Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise m...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2014-02-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200101&lng=en&tlng=en |
| _version_ | 1811201088890601472 |
|---|---|
| author | R. Yang Q. Lin H.B. Gao P. Zhang |
| author_facet | R. Yang Q. Lin H.B. Gao P. Zhang |
| author_sort | R. Yang |
| collection | DOAJ |
| description | In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone norepinephrine (NE) is not clear, and, furthermore, there are no reports about the effect of NE on IL-6 expression in gastric epithelial cells. In this study, we examined the effect of NE on IL-6 expression in immortalized human gastric epithelial cells (GES-1 cells). Using real-time PCR and enzyme-linked immunoassay, we demonstrated that NE can induce IL-6 mRNA and protein expression in GES-1 cells. The induction is through the β-adrenergic receptor-cAMP-protein kinase A pathway and mainly at the transcriptional level. Progressive 5′-deletions and site-directed mutagenesis of the parental construct show that, although activating-protein-1 (AP-1), cAMP-responsive element binding protein (CREB), CCAAT-enhancer binding protein-β (C/EBP-β), and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) binding sites are all required in the basal transcription of IL-6, only AP-1 and CREB binding sites in the IL-6 promoter are required in NE-induced IL-6 expression. The results suggest that chronic stress may increase IL-6 secretion of human gastric epithelial cells, at least in part, by the stress-associated hormone norepinephrine, and provides basic data on stress and gastric cancer progression. |
| first_indexed | 2024-04-12T02:15:00Z |
| format | Article |
| id | doaj.art-c7fbfa9d831f409a842f3378938cbe9b |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-12T02:15:00Z |
| publishDate | 2014-02-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-c7fbfa9d831f409a842f3378938cbe9b2022-12-22T03:52:17ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2014-02-0147210110910.1590/1414-431X20133346S0100-879X2014000200101Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cellsR. YangQ. LinH.B. GaoP. ZhangIn the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone norepinephrine (NE) is not clear, and, furthermore, there are no reports about the effect of NE on IL-6 expression in gastric epithelial cells. In this study, we examined the effect of NE on IL-6 expression in immortalized human gastric epithelial cells (GES-1 cells). Using real-time PCR and enzyme-linked immunoassay, we demonstrated that NE can induce IL-6 mRNA and protein expression in GES-1 cells. The induction is through the β-adrenergic receptor-cAMP-protein kinase A pathway and mainly at the transcriptional level. Progressive 5′-deletions and site-directed mutagenesis of the parental construct show that, although activating-protein-1 (AP-1), cAMP-responsive element binding protein (CREB), CCAAT-enhancer binding protein-β (C/EBP-β), and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) binding sites are all required in the basal transcription of IL-6, only AP-1 and CREB binding sites in the IL-6 promoter are required in NE-induced IL-6 expression. The results suggest that chronic stress may increase IL-6 secretion of human gastric epithelial cells, at least in part, by the stress-associated hormone norepinephrine, and provides basic data on stress and gastric cancer progression.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200101&lng=en&tlng=enNorepinephrineGES-1 cellsInterleukin-6 |
| spellingShingle | R. Yang Q. Lin H.B. Gao P. Zhang Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells Brazilian Journal of Medical and Biological Research Norepinephrine GES-1 cells Interleukin-6 |
| title | Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells |
| title_full | Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells |
| title_fullStr | Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells |
| title_full_unstemmed | Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells |
| title_short | Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells |
| title_sort | stress related hormone norepinephrine induces interleukin 6 expression in ges 1 cells |
| topic | Norepinephrine GES-1 cells Interleukin-6 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200101&lng=en&tlng=en |
| work_keys_str_mv | AT ryang stressrelatedhormonenorepinephrineinducesinterleukin6expressioninges1cells AT qlin stressrelatedhormonenorepinephrineinducesinterleukin6expressioninges1cells AT hbgao stressrelatedhormonenorepinephrineinducesinterleukin6expressioninges1cells AT pzhang stressrelatedhormonenorepinephrineinducesinterleukin6expressioninges1cells |