Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars
The Canada Western Red Spring wheat (Triticum aestivum L.) cultivars AAC Concord, AAC Prevail, CDC Hughes, Lillian, Glenlea, and elite line BW961 express a spectrum of resistance to leaf rust caused by Puccinia triticina Eriks. This study aimed to identify and map the leaf rust resistance of the cul...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1130768/full |
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| author | Firdissa E. Bokore Richard D. Cuthbert Ron E. Knox Colin W. Hiebert Curtis J. Pozniak Samia Berraies Yuefeng Ruan Brad Meyer Pierre Hucl Brent D. McCallum |
| author_facet | Firdissa E. Bokore Richard D. Cuthbert Ron E. Knox Colin W. Hiebert Curtis J. Pozniak Samia Berraies Yuefeng Ruan Brad Meyer Pierre Hucl Brent D. McCallum |
| author_sort | Firdissa E. Bokore |
| collection | DOAJ |
| description | The Canada Western Red Spring wheat (Triticum aestivum L.) cultivars AAC Concord, AAC Prevail, CDC Hughes, Lillian, Glenlea, and elite line BW961 express a spectrum of resistance to leaf rust caused by Puccinia triticina Eriks. This study aimed to identify and map the leaf rust resistance of the cultivars using three doubled haploid populations, AAC Prevail/BW961 (PB), CDC Hughes/AAC Concord (HC), and Lillian/Glenlea (LG). The populations were evaluated for seedling resistance in the greenhouse and adult plant disease response in the field at Morden, MB for 3 years and genotyped with the 90K wheat Infinium iSelect SNP array. Genetic maps were constructed to perform QTL analysis on the seedling and field leaf rust data. A total of three field leaf rust resistance QTL segregated in the PB population, five in the HC, and six in the LG population. In the PB population, BW961 contributed two QTL on chromosomes 2DS and 7DS, and AAC Prevail contributed a QTL on 4AL consistent across trials. Of the five QTL in HC, AAC Concord contributed two QTL on 4AL and 7AL consistent across trials and a QTL on 3DL.1 that provided seedling resistance only. CDC Hughes contributed two QTL on 1DS and 3DL.2. Lillian contributed four QTL significant in at least two of the three trials on 2BS, 4AL, 5AL, and 7AL, and Glenlea two QTL on 4BL and 7BL. The 1DS QTL from CDC Hughes, the 2DS from BW961, the 4AL from the AAC Prevail, AAC Concord, and Lillian, and the 7AL from AAC Concord and Lillian conferred seedling leaf rust resistance. The QTL on 4AL corresponded with Lr30 and was the same across cultivars AAC Prevail, AAC Concord, and Lillian, whereas the 7AL corresponding with LrCen was coincident between AAC Concord and Lillian. The 7DS and 2DS QTL in BW961 corresponded with Lr34 and Lr2a, respectively, and the 1DS QTL in CDC Hughes with Lr21. The QTL identified on 5AL could represent a novel gene. The results of this study will widen our knowledge of leaf rust resistance genes in Canadian wheat and their utilization in resistance breeding. |
| first_indexed | 2024-04-09T23:37:38Z |
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| language | English |
| last_indexed | 2024-04-09T23:37:38Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-c804df12238c4fa19a68c3d5793b08e42023-03-20T05:03:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11307681130768Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivarsFirdissa E. Bokore0Richard D. Cuthbert1Ron E. Knox2Colin W. Hiebert3Curtis J. Pozniak4Samia Berraies5Yuefeng Ruan6Brad Meyer7Pierre Hucl8Brent D. McCallum9Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaMorden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, CanadaDepartment of Plant Sciences, University of Saskatchewan, Saskatoon, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaDepartment of Plant Sciences, University of Saskatchewan, Saskatoon, SK, CanadaMorden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, CanadaThe Canada Western Red Spring wheat (Triticum aestivum L.) cultivars AAC Concord, AAC Prevail, CDC Hughes, Lillian, Glenlea, and elite line BW961 express a spectrum of resistance to leaf rust caused by Puccinia triticina Eriks. This study aimed to identify and map the leaf rust resistance of the cultivars using three doubled haploid populations, AAC Prevail/BW961 (PB), CDC Hughes/AAC Concord (HC), and Lillian/Glenlea (LG). The populations were evaluated for seedling resistance in the greenhouse and adult plant disease response in the field at Morden, MB for 3 years and genotyped with the 90K wheat Infinium iSelect SNP array. Genetic maps were constructed to perform QTL analysis on the seedling and field leaf rust data. A total of three field leaf rust resistance QTL segregated in the PB population, five in the HC, and six in the LG population. In the PB population, BW961 contributed two QTL on chromosomes 2DS and 7DS, and AAC Prevail contributed a QTL on 4AL consistent across trials. Of the five QTL in HC, AAC Concord contributed two QTL on 4AL and 7AL consistent across trials and a QTL on 3DL.1 that provided seedling resistance only. CDC Hughes contributed two QTL on 1DS and 3DL.2. Lillian contributed four QTL significant in at least two of the three trials on 2BS, 4AL, 5AL, and 7AL, and Glenlea two QTL on 4BL and 7BL. The 1DS QTL from CDC Hughes, the 2DS from BW961, the 4AL from the AAC Prevail, AAC Concord, and Lillian, and the 7AL from AAC Concord and Lillian conferred seedling leaf rust resistance. The QTL on 4AL corresponded with Lr30 and was the same across cultivars AAC Prevail, AAC Concord, and Lillian, whereas the 7AL corresponding with LrCen was coincident between AAC Concord and Lillian. The 7DS and 2DS QTL in BW961 corresponded with Lr34 and Lr2a, respectively, and the 1DS QTL in CDC Hughes with Lr21. The QTL identified on 5AL could represent a novel gene. The results of this study will widen our knowledge of leaf rust resistance genes in Canadian wheat and their utilization in resistance breeding.https://www.frontiersin.org/articles/10.3389/fpls.2023.1130768/fullTriticum aestivumPuccinia triticinaseedling resistanceQTLSNP markers |
| spellingShingle | Firdissa E. Bokore Richard D. Cuthbert Ron E. Knox Colin W. Hiebert Curtis J. Pozniak Samia Berraies Yuefeng Ruan Brad Meyer Pierre Hucl Brent D. McCallum Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars Frontiers in Plant Science Triticum aestivum Puccinia triticina seedling resistance QTL SNP markers |
| title | Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars |
| title_full | Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars |
| title_fullStr | Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars |
| title_full_unstemmed | Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars |
| title_short | Genetic mapping of leaf rust (Puccinia triticina Eriks) resistance genes in six Canadian spring wheat cultivars |
| title_sort | genetic mapping of leaf rust puccinia triticina eriks resistance genes in six canadian spring wheat cultivars |
| topic | Triticum aestivum Puccinia triticina seedling resistance QTL SNP markers |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1130768/full |
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