Innate immune response to double-stranded RNA in American heritage chicken breeds
ABSTRACT: Backyard poultry flocks that employ heritage breeds of chicken play a crucial role in the maintenance of poultry pathogens of economic and zoonotic importance. This study examined innate immunity to viral pathogens in heritage chicken breeds using a model of viral double-stranded RNA (dsRN...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123008374 |
| _version_ | 1827354009318457344 |
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| author | Magda I. Abo-Samaha Mohammed M. Sharaf Abeer F. El Nahas Solomon O. Odemuyiwa |
| author_facet | Magda I. Abo-Samaha Mohammed M. Sharaf Abeer F. El Nahas Solomon O. Odemuyiwa |
| author_sort | Magda I. Abo-Samaha |
| collection | DOAJ |
| description | ABSTRACT: Backyard poultry flocks that employ heritage breeds of chicken play a crucial role in the maintenance of poultry pathogens of economic and zoonotic importance. This study examined innate immunity to viral pathogens in heritage chicken breeds using a model of viral double-stranded RNA (dsRNA). Following intraperitoneal injection of high molecular weight (HMW) -poly(I:C)/Lyovec into 4-wk-old chicks, we evaluated gene expression in peripheral blood mononuclear cells (PBMCs) and splenocytes. There was a significant difference across breeds in the expression of IL-4, IL-12p40, IFNγ, and B-cell activating factor (BAFF) in the spleen. In PBMCs, a significant difference in IFN-α expression was seen across breeds. Approximately 57% of IFN-α transcripts in PBMCs was explained by levels of expression of MDA5 transcripts. Using flow cytometry, we showed that only monocytes/macrophages (KUL01+ cells) expressed the scavenger receptor CD163. Regression analysis showed that 42% of fold change in CD163 expression on PBMCs was explained by breed (P < 0.0004). In general, breeds that responded to HMW-poly(I:C) by showing higher upregulation of IFNγ, IL-1β, and IL-12p40 transcripts in the spleen, and higher IFNα transcripts in peripheral blood, expressed less CD163 on blood monocytes. These findings suggest a genetic basis for the response of chickens to double-stranded RNA. Surface expression of the scavenger receptor CD163 in PBMCs following injection of high molecular weight poly(I:C) may be a rapid method to select chickens for breeding based on innate immune response to viral dsRNA. |
| first_indexed | 2024-03-08T03:36:33Z |
| format | Article |
| id | doaj.art-c7e1be8432f9472080a915ab5a91afa2 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T03:36:33Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c7e1be8432f9472080a915ab5a91afa22024-02-10T04:43:38ZengElsevierPoultry Science0032-57912024-02-011032103318Innate immune response to double-stranded RNA in American heritage chicken breedsMagda I. Abo-Samaha0Mohammed M. Sharaf1Abeer F. El Nahas2Solomon O. Odemuyiwa3Department of Pathobiology, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088; Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, EgyptDepartment of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, EgyptDepartment of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, EgyptDepartment of Pathobiology, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088; Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA; Corresponding author:ABSTRACT: Backyard poultry flocks that employ heritage breeds of chicken play a crucial role in the maintenance of poultry pathogens of economic and zoonotic importance. This study examined innate immunity to viral pathogens in heritage chicken breeds using a model of viral double-stranded RNA (dsRNA). Following intraperitoneal injection of high molecular weight (HMW) -poly(I:C)/Lyovec into 4-wk-old chicks, we evaluated gene expression in peripheral blood mononuclear cells (PBMCs) and splenocytes. There was a significant difference across breeds in the expression of IL-4, IL-12p40, IFNγ, and B-cell activating factor (BAFF) in the spleen. In PBMCs, a significant difference in IFN-α expression was seen across breeds. Approximately 57% of IFN-α transcripts in PBMCs was explained by levels of expression of MDA5 transcripts. Using flow cytometry, we showed that only monocytes/macrophages (KUL01+ cells) expressed the scavenger receptor CD163. Regression analysis showed that 42% of fold change in CD163 expression on PBMCs was explained by breed (P < 0.0004). In general, breeds that responded to HMW-poly(I:C) by showing higher upregulation of IFNγ, IL-1β, and IL-12p40 transcripts in the spleen, and higher IFNα transcripts in peripheral blood, expressed less CD163 on blood monocytes. These findings suggest a genetic basis for the response of chickens to double-stranded RNA. Surface expression of the scavenger receptor CD163 in PBMCs following injection of high molecular weight poly(I:C) may be a rapid method to select chickens for breeding based on innate immune response to viral dsRNA.http://www.sciencedirect.com/science/article/pii/S0032579123008374dsRNAcytokine transcriptCD163American heritage chicken |
| spellingShingle | Magda I. Abo-Samaha Mohammed M. Sharaf Abeer F. El Nahas Solomon O. Odemuyiwa Innate immune response to double-stranded RNA in American heritage chicken breeds Poultry Science dsRNA cytokine transcript CD163 American heritage chicken |
| title | Innate immune response to double-stranded RNA in American heritage chicken breeds |
| title_full | Innate immune response to double-stranded RNA in American heritage chicken breeds |
| title_fullStr | Innate immune response to double-stranded RNA in American heritage chicken breeds |
| title_full_unstemmed | Innate immune response to double-stranded RNA in American heritage chicken breeds |
| title_short | Innate immune response to double-stranded RNA in American heritage chicken breeds |
| title_sort | innate immune response to double stranded rna in american heritage chicken breeds |
| topic | dsRNA cytokine transcript CD163 American heritage chicken |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123008374 |
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