Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient sa...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366&lng=en&tlng=en |
| _version_ | 1818175813662539776 |
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| author | Rodolfo Patussi Correia Laiz Cameirão Bento Ana Carolina Apelle Bortolucci Anderson Marega Alexandre Andressa da Costa Vaz Daniela Schimidell Eduardo de Carvalho Pedro Fabricio Simões Perin Sonia Tsukasa Nozawa Cláudio Ernesto Albers Mendes Rodrigo de Souza Barroso Nydia Strachman Bacal |
| author_facet | Rodolfo Patussi Correia Laiz Cameirão Bento Ana Carolina Apelle Bortolucci Anderson Marega Alexandre Andressa da Costa Vaz Daniela Schimidell Eduardo de Carvalho Pedro Fabricio Simões Perin Sonia Tsukasa Nozawa Cláudio Ernesto Albers Mendes Rodrigo de Souza Barroso Nydia Strachman Bacal |
| author_sort | Rodolfo Patussi Correia |
| collection | DOAJ |
| description | ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones. |
| first_indexed | 2024-12-11T20:06:16Z |
| format | Article |
| id | doaj.art-c801e872fcbc4ddeb609a917f935c502 |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-11T20:06:16Z |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-c801e872fcbc4ddeb609a917f935c5022022-12-22T00:52:24ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-638514336637310.1590/S1679-45082016AO3641S1679-45082016000300366Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuriaRodolfo Patussi CorreiaLaiz Cameirão BentoAna Carolina Apelle BortolucciAnderson Marega AlexandreAndressa da Costa VazDaniela SchimidellEduardo de Carvalho PedroFabricio Simões PerinSonia Tsukasa NozawaCláudio Ernesto Albers MendesRodrigo de Souza BarrosoNydia Strachman BacalABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366&lng=en&tlng=enHemoglobinúria paroxística/diagnósticoCitometria de fluxo/métodosDesenvolvimento tecnológico |
| spellingShingle | Rodolfo Patussi Correia Laiz Cameirão Bento Ana Carolina Apelle Bortolucci Anderson Marega Alexandre Andressa da Costa Vaz Daniela Schimidell Eduardo de Carvalho Pedro Fabricio Simões Perin Sonia Tsukasa Nozawa Cláudio Ernesto Albers Mendes Rodrigo de Souza Barroso Nydia Strachman Bacal Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria Einstein (São Paulo) Hemoglobinúria paroxística/diagnóstico Citometria de fluxo/métodos Desenvolvimento tecnológico |
| title | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_full | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_fullStr | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_full_unstemmed | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_short | Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| title_sort | technical advances in flow cytometry based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria |
| topic | Hemoglobinúria paroxística/diagnóstico Citometria de fluxo/métodos Desenvolvimento tecnológico |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366&lng=en&tlng=en |
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