The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
Purpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=6;spage=480;epage=484;aulast=Mohanarangam |
| _version_ | 1818824176757112832 |
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| author | Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash |
| author_facet | Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash |
| author_sort | Suganthi Mohanarangam |
| collection | DOAJ |
| description | Purpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2nd, 5th, and 7th day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups. Results: The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 (P < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC. Conclusion: From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells. |
| first_indexed | 2024-12-18T23:51:43Z |
| format | Article |
| id | doaj.art-c8049955e0e94c98abe7ba6affd99dfe |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-12-18T23:51:43Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-c8049955e0e94c98abe7ba6affd99dfe2022-12-21T20:46:55ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2021-01-0125648048410.4103/jisp.jisp_396_20The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro studySuganthi MohanarangamDhayanand John VictorSangeetha SubramanianP SG PrakashPurpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2nd, 5th, and 7th day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups. Results: The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 (P < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC. Conclusion: From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells.http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=6;spage=480;epage=484;aulast=Mohanarangamabsorbable collagen spongenanocrystalline hydroxyapatite collagenosteoblast cellsperiostin |
| spellingShingle | Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study Journal of Indian Society of Periodontology absorbable collagen sponge nanocrystalline hydroxyapatite collagen osteoblast cells periostin |
| title | The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study |
| title_full | The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study |
| title_fullStr | The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study |
| title_full_unstemmed | The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study |
| title_short | The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study |
| title_sort | influence of periostin on osteoblastic adhesion and proliferation on collagen matrices an in vitro study |
| topic | absorbable collagen sponge nanocrystalline hydroxyapatite collagen osteoblast cells periostin |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=6;spage=480;epage=484;aulast=Mohanarangam |
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