Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative
Cellular membranes play a key role in cell communication with the extracellular environment and neighboring cells. Any changes, including their composition, packing, physicochemical properties and formation of membrane protrusions may affect cells feature. Despite its great importance, tracking memb...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/11/9620 |
| _version_ | 1797597350599852032 |
|---|---|
| author | Aneta Rzewnicka Jerzy Krysiak Róża Pawłowska Remigiusz Żurawiński |
| author_facet | Aneta Rzewnicka Jerzy Krysiak Róża Pawłowska Remigiusz Żurawiński |
| author_sort | Aneta Rzewnicka |
| collection | DOAJ |
| description | Cellular membranes play a key role in cell communication with the extracellular environment and neighboring cells. Any changes, including their composition, packing, physicochemical properties and formation of membrane protrusions may affect cells feature. Despite its great importance, tracking membrane changes in living cells is still a challenge. For investigation of processes related to tissue regeneration and cancer metastasis, such as the induction of epithelial-mesenchymal transition, increased cell motility, and blebbing, the possibility to conduct prolonged observation of membrane changes is beneficial, albeit difficult. A particular challenge is conducting this type of research under detachment conditions. In the current manuscript, a new dithienothiophene <i>S</i>,<i>S</i>-dioxide (DTTDO) derivative is presented as an effective dye for staining the membranes of living cells. The synthetic procedures, physicochemical properties, and biological activity of the new compound are presented herein. In addition to the labeling of the membranes in a monolayer culture, its usefulness for visualization of membranes under detachment conditions is also demonstrated. Obtained data have proven that a new DTTDO derivative may be used to stain membranes in various types of experimental procedures, from traditional 2D cell cultures to unanchored conditions. Moreover, due to the specific optical properties, the background signal is reduced and, thus, observation may be performed without washing. |
| first_indexed | 2024-03-11T03:05:00Z |
| format | Article |
| id | doaj.art-c7eafc8280bc420b8984caccd027cd12 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:05:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c7eafc8280bc420b8984caccd027cd122023-11-18T08:01:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012411962010.3390/ijms24119620Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide DerivativeAneta Rzewnicka0Jerzy Krysiak1Róża Pawłowska2Remigiusz Żurawiński3Division of Organic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, PolandDivision of Organic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, PolandDivision of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, PolandDivision of Organic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, PolandCellular membranes play a key role in cell communication with the extracellular environment and neighboring cells. Any changes, including their composition, packing, physicochemical properties and formation of membrane protrusions may affect cells feature. Despite its great importance, tracking membrane changes in living cells is still a challenge. For investigation of processes related to tissue regeneration and cancer metastasis, such as the induction of epithelial-mesenchymal transition, increased cell motility, and blebbing, the possibility to conduct prolonged observation of membrane changes is beneficial, albeit difficult. A particular challenge is conducting this type of research under detachment conditions. In the current manuscript, a new dithienothiophene <i>S</i>,<i>S</i>-dioxide (DTTDO) derivative is presented as an effective dye for staining the membranes of living cells. The synthetic procedures, physicochemical properties, and biological activity of the new compound are presented herein. In addition to the labeling of the membranes in a monolayer culture, its usefulness for visualization of membranes under detachment conditions is also demonstrated. Obtained data have proven that a new DTTDO derivative may be used to stain membranes in various types of experimental procedures, from traditional 2D cell cultures to unanchored conditions. Moreover, due to the specific optical properties, the background signal is reduced and, thus, observation may be performed without washing.https://www.mdpi.com/1422-0067/24/11/9620bioimagingfluorescent dyemembrane stainingdithienothiophene |
| spellingShingle | Aneta Rzewnicka Jerzy Krysiak Róża Pawłowska Remigiusz Żurawiński Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative International Journal of Molecular Sciences bioimaging fluorescent dye membrane staining dithienothiophene |
| title | Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative |
| title_full | Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative |
| title_fullStr | Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative |
| title_full_unstemmed | Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative |
| title_short | Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene <i>S,S</i>-Dioxide Derivative |
| title_sort | visualization of cellular membranes in 2d and 3d conditions using a new fluorescent dithienothiophene i s s i dioxide derivative |
| topic | bioimaging fluorescent dye membrane staining dithienothiophene |
| url | https://www.mdpi.com/1422-0067/24/11/9620 |
| work_keys_str_mv | AT anetarzewnicka visualizationofcellularmembranesin2dand3dconditionsusinganewfluorescentdithienothiopheneissidioxidederivative AT jerzykrysiak visualizationofcellularmembranesin2dand3dconditionsusinganewfluorescentdithienothiopheneissidioxidederivative AT rozapawłowska visualizationofcellularmembranesin2dand3dconditionsusinganewfluorescentdithienothiopheneissidioxidederivative AT remigiuszzurawinski visualizationofcellularmembranesin2dand3dconditionsusinganewfluorescentdithienothiopheneissidioxidederivative |