Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression
Siderophores are iron-chelating agents produced by almost all microorganisms in response to iron deficiency. Due to the requirement of iron for cell growth and metabolism, siderophore-mediated acquisition of iron plays a central role in determining the ability of different microorganisms to coloniz...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2024-03-01
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| Series: | Notulae Scientia Biologicae |
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| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11555 |
| _version_ | 1797231362870083584 |
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| author | Redouan QESSAOUI Mohamed ELAAlAOUI Salahddine CHAFIKI Mohamed ALOUANI Naima CHABBI Naima AIT ABD Abdelghani TAHIRI Rachid BOUHARROUD |
| author_facet | Redouan QESSAOUI Mohamed ELAAlAOUI Salahddine CHAFIKI Mohamed ALOUANI Naima CHABBI Naima AIT ABD Abdelghani TAHIRI Rachid BOUHARROUD |
| author_sort | Redouan QESSAOUI |
| collection | DOAJ |
| description |
Siderophores are iron-chelating agents produced by almost all microorganisms in response to iron deficiency. Due to the requirement of iron for cell growth and metabolism, siderophore-mediated acquisition of iron plays a central role in determining the ability of different microorganisms to colonize plant roots and contributes to microbial interactions in the plant rhizosphere. In this study, five new Pseudomonas (Q14B, Q13B, Q7B, Q6B, Q1B), isolated from the rhizosphere of tomato in Morocco, were examined for siderophores production capacity. The results show that all five isolates produced siderophores on both solid and liquid mediums. In liquid medium, the highest level of production is obtained by Q13B (53.8%). Concerning siderophores’ chemical types, the five strains of Pseudomonas produce two types of siderophores hydroxamate and catecholate. It was shown by the peaks of absorbance in the wavelength 495 and between 420-450 nm for catecholate and hydroxamate-type siderophores respectively. The results showed that the production of siderophores is progressively inhibited with increasing concentrations of iron in the medium. The maximum production was obtained with a concentration of 0.5 μM, while the lowest was recorded at 10.0 μM of iron. The results of this study showed that the five Pseudomonas isolates producing siderophores could be potential biological control agents.
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| first_indexed | 2024-04-24T15:43:11Z |
| format | Article |
| id | doaj.art-c7f5289a21c94791978eca33be52978c |
| institution | Directory Open Access Journal |
| issn | 2067-3264 |
| language | English |
| last_indexed | 2024-04-24T15:43:11Z |
| publishDate | 2024-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-c7f5289a21c94791978eca33be52978c2024-04-01T18:32:40ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642024-03-0116110.55779/nsb16111555Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppressionRedouan QESSAOUI0Mohamed ELAAlAOUI1Salahddine CHAFIKI2Mohamed ALOUANI3Naima CHABBI4Naima AIT ABD5Abdelghani TAHIRI6Rachid BOUHARROUD7Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Settat, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Ibn Zohr University, Faculty of Sciences, Laboratory of Biotechnologies and Valorization of Natural Resources, AgadirRegional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090 Siderophores are iron-chelating agents produced by almost all microorganisms in response to iron deficiency. Due to the requirement of iron for cell growth and metabolism, siderophore-mediated acquisition of iron plays a central role in determining the ability of different microorganisms to colonize plant roots and contributes to microbial interactions in the plant rhizosphere. In this study, five new Pseudomonas (Q14B, Q13B, Q7B, Q6B, Q1B), isolated from the rhizosphere of tomato in Morocco, were examined for siderophores production capacity. The results show that all five isolates produced siderophores on both solid and liquid mediums. In liquid medium, the highest level of production is obtained by Q13B (53.8%). Concerning siderophores’ chemical types, the five strains of Pseudomonas produce two types of siderophores hydroxamate and catecholate. It was shown by the peaks of absorbance in the wavelength 495 and between 420-450 nm for catecholate and hydroxamate-type siderophores respectively. The results showed that the production of siderophores is progressively inhibited with increasing concentrations of iron in the medium. The maximum production was obtained with a concentration of 0.5 μM, while the lowest was recorded at 10.0 μM of iron. The results of this study showed that the five Pseudomonas isolates producing siderophores could be potential biological control agents. https://www.notulaebiologicae.ro/index.php/nsb/article/view/11555catecholatehydroxamatePseudomonassiderophores |
| spellingShingle | Redouan QESSAOUI Mohamed ELAAlAOUI Salahddine CHAFIKI Mohamed ALOUANI Naima CHABBI Naima AIT ABD Abdelghani TAHIRI Rachid BOUHARROUD Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression Notulae Scientia Biologicae catecholate hydroxamate Pseudomonas siderophores |
| title | Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression |
| title_full | Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression |
| title_fullStr | Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression |
| title_full_unstemmed | Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression |
| title_short | Pseudomonas siderophores: production, spectrophotometry detection and Botrytis suppression |
| title_sort | pseudomonas siderophores production spectrophotometry detection and botrytis suppression |
| topic | catecholate hydroxamate Pseudomonas siderophores |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11555 |
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