Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis
Recent evidence suggests that the primary progressive form of multiple sclerosis (PP-MS) may present with specific immunological alterations. In this study we focused our attention on CD161, an NK and T cell marker upregulated in relapsing-remitting MS, and investigated its transcript and protein le...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-08-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01922/full |
| _version_ | 1831683841762263040 |
|---|---|
| author | Massimo Acquaviva Claudia Bassani Nicole Sarno Gloria Dalla Costa Marzia Romeo Francesca Sangalli Bruno Colombo Lucia Moiola Vittorio Martinelli Giancarlo Comi Cinthia Farina |
| author_facet | Massimo Acquaviva Claudia Bassani Nicole Sarno Gloria Dalla Costa Marzia Romeo Francesca Sangalli Bruno Colombo Lucia Moiola Vittorio Martinelli Giancarlo Comi Cinthia Farina |
| author_sort | Massimo Acquaviva |
| collection | DOAJ |
| description | Recent evidence suggests that the primary progressive form of multiple sclerosis (PP-MS) may present with specific immunological alterations. In this study we focused our attention on CD161, an NK and T cell marker upregulated in relapsing-remitting MS, and investigated its transcript and protein levels in blood cells from PP-MS and healthy individuals. We demonstrated transcriptional downregulation of CD161 in PP-MS and described concomitant mRNA reduction for RORgt, CCR6, CXCR6, KLRK1/NKG2D and many other markers typical of mucosa associated invariant T (MAIT) cells. Targeted multiparametric flow cytometry on fresh blood cells from an independent cohort of case-control subjects confirmed the selective loss of circulating CD8 CD161high T cells, which consist mainly of MAIT cells, and not of CD8 CD161int T cells in PP-MS. These data demonstrate alterations in a specific circulating immune cell subset in MS patients with progressive onset. |
| first_indexed | 2024-12-20T06:45:25Z |
| format | Article |
| id | doaj.art-c7ef3bad3bf346a4b87d2660178b8c49 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T06:45:25Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c7ef3bad3bf346a4b87d2660178b8c492022-12-21T19:49:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.01922451680Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple SclerosisMassimo AcquavivaClaudia BassaniNicole SarnoGloria Dalla CostaMarzia RomeoFrancesca SangalliBruno ColomboLucia MoiolaVittorio MartinelliGiancarlo ComiCinthia FarinaRecent evidence suggests that the primary progressive form of multiple sclerosis (PP-MS) may present with specific immunological alterations. In this study we focused our attention on CD161, an NK and T cell marker upregulated in relapsing-remitting MS, and investigated its transcript and protein levels in blood cells from PP-MS and healthy individuals. We demonstrated transcriptional downregulation of CD161 in PP-MS and described concomitant mRNA reduction for RORgt, CCR6, CXCR6, KLRK1/NKG2D and many other markers typical of mucosa associated invariant T (MAIT) cells. Targeted multiparametric flow cytometry on fresh blood cells from an independent cohort of case-control subjects confirmed the selective loss of circulating CD8 CD161high T cells, which consist mainly of MAIT cells, and not of CD8 CD161int T cells in PP-MS. These data demonstrate alterations in a specific circulating immune cell subset in MS patients with progressive onset.https://www.frontiersin.org/article/10.3389/fimmu.2019.01922/fullCD161CD8bloodprogressive multiple sclerosisMAIT cells |
| spellingShingle | Massimo Acquaviva Claudia Bassani Nicole Sarno Gloria Dalla Costa Marzia Romeo Francesca Sangalli Bruno Colombo Lucia Moiola Vittorio Martinelli Giancarlo Comi Cinthia Farina Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis Frontiers in Immunology CD161 CD8 blood progressive multiple sclerosis MAIT cells |
| title | Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis |
| title_full | Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis |
| title_fullStr | Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis |
| title_full_unstemmed | Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis |
| title_short | Loss of Circulating CD8+ CD161high T Cells in Primary Progressive Multiple Sclerosis |
| title_sort | loss of circulating cd8 cd161high t cells in primary progressive multiple sclerosis |
| topic | CD161 CD8 blood progressive multiple sclerosis MAIT cells |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01922/full |
| work_keys_str_mv | AT massimoacquaviva lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT claudiabassani lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT nicolesarno lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT gloriadallacosta lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT marziaromeo lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT francescasangalli lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT brunocolombo lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT luciamoiola lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT vittoriomartinelli lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT giancarlocomi lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis AT cinthiafarina lossofcirculatingcd8cd161hightcellsinprimaryprogressivemultiplesclerosis |