First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon
Piroplasms infections are tick-borne diseases caused by haemoparasite of the genus Theileria or Babesia. They have a great impact on livestock production, especially cattle in sub-Saharan countries. However, data on the prevalence of bovine piroplasms and their genetic diversity are scanty in Came...
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University of Tabriz
2021-09-01
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| Series: | Journal of Zoonotic Diseases |
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| Online Access: | https://jzd.tabrizu.ac.ir/article_13471_aa690d83c731912548ea3486bb15a3a9.pdf |
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| author | Ghislain Roméo Ngangnang Münir Aktas Mehmet Can Ulucesme Keptcheu Tchankwe Désiré Florence Fonteh Anyangwe Vincent Khan Payne |
| author_facet | Ghislain Roméo Ngangnang Münir Aktas Mehmet Can Ulucesme Keptcheu Tchankwe Désiré Florence Fonteh Anyangwe Vincent Khan Payne |
| author_sort | Ghislain Roméo Ngangnang |
| collection | DOAJ |
| description | Piroplasms infections are tick-borne diseases caused by haemoparasite of the genus Theileria or Babesia. They
have a great impact on livestock production, especially cattle in sub-Saharan countries. However, data on the
prevalence of bovine piroplasms and their genetic diversity are scanty in Cameroon. This study was aimed at
highlight the species composition and determine the prevalence of piroplasms infecting cattle in the Western
Highlands of Cameroon. To achieve this aim, blood samples from a total of 162 cattle were collected and
examined using Reverse Line Blot hybridization (RLB) assay. The amplified hypervariable V4 region of the
18S rRNA gene of bovine piroplasms species, including Theileria parva, T. annulata, T mutans, T. velifera,
T. buffeli/T. orientalis, T. taurotragi, Theileria sp (buffalo), Babesia bovis, B. bigemina, B. divergens, B. major
and B. occultans was hybridized against species-specific probes. RLB hybridization assay revealed the
presence of four piroplasms species with the overall prevalence of infection of 82.1%. Theileria velifera
(71.6%) was the most prevalent species followed by Theileria mutans (43.21%), Theileria buffeli/T. orientalis
(5.55%) and Babesia bigemina (3.7%). However, the study provided the first molecular evidence for the
presence of T. buffeli/T. orientalis group species in cattle in Cameroon. Higher overall prevalence of infection
of tick-borne pathogens was observed in this study area as well as the increase in prevalence and widespread
of T. velifera and the observance of a new species of piroplasms. These results are an indication that special
attention should be given to epizootiological investigations alongside well-adopted control programs. |
| first_indexed | 2024-12-18T10:54:56Z |
| format | Article |
| id | doaj.art-c7f5e1b0c0b3475092d29f6cf866d7ec |
| institution | Directory Open Access Journal |
| issn | 2717-2910 |
| language | English |
| last_indexed | 2024-12-18T10:54:56Z |
| publishDate | 2021-09-01 |
| publisher | University of Tabriz |
| record_format | Article |
| series | Journal of Zoonotic Diseases |
| spelling | doaj.art-c7f5e1b0c0b3475092d29f6cf866d7ec2022-12-21T21:10:21ZengUniversity of TabrizJournal of Zoonotic Diseases2717-29102021-09-0153435310.22034/JZD.2021.13471First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of CameroonGhislain Roméo Ngangnang0Münir Aktas1Mehmet Can Ulucesme2Keptcheu Tchankwe Désiré3Florence Fonteh Anyangwe4Vincent Khan Payne5Department of Animal Biology, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Parasitology, Faculty of Veterinary Medicine, University of Firät, Elazig, TurkeyDepartment of Parasitology, Faculty of Veterinary Medicine, University of Firät, Elazig, TurkeyDepartment of Animal Biology, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Department of Animal Biology, Faculty of Science, University of Dschang, Dschang, CameroonPiroplasms infections are tick-borne diseases caused by haemoparasite of the genus Theileria or Babesia. They have a great impact on livestock production, especially cattle in sub-Saharan countries. However, data on the prevalence of bovine piroplasms and their genetic diversity are scanty in Cameroon. This study was aimed at highlight the species composition and determine the prevalence of piroplasms infecting cattle in the Western Highlands of Cameroon. To achieve this aim, blood samples from a total of 162 cattle were collected and examined using Reverse Line Blot hybridization (RLB) assay. The amplified hypervariable V4 region of the 18S rRNA gene of bovine piroplasms species, including Theileria parva, T. annulata, T mutans, T. velifera, T. buffeli/T. orientalis, T. taurotragi, Theileria sp (buffalo), Babesia bovis, B. bigemina, B. divergens, B. major and B. occultans was hybridized against species-specific probes. RLB hybridization assay revealed the presence of four piroplasms species with the overall prevalence of infection of 82.1%. Theileria velifera (71.6%) was the most prevalent species followed by Theileria mutans (43.21%), Theileria buffeli/T. orientalis (5.55%) and Babesia bigemina (3.7%). However, the study provided the first molecular evidence for the presence of T. buffeli/T. orientalis group species in cattle in Cameroon. Higher overall prevalence of infection of tick-borne pathogens was observed in this study area as well as the increase in prevalence and widespread of T. velifera and the observance of a new species of piroplasms. These results are an indication that special attention should be given to epizootiological investigations alongside well-adopted control programs.https://jzd.tabrizu.ac.ir/article_13471_aa690d83c731912548ea3486bb15a3a9.pdfpiroplasmstheileria buffelibabesiarlbprevalencecameroon |
| spellingShingle | Ghislain Roméo Ngangnang Münir Aktas Mehmet Can Ulucesme Keptcheu Tchankwe Désiré Florence Fonteh Anyangwe Vincent Khan Payne First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon Journal of Zoonotic Diseases piroplasms theileria buffeli babesia rlb prevalence cameroon |
| title | First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon |
| title_full | First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon |
| title_fullStr | First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon |
| title_full_unstemmed | First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon |
| title_short | First report of Theileria buffeli/Theileria orientalis group and identification of piroplasms via Nested PCR-based RLB Hybridization assay in zebu cattle in the Western Highlands of Cameroon |
| title_sort | first report of theileria buffeli theileria orientalis group and identification of piroplasms via nested pcr based rlb hybridization assay in zebu cattle in the western highlands of cameroon |
| topic | piroplasms theileria buffeli babesia rlb prevalence cameroon |
| url | https://jzd.tabrizu.ac.ir/article_13471_aa690d83c731912548ea3486bb15a3a9.pdf |
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