Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin
Purpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ). Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200±25 g) were divided...
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| Format: | Article |
| Language: | English |
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Cukurova University
2020-12-01
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| Series: | Cukurova Medical Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/1277266 |
| _version_ | 1797911339527569408 |
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| author | Şamil Öztürk Suat Çakına |
| author_facet | Şamil Öztürk Suat Çakına |
| author_sort | Şamil Öztürk |
| collection | DOAJ |
| description | Purpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ).
Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200±25 g) were divided into five groups (with seven rats each) and treated as follows: control (Cont), streptozotocin alone (STZ), streptozotocin + metformin (STZ+MET), streptozotocin + sitagliptin (STZ+STG), streptozotocin + metformin + sitagliptin (STZ+MET+STG). Sitagliptin, Metformin, and combined metformin sitagliptin treated subgroups for four weeks. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels, and oxidative stress index (OSI) ratio were measured in rat liver tissue. Besides, cells undergoing apoptotic cell death were determined using the TUNEL technique through histopathological evaluation.
Results: MDA, TAS, and OSI of STZ+MET+STG administered group decreased compared to STZ. TOS of STZ+MET+STG administered group decreased compared to STZ.
Conclusion: In the experimental T2D model in rats, it shows protective effect when sitagliptin is used with metformin against oxidative damage in liver tissue caused by STZ. |
| first_indexed | 2024-04-10T11:39:02Z |
| format | Article |
| id | doaj.art-c7f73452e50646909a58e982d0bb6a4d |
| institution | Directory Open Access Journal |
| issn | 2602-3040 |
| language | English |
| last_indexed | 2024-04-10T11:39:02Z |
| publishDate | 2020-12-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Cukurova Medical Journal |
| spelling | doaj.art-c7f73452e50646909a58e982d0bb6a4d2023-02-15T16:17:37ZengCukurova UniversityCukurova Medical Journal2602-30402020-12-014541733174110.17826/cumj.79136948Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptinŞamil Öztürk0Suat Çakına1CANAKKALE ONSEKIZ MART UNIVERSITY, ÇANAKKALE HEALTH SERVICES VOCATIONAL SCHOOLCANAKKALE ONSEKIZ MART UNIVERSITY, ÇANAKKALE HEALTH SERVICES VOCATIONAL SCHOOLPurpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ). Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200±25 g) were divided into five groups (with seven rats each) and treated as follows: control (Cont), streptozotocin alone (STZ), streptozotocin + metformin (STZ+MET), streptozotocin + sitagliptin (STZ+STG), streptozotocin + metformin + sitagliptin (STZ+MET+STG). Sitagliptin, Metformin, and combined metformin sitagliptin treated subgroups for four weeks. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels, and oxidative stress index (OSI) ratio were measured in rat liver tissue. Besides, cells undergoing apoptotic cell death were determined using the TUNEL technique through histopathological evaluation. Results: MDA, TAS, and OSI of STZ+MET+STG administered group decreased compared to STZ. TOS of STZ+MET+STG administered group decreased compared to STZ. Conclusion: In the experimental T2D model in rats, it shows protective effect when sitagliptin is used with metformin against oxidative damage in liver tissue caused by STZ.https://dergipark.org.tr/tr/download/article-file/1277266metforminsitagliptinoxidative stressdiabetmetforminsitagliptinoksidatif stresdiyabet |
| spellingShingle | Şamil Öztürk Suat Çakına Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin Cukurova Medical Journal metformin sitagliptin oxidative stress diabet metformin sitagliptin oksidatif stres diyabet |
| title | Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin |
| title_full | Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin |
| title_fullStr | Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin |
| title_full_unstemmed | Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin |
| title_short | Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin |
| title_sort | oxidative stress markers in liver in streptozocin induced diabetic rats effects of metformin and sitagliptin |
| topic | metformin sitagliptin oxidative stress diabet metformin sitagliptin oksidatif stres diyabet |
| url | https://dergipark.org.tr/tr/download/article-file/1277266 |
| work_keys_str_mv | AT samilozturk oxidativestressmarkersinliverinstreptozocininduceddiabeticratseffectsofmetforminandsitagliptin AT suatcakına oxidativestressmarkersinliverinstreptozocininduceddiabeticratseffectsofmetforminandsitagliptin |