Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles
Various phthalic acid esters (PAEs) such as dibutyl phthalate (DBP) and butyl benzyl phthalate (BBP) co-exist with nanopollutants in aquatic environment. In this study, Daphnia magna was exposed to nano-CuO and DBP or BBP at environmental relevant concentrations for 21-days to investigate these comb...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324004147 |
| _version_ | 1827257209967345664 |
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| author | Xu Jin Junlan Pan Cheng Zhang Xuesong Cao Chuanxi Wang Le Yue Xiaona Li Yinglin Liu Zhenyu Wang |
| author_facet | Xu Jin Junlan Pan Cheng Zhang Xuesong Cao Chuanxi Wang Le Yue Xiaona Li Yinglin Liu Zhenyu Wang |
| author_sort | Xu Jin |
| collection | DOAJ |
| description | Various phthalic acid esters (PAEs) such as dibutyl phthalate (DBP) and butyl benzyl phthalate (BBP) co-exist with nanopollutants in aquatic environment. In this study, Daphnia magna was exposed to nano-CuO and DBP or BBP at environmental relevant concentrations for 21-days to investigate these combined toxic effects. Acute EC50 values (48 h) of nano-CuO, DBP, and BBP were 12.572 mg/L, 8.978 mg/L, and 4.785 mg/L, respectively. Results showed that co-exposure with nano-CuO (500 μg/L) for 21 days significantly enhanced the toxicity of DBP (100 μg/L) and BBP (100 μg/L) to Daphnia magna by 18.37% and 18.11%, respectively. The activities of superoxide dismutase, catalase, and glutathione S-transferase were enhanced by 10.95% and 14.07%, 25.63% and 25.91%, and 39.93% and 35.01% in nano-CuO+DBP and nano-CuO+BBP treatments as compared to the individual exposure groups, verifying that antioxidative defense responses were activated. Furthermore, the co-exposure of nano-CuO and PAEs decreased the population richness and diversity microbiota, and changed the microbial community composition in Daphnia magna. Metabolomic analysis elucidated that nano-CuO + PAEs exposure induced stronger disturbance on metabolic network and molecular function, including amino acid, nucleotides, and lipid metabolism-related metabolic pathways, as comparison to PAEs single exposure treatments. In summary, the integration of physiological, microflora, and untargeted metabolomics analysis offers a fresh perspective into the potential ecological risk associated with nanopollutants and phthalate pollution in aquatic ecosystems. |
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| id | doaj.art-c7fa4c567cbb4870a6de18728021270c |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2025-03-22T01:51:10Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-c7fa4c567cbb4870a6de18728021270c2024-05-08T04:07:12ZengElsevierEcotoxicology and Environmental Safety0147-65132024-06-01277116338Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profilesXu Jin0Junlan Pan1Cheng Zhang2Xuesong Cao3Chuanxi Wang4Le Yue5Xiaona Li6Yinglin Liu7Zhenyu Wang8College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, ChinaInstitute of Environmental Processes and Pollution Control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, and Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China; Corresponding author at: Institute of Environmental Processes and Pollution control, and School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.Various phthalic acid esters (PAEs) such as dibutyl phthalate (DBP) and butyl benzyl phthalate (BBP) co-exist with nanopollutants in aquatic environment. In this study, Daphnia magna was exposed to nano-CuO and DBP or BBP at environmental relevant concentrations for 21-days to investigate these combined toxic effects. Acute EC50 values (48 h) of nano-CuO, DBP, and BBP were 12.572 mg/L, 8.978 mg/L, and 4.785 mg/L, respectively. Results showed that co-exposure with nano-CuO (500 μg/L) for 21 days significantly enhanced the toxicity of DBP (100 μg/L) and BBP (100 μg/L) to Daphnia magna by 18.37% and 18.11%, respectively. The activities of superoxide dismutase, catalase, and glutathione S-transferase were enhanced by 10.95% and 14.07%, 25.63% and 25.91%, and 39.93% and 35.01% in nano-CuO+DBP and nano-CuO+BBP treatments as compared to the individual exposure groups, verifying that antioxidative defense responses were activated. Furthermore, the co-exposure of nano-CuO and PAEs decreased the population richness and diversity microbiota, and changed the microbial community composition in Daphnia magna. Metabolomic analysis elucidated that nano-CuO + PAEs exposure induced stronger disturbance on metabolic network and molecular function, including amino acid, nucleotides, and lipid metabolism-related metabolic pathways, as comparison to PAEs single exposure treatments. In summary, the integration of physiological, microflora, and untargeted metabolomics analysis offers a fresh perspective into the potential ecological risk associated with nanopollutants and phthalate pollution in aquatic ecosystems.http://www.sciencedirect.com/science/article/pii/S0147651324004147CuO nanoparticlesPhthalic acid estersCo-exposureMicrobiomeMetabolic network |
| spellingShingle | Xu Jin Junlan Pan Cheng Zhang Xuesong Cao Chuanxi Wang Le Yue Xiaona Li Yinglin Liu Zhenyu Wang Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles Ecotoxicology and Environmental Safety CuO nanoparticles Phthalic acid esters Co-exposure Microbiome Metabolic network |
| title | Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles |
| title_full | Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles |
| title_fullStr | Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles |
| title_full_unstemmed | Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles |
| title_short | Toxic mechanism in Daphnia magna due to phthalic acid esters and CuO nanoparticles co-exposure: The insight of physiological, microbiomic and metabolomic profiles |
| title_sort | toxic mechanism in daphnia magna due to phthalic acid esters and cuo nanoparticles co exposure the insight of physiological microbiomic and metabolomic profiles |
| topic | CuO nanoparticles Phthalic acid esters Co-exposure Microbiome Metabolic network |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324004147 |
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