle | Agustina Mariana Portu María Sol Espain Silvia Inés Thorp Verónica Andrea Trivillin Paula Curotto Andrea Monti Hughes Emiliano César Cayetano Pozzi Marcela Alejandra Garabalino Mónica Alejandra Palmieri Pablo Nicolás Granell Federico Golmar Amanda Elena Schwint Gisela Saint Martin Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models Life neutron autoradiography nuclear track detectors BNCT boron microdistribution animal models UV-C |
| title | Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models |
| title_full | Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models |
| title_fullStr | Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models |
| title_full_unstemmed | Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models |
| title_short | Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models |
| title_sort | enhanced resolution of neutron autoradiography with uv c sensitization to study boron microdistribution in animal models |
| topic | neutron autoradiography nuclear track detectors BNCT boron microdistribution animal models UV-C |
| url | https://www.mdpi.com/2075-1729/13/7/1578 |
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