Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats
Abstract Background Eccrine sweat glands (ESGs) and hair follicles (HFs) are the prominent skin appendages regulating human body temperature. C57BL/6 mice and Sprague–Dawley (SD) rats are the most commonly used model animals for studying ESGs and HFs. Previous studies have shown the distribution of...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03416-z |
| _version_ | 1798038939868594176 |
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| author | Zixiu Chen Junhong Zhao Yongjing Yan Lei Zhang Lijie Du Xiang Liu Manxiu Cao Cangyu Wang Yue Tang Haihong Li |
| author_facet | Zixiu Chen Junhong Zhao Yongjing Yan Lei Zhang Lijie Du Xiang Liu Manxiu Cao Cangyu Wang Yue Tang Haihong Li |
| author_sort | Zixiu Chen |
| collection | DOAJ |
| description | Abstract Background Eccrine sweat glands (ESGs) and hair follicles (HFs) are the prominent skin appendages regulating human body temperature. C57BL/6 mice and Sprague–Dawley (SD) rats are the most commonly used model animals for studying ESGs and HFs. Previous studies have shown the distribution of ESGs and HFs in volar hindfeet of C57BL/6 mice, but there are few or no reports on the distribution of ESGs and HFs in volar forefeet of C57BL/6 mice and volar feet of SD rats. Here, we investigated the differential distribution and genetic determination of ESGs and HFs in the volar skin of C57BL/6 mice and SD rats through gross observation, iodine-starch sweat test, double staining with Nile Blue A and Oil Red O, hematoxylin and eosin (HE) staining, double immunofluorescence staining of LIM Homeobox 2 (LHX2)/Na+-K+-ATPase α1(NKA) or LHX2/Na+-K+-2Cl－ cotransporter 1 (NKCC1), and qRT-PCR detection of ESG-related gene Engrailed 1 (En1) and HF-related gene LHX2. Results The results showed ESGs but no HFs in the footpads of C57BL/6 mice and SD rats, both ESGs and HFs in the inter-footpads (IFPs) of C57BL/6 mice, and neither ESGs nor HFs in the IFPs of SD rats. The relative quantitative change in En1 was consistent with the differential distribution of ESGs, and the relative quantitative change of LHX2 was consistent with the differential distribution of HFs. Conclusion C57BL/6 mice and SD rats had their own characteristics in the distribution of ESGs and HFs in the volar skin, and researchers should choose mice or rats, and even forefeet or hindfeet as their research object according to different purposes. The study provides a basis for selection of optimal animal models to study development, wound healing and regeneration of skin appendages. |
| first_indexed | 2024-04-11T21:47:07Z |
| format | Article |
| id | doaj.art-c805c46e2a25498fa92e03962cbbcd57 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T21:47:07Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-c805c46e2a25498fa92e03962cbbcd572022-12-22T04:01:23ZengBMCBMC Veterinary Research1746-61482022-08-011811910.1186/s12917-022-03416-zDifferential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD ratsZixiu Chen0Junhong Zhao1Yongjing Yan2Lei Zhang3Lijie Du4Xiang Liu5Manxiu Cao6Cangyu Wang7Yue Tang8Haihong Li9Jinzhou Medical University Graduate Training Base, Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineDepartment of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineJinzhou Medical University Graduate Training Base, Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineMental Health Center, Taihe Hospital, Hubei University of MedicineDepartment of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineDepartment of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineJinzhou Medical University Graduate Training Base, Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineDepartment of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineDepartment of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineJinzhou Medical University Graduate Training Base, Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of MedicineAbstract Background Eccrine sweat glands (ESGs) and hair follicles (HFs) are the prominent skin appendages regulating human body temperature. C57BL/6 mice and Sprague–Dawley (SD) rats are the most commonly used model animals for studying ESGs and HFs. Previous studies have shown the distribution of ESGs and HFs in volar hindfeet of C57BL/6 mice, but there are few or no reports on the distribution of ESGs and HFs in volar forefeet of C57BL/6 mice and volar feet of SD rats. Here, we investigated the differential distribution and genetic determination of ESGs and HFs in the volar skin of C57BL/6 mice and SD rats through gross observation, iodine-starch sweat test, double staining with Nile Blue A and Oil Red O, hematoxylin and eosin (HE) staining, double immunofluorescence staining of LIM Homeobox 2 (LHX2)/Na+-K+-ATPase α1(NKA) or LHX2/Na+-K+-2Cl－ cotransporter 1 (NKCC1), and qRT-PCR detection of ESG-related gene Engrailed 1 (En1) and HF-related gene LHX2. Results The results showed ESGs but no HFs in the footpads of C57BL/6 mice and SD rats, both ESGs and HFs in the inter-footpads (IFPs) of C57BL/6 mice, and neither ESGs nor HFs in the IFPs of SD rats. The relative quantitative change in En1 was consistent with the differential distribution of ESGs, and the relative quantitative change of LHX2 was consistent with the differential distribution of HFs. Conclusion C57BL/6 mice and SD rats had their own characteristics in the distribution of ESGs and HFs in the volar skin, and researchers should choose mice or rats, and even forefeet or hindfeet as their research object according to different purposes. The study provides a basis for selection of optimal animal models to study development, wound healing and regeneration of skin appendages.https://doi.org/10.1186/s12917-022-03416-zEccrine sweat glandsHair folliclesFootpadsInter-footpadsVolar skinLHX2 |
| spellingShingle | Zixiu Chen Junhong Zhao Yongjing Yan Lei Zhang Lijie Du Xiang Liu Manxiu Cao Cangyu Wang Yue Tang Haihong Li Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats BMC Veterinary Research Eccrine sweat glands Hair follicles Footpads Inter-footpads Volar skin LHX2 |
| title | Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats |
| title_full | Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats |
| title_fullStr | Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats |
| title_full_unstemmed | Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats |
| title_short | Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats |
| title_sort | differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of c57bl 6 mice and sd rats |
| topic | Eccrine sweat glands Hair follicles Footpads Inter-footpads Volar skin LHX2 |
| url | https://doi.org/10.1186/s12917-022-03416-z |
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