Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vac...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019365338 |
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| author | M.S. Serena J.A. Cappuccio G.E. Metz C.G. Aspitia M. Dibárbora M. Gallo Calderón M.G. Echeverría |
| author_facet | M.S. Serena J.A. Cappuccio G.E. Metz C.G. Aspitia M. Dibárbora M. Gallo Calderón M.G. Echeverría |
| author_sort | M.S. Serena |
| collection | DOAJ |
| description | Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms. |
| first_indexed | 2024-12-22T20:17:58Z |
| format | Article |
| id | doaj.art-c8049e9842e84ce5ad67b6507594bc40 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T20:17:58Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c8049e9842e84ce5ad67b6507594bc402022-12-21T18:13:56ZengElsevierHeliyon2405-84402019-11-01511e02874Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in ArgentinaM.S. Serena0J.A. Cappuccio1G.E. Metz2C.G. Aspitia3M. Dibárbora4M. Gallo Calderón5M.G. Echeverría6Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calles 60 y 118, 1900, La Plata, Argentina; Investigadores del Consejo Nacional de Ciencia y Tecnología, CONICET, ArgentinaInvestigadores del Consejo Nacional de Ciencia y Tecnología, CONICET, Argentina; Grupo Sanidad Animal, EEA Marcos Juarez, INTA. Ruta 12 km 3 (2580) Marcos Juarez, Córdoba, ArgentinaLaboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calles 60 y 118, 1900, La Plata, Argentina; Investigadores del Consejo Nacional de Ciencia y Tecnología, CONICET, ArgentinaLaboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calles 60 y 118, 1900, La Plata, ArgentinaGrupo Sanidad Animal, EEA Marcos Juarez, INTA. Ruta 12 km 3 (2580) Marcos Juarez, Córdoba, ArgentinaInvestigadores del Consejo Nacional de Ciencia y Tecnología, CONICET, Argentina; Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo, 2468, C1440FFX, Ciudad Autónoma de Buenos Aires, ArgentinaLaboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calles 60 y 118, 1900, La Plata, Argentina; Investigadores del Consejo Nacional de Ciencia y Tecnología, CONICET, Argentina; Corresponding author.Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.http://www.sciencedirect.com/science/article/pii/S2405844019365338BiotechnologyImmunologyMicrobiologyMolecular biologyVeterinary medicinePorcine parvovirus |
| spellingShingle | M.S. Serena J.A. Cappuccio G.E. Metz C.G. Aspitia M. Dibárbora M. Gallo Calderón M.G. Echeverría Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina Heliyon Biotechnology Immunology Microbiology Molecular biology Veterinary medicine Porcine parvovirus |
| title | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_full | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_fullStr | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_full_unstemmed | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_short | Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina |
| title_sort | detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in argentina |
| topic | Biotechnology Immunology Microbiology Molecular biology Veterinary medicine Porcine parvovirus |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019365338 |
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