First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda
Hemoplasmas (hemotropic mycoplasmas) are small pleomorphic bacteria that parasitize the surface of red blood cells of several mammalian species including cattle, goats, and humans, causing infectious anemia. However, studies on hemoplasmas have been neglected and to date, there are no studies on bov...
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MDPI AG
2020-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1624 |
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| author | Benedicto Byamukama Maria Agnes Tumwebaze Dickson Stuart Tayebwa Joseph Byaruhanga Martin Kamilo Angwe Jixu Li Eloiza May Galon Mingming Liu Yongchang Li Shengwei Ji Paul Frank Adjou Moumouni Aaron Ringo Seung-Hun Lee Patrick Vudriko Xuenan Xuan |
| author_facet | Benedicto Byamukama Maria Agnes Tumwebaze Dickson Stuart Tayebwa Joseph Byaruhanga Martin Kamilo Angwe Jixu Li Eloiza May Galon Mingming Liu Yongchang Li Shengwei Ji Paul Frank Adjou Moumouni Aaron Ringo Seung-Hun Lee Patrick Vudriko Xuenan Xuan |
| author_sort | Benedicto Byamukama |
| collection | DOAJ |
| description | Hemoplasmas (hemotropic mycoplasmas) are small pleomorphic bacteria that parasitize the surface of red blood cells of several mammalian species including cattle, goats, and humans, causing infectious anemia. However, studies on hemoplasmas have been neglected and to date, there are no studies on bovine and caprine hemoplasmas in Uganda or the entire East African region. In this study, a polymerase chain reaction (PCR) assay targeting the 16S rRNA gene was used to investigate the presence of hemoplasma in 409 samples (cattle = 208; goats = 201) collected from Kasese district, western Uganda. Results showed that 32.2% (67/208) of cattle samples and 43.8% (88/201) of goat samples were positive for hemoplasmas. Sequencing analysis identified <i>Candidatus</i> Mycoplasma haemobos and <i>Mycoplasma wenyonii</i> in cattle, while <i>Candidatus</i> Mycoplasma erythrocervae and <i>Mycoplasma ovis</i> were identified in goats. Statistical analysis showed that goats were at a higher risk of infection with hemoplasmas compared with cattle. To the best of our knowledge, this is the first molecular evidence of hemoplasmas in bovine and caprine animals in Uganda and the entire east African region. |
| first_indexed | 2024-03-10T16:25:49Z |
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| id | doaj.art-c7f607b373014215a2a1d0538a92a848 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T16:25:49Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c7f607b373014215a2a1d0538a92a8482023-11-20T13:18:31ZengMDPI AGAnimals2076-26152020-09-01109162410.3390/ani10091624First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from UgandaBenedicto Byamukama0Maria Agnes Tumwebaze1Dickson Stuart Tayebwa2Joseph Byaruhanga3Martin Kamilo Angwe4Jixu Li5Eloiza May Galon6Mingming Liu7Yongchang Li8Shengwei Ji9Paul Frank Adjou Moumouni10Aaron Ringo11Seung-Hun Lee12Patrick Vudriko13Xuenan Xuan14National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanResearch Center for Tropical Diseases and Vector Control, School of Veterinary Medicine and Animal Resources, Makerere University, Kampala 7062, UgandaResearch Center for Tropical Diseases and Vector Control, School of Veterinary Medicine and Animal Resources, Makerere University, Kampala 7062, UgandaResearch Center for Tropical Diseases and Vector Control, School of Veterinary Medicine and Animal Resources, Makerere University, Kampala 7062, UgandaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, KoreaResearch Center for Tropical Diseases and Vector Control, School of Veterinary Medicine and Animal Resources, Makerere University, Kampala 7062, UgandaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanHemoplasmas (hemotropic mycoplasmas) are small pleomorphic bacteria that parasitize the surface of red blood cells of several mammalian species including cattle, goats, and humans, causing infectious anemia. However, studies on hemoplasmas have been neglected and to date, there are no studies on bovine and caprine hemoplasmas in Uganda or the entire East African region. In this study, a polymerase chain reaction (PCR) assay targeting the 16S rRNA gene was used to investigate the presence of hemoplasma in 409 samples (cattle = 208; goats = 201) collected from Kasese district, western Uganda. Results showed that 32.2% (67/208) of cattle samples and 43.8% (88/201) of goat samples were positive for hemoplasmas. Sequencing analysis identified <i>Candidatus</i> Mycoplasma haemobos and <i>Mycoplasma wenyonii</i> in cattle, while <i>Candidatus</i> Mycoplasma erythrocervae and <i>Mycoplasma ovis</i> were identified in goats. Statistical analysis showed that goats were at a higher risk of infection with hemoplasmas compared with cattle. To the best of our knowledge, this is the first molecular evidence of hemoplasmas in bovine and caprine animals in Uganda and the entire east African region.https://www.mdpi.com/2076-2615/10/9/1624hemoplasmacattlegoatsPCRUganda |
| spellingShingle | Benedicto Byamukama Maria Agnes Tumwebaze Dickson Stuart Tayebwa Joseph Byaruhanga Martin Kamilo Angwe Jixu Li Eloiza May Galon Mingming Liu Yongchang Li Shengwei Ji Paul Frank Adjou Moumouni Aaron Ringo Seung-Hun Lee Patrick Vudriko Xuenan Xuan First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda Animals hemoplasma cattle goats PCR Uganda |
| title | First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda |
| title_full | First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda |
| title_fullStr | First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda |
| title_full_unstemmed | First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda |
| title_short | First Molecular Detection and Characterization of Hemotropic <i>Mycoplasma</i> Species in Cattle and Goats from Uganda |
| title_sort | first molecular detection and characterization of hemotropic i mycoplasma i species in cattle and goats from uganda |
| topic | hemoplasma cattle goats PCR Uganda |
| url | https://www.mdpi.com/2076-2615/10/9/1624 |
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