α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation
Summary: For almost two decades, clinicians have overlooked the diagnostic potential of CD34neg hematopoietic stem cells because of their limited homing capacity relative to CD34posHSCs when injected intravenously. This has contributed to the lack of appeal of using umbilical cord blood in HSC trans...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224001032 |
| _version_ | 1797336917595914240 |
|---|---|
| author | Asma S. Al-Amoodi Jing Kai Yanyan Li Jana S. Malki Abdullah Alghamdi Arwa Al-Ghuneim Alfonso Saera-Vila Satoshi Habuchi Jasmeen S. Merzaban |
| author_facet | Asma S. Al-Amoodi Jing Kai Yanyan Li Jana S. Malki Abdullah Alghamdi Arwa Al-Ghuneim Alfonso Saera-Vila Satoshi Habuchi Jasmeen S. Merzaban |
| author_sort | Asma S. Al-Amoodi |
| collection | DOAJ |
| description | Summary: For almost two decades, clinicians have overlooked the diagnostic potential of CD34neg hematopoietic stem cells because of their limited homing capacity relative to CD34posHSCs when injected intravenously. This has contributed to the lack of appeal of using umbilical cord blood in HSC transplantation because its stem cell count is lower than bone marrow. The present study reveals that the homing and engraftment of CD34negHSCs can be improved by adding the Sialyl Lewis X molecule via α1,3-fucosylation. This unlocks the potential for using this more primitive stem cell to treat blood disorders because our findings show CD34negHSCs have the capacity to regenerate cells in the bone marrow of mice for several months. Furthermore, our RNA sequencing analysis revealed that CD34negHSCs have unique adhesion pathways, downregulated in CD34posHSCs, that facilitate interaction with the bone marrow niche. Our findings suggest that CD34neg cells will best thrive when the HSC resides in its microenvironment. |
| first_indexed | 2024-03-08T09:01:52Z |
| format | Article |
| id | doaj.art-c801343de13e4f5c9345460b3766853c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T09:01:52Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c801343de13e4f5c9345460b3766853c2024-02-01T06:35:01ZengElsevieriScience2589-00422024-02-01272108882α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigationAsma S. Al-Amoodi0Jing Kai1Yanyan Li2Jana S. Malki3Abdullah Alghamdi4Arwa Al-Ghuneim5Alfonso Saera-Vila6Satoshi Habuchi7Jasmeen S. Merzaban8Bioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaSequentia Biotech SL, Barcelona, SpainBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBioscience Program, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia; KAUST Smart-Health Initiative, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia; Corresponding authorSummary: For almost two decades, clinicians have overlooked the diagnostic potential of CD34neg hematopoietic stem cells because of their limited homing capacity relative to CD34posHSCs when injected intravenously. This has contributed to the lack of appeal of using umbilical cord blood in HSC transplantation because its stem cell count is lower than bone marrow. The present study reveals that the homing and engraftment of CD34negHSCs can be improved by adding the Sialyl Lewis X molecule via α1,3-fucosylation. This unlocks the potential for using this more primitive stem cell to treat blood disorders because our findings show CD34negHSCs have the capacity to regenerate cells in the bone marrow of mice for several months. Furthermore, our RNA sequencing analysis revealed that CD34negHSCs have unique adhesion pathways, downregulated in CD34posHSCs, that facilitate interaction with the bone marrow niche. Our findings suggest that CD34neg cells will best thrive when the HSC resides in its microenvironment.http://www.sciencedirect.com/science/article/pii/S2589004224001032ImmunologyStem cells researchTranscriptomics |
| spellingShingle | Asma S. Al-Amoodi Jing Kai Yanyan Li Jana S. Malki Abdullah Alghamdi Arwa Al-Ghuneim Alfonso Saera-Vila Satoshi Habuchi Jasmeen S. Merzaban α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation iScience Immunology Stem cells research Transcriptomics |
| title | α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation |
| title_full | α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation |
| title_fullStr | α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation |
| title_full_unstemmed | α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation |
| title_short | α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation |
| title_sort | α1 3 fucosylation treatment improves cord blood cd34 negative hematopoietic stem cell navigation |
| topic | Immunology Stem cells research Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224001032 |
| work_keys_str_mv | AT asmasalamoodi a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT jingkai a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT yanyanli a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT janasmalki a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT abdullahalghamdi a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT arwaalghuneim a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT alfonsosaeravila a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT satoshihabuchi a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation AT jasmeensmerzaban a13fucosylationtreatmentimprovescordbloodcd34negativehematopoieticstemcellnavigation |