Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway
This study aims to investigate the wound-healing effectiveness of the phenolic compound, naringin, both in vitro and in vivo. Male mice were shaved on their dorsal skin under isoflurane, a biopsy punch was made in four symmetrical circular resection windows (6 mm) to induce a wound. These excision w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/5/1695 |
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| author | Jia-Hau Yen Wan-Ting Chio Chia-Ju Chuang Hsin-Ling Yang Sheng-Teng Huang |
| author_facet | Jia-Hau Yen Wan-Ting Chio Chia-Ju Chuang Hsin-Ling Yang Sheng-Teng Huang |
| author_sort | Jia-Hau Yen |
| collection | DOAJ |
| description | This study aims to investigate the wound-healing effectiveness of the phenolic compound, naringin, both in vitro and in vivo. Male mice were shaved on their dorsal skin under isoflurane, a biopsy punch was made in four symmetrical circular resection windows (6 mm) to induce a wound. These excision wounds were used to study the topical effects of naringin in terms of various biochemical, molecular, and histological parameters. We observed a significant recovery in the wound area. Increased levels of MMP-2, 9, 14, TIMP-2, VEGF-A, and VEGF-R1 were induced by naringin in the HaCaT cells. The time course experiments further revealed that levels of VEGF-A and B increased within 36 h; whereas levels of VEGF-C decreased. In line with this, VEGF-R3 levels, but not VEGF-R1 and 2 levels, increased soon after stimulation; although the increase subsided after 36 h. Additionally, naringin cream upregulated wound healing in vitro. The blockage of VEGF by Bevacizumab abolished the function of naringin cream on cell migration. Histological alterations in the wounded skin were restored by naringin cream, which accelerated wound healing via upregulated expression of growth factors (VEGF-A, B, and C and VEGF-R3), and thus increased MMP-2, 9, 14 expressions. |
| first_indexed | 2024-03-09T20:29:29Z |
| format | Article |
| id | doaj.art-c80535deecb141dd90d66c6d6b04fae9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:29:29Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c80535deecb141dd90d66c6d6b04fae92023-11-23T23:28:21ZengMDPI AGMolecules1420-30492022-03-01275169510.3390/molecules27051695Improved Wound Healing by Naringin Associated with MMP and the VEGF PathwayJia-Hau Yen0Wan-Ting Chio1Chia-Ju Chuang2Hsin-Ling Yang3Sheng-Teng Huang4Cancer Research Center for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung 40402, TaiwanCancer Research Center for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung 40402, TaiwanCancer Research Center for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung 40402, TaiwanInstitute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, TaiwanCancer Research Center for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung 40402, TaiwanThis study aims to investigate the wound-healing effectiveness of the phenolic compound, naringin, both in vitro and in vivo. Male mice were shaved on their dorsal skin under isoflurane, a biopsy punch was made in four symmetrical circular resection windows (6 mm) to induce a wound. These excision wounds were used to study the topical effects of naringin in terms of various biochemical, molecular, and histological parameters. We observed a significant recovery in the wound area. Increased levels of MMP-2, 9, 14, TIMP-2, VEGF-A, and VEGF-R1 were induced by naringin in the HaCaT cells. The time course experiments further revealed that levels of VEGF-A and B increased within 36 h; whereas levels of VEGF-C decreased. In line with this, VEGF-R3 levels, but not VEGF-R1 and 2 levels, increased soon after stimulation; although the increase subsided after 36 h. Additionally, naringin cream upregulated wound healing in vitro. The blockage of VEGF by Bevacizumab abolished the function of naringin cream on cell migration. Histological alterations in the wounded skin were restored by naringin cream, which accelerated wound healing via upregulated expression of growth factors (VEGF-A, B, and C and VEGF-R3), and thus increased MMP-2, 9, 14 expressions.https://www.mdpi.com/1420-3049/27/5/1695naringinwound healingMMPsVEGFsVEGFRs |
| spellingShingle | Jia-Hau Yen Wan-Ting Chio Chia-Ju Chuang Hsin-Ling Yang Sheng-Teng Huang Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway Molecules naringin wound healing MMPs VEGFs VEGFRs |
| title | Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway |
| title_full | Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway |
| title_fullStr | Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway |
| title_full_unstemmed | Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway |
| title_short | Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway |
| title_sort | improved wound healing by naringin associated with mmp and the vegf pathway |
| topic | naringin wound healing MMPs VEGFs VEGFRs |
| url | https://www.mdpi.com/1420-3049/27/5/1695 |
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