Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i>
Bioactivity with antifungal properties has already been reported for some species of the genus <i>Dipteryx</i>. However, little is known about <i>Dipteryx punctata</i>. We evaluated the antifungal activity, in vitro and in vivo, of extracts of leaves, branches and fruits of &...
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MDPI AG
2022-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/9/2215 |
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| author | Bruna Cristine Martins de Sousa Daniel do Amaral Gomes Thiago Almeida Vieira Denise Castro Lustosa |
| author_facet | Bruna Cristine Martins de Sousa Daniel do Amaral Gomes Thiago Almeida Vieira Denise Castro Lustosa |
| author_sort | Bruna Cristine Martins de Sousa |
| collection | DOAJ |
| description | Bioactivity with antifungal properties has already been reported for some species of the genus <i>Dipteryx</i>. However, little is known about <i>Dipteryx punctata</i>. We evaluated the antifungal activity, in vitro and in vivo, of extracts of leaves, branches and fruits of <i>D. punctata</i> on <i>Colletotrichum</i> <i>musae</i>, the causal agent of anthracnose in bananas. The extracts and the coumarin standard were tested in vitro, at concentrations of 10%, 20%, 30%, 40%, and 50% (<i>w</i>/<i>v</i>), added in potato-dextrose-agar (PDA) medium. The experimental design was completely randomized, factorial design, with four replications. The average diameter of the fungal colonies was evaluated daily for eight days, and at the end, the percentage of inhibition and growth rates were calculated. For in vivo tests, the products were tested at concentrations of 40% and 50%, in preventive and curative applications. In these tests, three medium disks (5 mm) containing phytopathogen structures were deposited at opposite points of the fruits, and remained in a humid chamber for 48 h. Anthracnose lesions were measured at an interval of two days, up to eight days after fungus inoculation. In the in vitro essay, the extracts of the branches and residues of the fruits of <i>D. punctata</i> and the coumarin pattern caused the biggest reductions in the average diameter of the colonies of the phytopathogen. <i>D. punctata</i> and coumarin extracts were promising in terms of in vivo antifungal activity, especially in preventive applications, being an important source of investigation for the formulation of natural products as sustainable phytosanitary measures. |
| first_indexed | 2024-03-10T00:59:01Z |
| format | Article |
| id | doaj.art-c7f28e138f6242618169a5a24ccda03f |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:59:01Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c7f28e138f6242618169a5a24ccda03f2023-11-23T14:39:16ZengMDPI AGAgronomy2073-43952022-09-01129221510.3390/agronomy12092215Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i>Bruna Cristine Martins de Sousa0Daniel do Amaral Gomes1Thiago Almeida Vieira2Denise Castro Lustosa3Instituto de Biodiversidade e Florestas (IBEF), Universidade Federal do Oeste do Pará (UFOPA), Santarém 68040-255, Pará, BrazilEscritorio Local de Mojuí dos Campos, Empresa de Assistência Técnica e Extensão Rural do Estado do Pará (EMATER), Mojuí dos Campos 68120-000, Pará, BrazilInstituto de Biodiversidade e Florestas (IBEF), Universidade Federal do Oeste do Pará (UFOPA), Santarém 68040-255, Pará, BrazilInstituto de Biodiversidade e Florestas (IBEF), Universidade Federal do Oeste do Pará (UFOPA), Santarém 68040-255, Pará, BrazilBioactivity with antifungal properties has already been reported for some species of the genus <i>Dipteryx</i>. However, little is known about <i>Dipteryx punctata</i>. We evaluated the antifungal activity, in vitro and in vivo, of extracts of leaves, branches and fruits of <i>D. punctata</i> on <i>Colletotrichum</i> <i>musae</i>, the causal agent of anthracnose in bananas. The extracts and the coumarin standard were tested in vitro, at concentrations of 10%, 20%, 30%, 40%, and 50% (<i>w</i>/<i>v</i>), added in potato-dextrose-agar (PDA) medium. The experimental design was completely randomized, factorial design, with four replications. The average diameter of the fungal colonies was evaluated daily for eight days, and at the end, the percentage of inhibition and growth rates were calculated. For in vivo tests, the products were tested at concentrations of 40% and 50%, in preventive and curative applications. In these tests, three medium disks (5 mm) containing phytopathogen structures were deposited at opposite points of the fruits, and remained in a humid chamber for 48 h. Anthracnose lesions were measured at an interval of two days, up to eight days after fungus inoculation. In the in vitro essay, the extracts of the branches and residues of the fruits of <i>D. punctata</i> and the coumarin pattern caused the biggest reductions in the average diameter of the colonies of the phytopathogen. <i>D. punctata</i> and coumarin extracts were promising in terms of in vivo antifungal activity, especially in preventive applications, being an important source of investigation for the formulation of natural products as sustainable phytosanitary measures.https://www.mdpi.com/2073-4395/12/9/2215anthracnosealternative controlbiological productsphytopathogenic fungi |
| spellingShingle | Bruna Cristine Martins de Sousa Daniel do Amaral Gomes Thiago Almeida Vieira Denise Castro Lustosa Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> Agronomy anthracnose alternative control biological products phytopathogenic fungi |
| title | Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> |
| title_full | Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> |
| title_fullStr | Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> |
| title_full_unstemmed | Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> |
| title_short | Bioactivity of Ethanolic Extracts of <i>Dipteryx punctata</i> on <i>Colletotrichum musae</i> |
| title_sort | bioactivity of ethanolic extracts of i dipteryx punctata i on i colletotrichum musae i |
| topic | anthracnose alternative control biological products phytopathogenic fungi |
| url | https://www.mdpi.com/2073-4395/12/9/2215 |
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