Increased On-Target Rate and Risk of Concatemerization after CRISPR-Enhanced Targeting in ES Cells

Increased On-Target Rate and Risk of Concatemerization after CRISPR-Enhanced Targeting in ES Cells

The French mouse clinic (Institut Clinique de la Souris; ICS) has produced more than 2000 targeting vectors for ‘à la carte’ mutagenesis in C57BL/6N mice. Although most of the vectors were used successfully for homologous recombination in murine embryonic stem cells (ESCs), a few have failed to targ...

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Bibliographic Details
Main Authors: Valérie Erbs, Romain Lorentz, Benjamin Eisenman, Laurence Schaeffer, Laurence Luppi, Loic Lindner, Yann Hérault, Guillaume Pavlovic, Marie Wattenhofer-Donzé, Marie-Christine Birling
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Genes
Subjects:
gene targeting
double-strand break
embryonic stem cells
genome editing
CRISPR/Cas9
homologous recombination
Online Access:https://www.mdpi.com/2073-4425/14/2/401

Internet

https://www.mdpi.com/2073-4425/14/2/401

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