An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation
An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe<sub>3</sub>O<sub>4</sub> magnetic nanopartic...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/8/1155 |
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| author | Jie-Biao Guo Jin-Sheng Cheng Tai-Long Wei Fan-Min Wu Gui-Hong Tang Qing-Hua He |
| author_facet | Jie-Biao Guo Jin-Sheng Cheng Tai-Long Wei Fan-Min Wu Gui-Hong Tang Qing-Hua He |
| author_sort | Jie-Biao Guo |
| collection | DOAJ |
| description | An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL<sup>−1</sup> of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up. |
| first_indexed | 2024-03-09T10:36:39Z |
| format | Article |
| id | doaj.art-c7e3f01fbd934f759a7837de28709a74 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T10:36:39Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-c7e3f01fbd934f759a7837de28709a742023-12-01T20:55:06ZengMDPI AGFoods2304-81582022-04-01118115510.3390/foods11081155An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-ConfirmationJie-Biao Guo0Jin-Sheng Cheng1Tai-Long Wei2Fan-Min Wu3Gui-Hong Tang4Qing-Hua He5Provincial Key Laboratory for Utilization and Conservation of Food and Medicinal Research in Northern Guangdong, Shaoguan University, No. 288 Daxue Road, Shaoguan 512005, ChinaSchool of Innovation and Entrepreneurship, Shaoguan University, No. 288 Daxue Road, Shaoguan 512005, ChinaState Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, ChinaShaoguan Food and Drug Inspection Institute, No.13 Muxi Road, Shaoguan 512026, ChinaShaoguan Food and Drug Inspection Institute, No.13 Muxi Road, Shaoguan 512026, ChinaState Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, ChinaAn immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents. The immuno-separation of fluorescent reporters by magnetic adsorbents could be completed by ochratoxin A, so the fluorescent reporters released from the immune complex indicate a linear correlation with the concentration of ochratoxin A. Furthermore, the immuno-separated ochratoxin A can be eluted from magnetic adsorbent for LC-conformation. The optimized assay showed results as follows: the quantitative range of the immuno-separated assay was 0.03–100 ng mL<sup>−1</sup> of ochratoxin A. The recoveries for spiked samples ranged from 78.2% to 91.4%, with the relative standard deviation (RSD) being 11.9%~15.3%. Statistical analysis indicated no significant difference between the HPLC-FLD results based on commercial affinity column and by nano-affinity cleaning up.https://www.mdpi.com/2304-8158/11/8/1155immuno-separated assaynano-affinity cleaning upimmuno-magnetic adsorbentimmuno-fluorescent reportersLC-confirmation |
| spellingShingle | Jie-Biao Guo Jin-Sheng Cheng Tai-Long Wei Fan-Min Wu Gui-Hong Tang Qing-Hua He An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation Foods immuno-separated assay nano-affinity cleaning up immuno-magnetic adsorbent immuno-fluorescent reporters LC-confirmation |
| title | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
| title_full | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
| title_fullStr | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
| title_full_unstemmed | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
| title_short | An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation |
| title_sort | immuno separated assay for ochratoxin detection coupled with a nano affinity cleaning up for lc confirmation |
| topic | immuno-separated assay nano-affinity cleaning up immuno-magnetic adsorbent immuno-fluorescent reporters LC-confirmation |
| url | https://www.mdpi.com/2304-8158/11/8/1155 |
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