G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease
Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) f...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_7918_a417b9fe1c4b06c5d0648f44ab70dc93.pdf |
| _version_ | 1819038470933315584 |
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| author | Manouchehr Safari Behnaz Jafari Sam Zarbakhsh Hamidreza Sameni Abbas Ali Vafaei Nasrin Khan Mohammadi Laya Ghahari |
| author_facet | Manouchehr Safari Behnaz Jafari Sam Zarbakhsh Hamidreza Sameni Abbas Ali Vafaei Nasrin Khan Mohammadi Laya Ghahari |
| author_sort | Manouchehr Safari |
| collection | DOAJ |
| description | Objective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of BMSCs through the tail vein. Materials and Methods: We choose 25 male Wistar rats (200–250 g) were lesioned by 6-OHDA injected into the left substantia nigra, pars compacta (SNpc). G-CSF (70 µg/kg/day) was given from the 7th day after lesion for five days. The BMSCs (2×105) were injected through the dorsal tail vein on the 7th day after lesion. Results:The number of rotations was significantly lower in the stem cell therapy group than in the control group. In the third test in the received G-CSF and G-CSF+stem cells groups, animals displayed significant behavioral recovery compared with the control group (P |
| first_indexed | 2024-12-21T08:37:50Z |
| format | Article |
| id | doaj.art-c7f31d6ab476439ea6c60bbfbbfa5afc |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-21T08:37:50Z |
| publishDate | 2016-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c7f31d6ab476439ea6c60bbfbbfa5afc2022-12-21T19:10:02ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-12-0119121318132410.22038/ijbms.2016.79187918G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's diseaseManouchehr Safari0Behnaz Jafari1Sam Zarbakhsh2Hamidreza Sameni3Abbas Ali Vafaei4Nasrin Khan Mohammadi5Laya Ghahari6Research Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, IranDepartment of Physiology, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, Semnan University of Medical Sciences, Semnan, IranDepartment of Anatomy, AJA University of Medical Sciences, Tehran, IranObjective(s): Granulocyte-colony stimulating factor (G-CSF) is used in clinical practice for the treatment of neutropenia and to stimulate generation of hematopoietic stem cells in bone marrow donors. In the present study, the ability of G-CSF in mobilizing exogenous bone marrow stem cells (BMSCs) from peripheral blood into the brain was tested. We for the first time injected a small amount of BMSCs through the tail vein. Materials and Methods: We choose 25 male Wistar rats (200–250 g) were lesioned by 6-OHDA injected into the left substantia nigra, pars compacta (SNpc). G-CSF (70 µg/kg/day) was given from the 7th day after lesion for five days. The BMSCs (2×105) were injected through the dorsal tail vein on the 7th day after lesion. Results:The number of rotations was significantly lower in the stem cell therapy group than in the control group. In the third test in the received G-CSF and G-CSF+stem cells groups, animals displayed significant behavioral recovery compared with the control group (Phttp://ijbms.mums.ac.ir/article_7918_a417b9fe1c4b06c5d0648f44ab70dc93.pdfBone marrow stem cellsDopaminergic neuronsGranulocyte colony stimulating factorParkinson |
| spellingShingle | Manouchehr Safari Behnaz Jafari Sam Zarbakhsh Hamidreza Sameni Abbas Ali Vafaei Nasrin Khan Mohammadi Laya Ghahari G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease Iranian Journal of Basic Medical Sciences Bone marrow stem cells Dopaminergic neurons Granulocyte colony stimulating factor Parkinson |
| title | G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease |
| title_full | G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease |
| title_fullStr | G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease |
| title_full_unstemmed | G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease |
| title_short | G-CSF for mobilizing transplanted bone marrow stem cells in rat model of Parkinson's disease |
| title_sort | g csf for mobilizing transplanted bone marrow stem cells in rat model of parkinson s disease |
| topic | Bone marrow stem cells Dopaminergic neurons Granulocyte colony stimulating factor Parkinson |
| url | http://ijbms.mums.ac.ir/article_7918_a417b9fe1c4b06c5d0648f44ab70dc93.pdf |
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