Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6
Astragaloside IV, the active component of Astragalus membranaceus , exhibits diverse biological roles including the anti-tumor activity. In this study, we evaluated the chemosensitive role of astragaloside IV in non–small cell lung cancer cells. Cell Counting Kit-8 analysis was performed to determin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOS Press
2017-04-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317697555 |
| _version_ | 1818608354004566016 |
|---|---|
| author | Peng-Chen Dai De-Ling Liu Lei Zhang Jia Ye Qing Wang Hong-Wen Zhang Xiu-Hua Lin Guo-Xiang Lai |
| author_facet | Peng-Chen Dai De-Ling Liu Lei Zhang Jia Ye Qing Wang Hong-Wen Zhang Xiu-Hua Lin Guo-Xiang Lai |
| author_sort | Peng-Chen Dai |
| collection | DOAJ |
| description | Astragaloside IV, the active component of Astragalus membranaceus , exhibits diverse biological roles including the anti-tumor activity. In this study, we evaluated the chemosensitive role of astragaloside IV in non–small cell lung cancer cells. Cell Counting Kit-8 analysis was performed to determine cell viability. Real-time polymerase chain reaction and western blot were used to measure the messenger RNA and protein expression. Results showed that astragaloside IV treatment could suppress the proliferation of non–small cell lung cancer cells. In addition, combined treatment with astragaloside IV remarkably enhanced the chemosensitivity to gefitinib in three non–small cell lung cancer cell lines including NCI-H1299, HCC827, and A549. Furthermore, compared with gefitinib-treated cells, the messenger RNA expression of SIRT6 was obviously increased in non–small cell lung cancer cells treated with gefitinib combined with astragaloside IV. In addition, downregulation of SIRT6 was accomplished using small interference RNA technology. As a result, SIRT6 inhibition abolished the sensitization role of astragaloside IV in non–small cell lung cancer cells. Taken together, these data demonstrated that astragaloside IV sensitized tumor cells to gefitinib via regulation of SIRT6, suggesting that astragaloside IV may serve as potential therapeutic approach for lung cancer. |
| first_indexed | 2024-12-16T14:41:18Z |
| format | Article |
| id | doaj.art-c806b9fbea5e446eb133a679478a3361 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-16T14:41:18Z |
| publishDate | 2017-04-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-c806b9fbea5e446eb133a679478a33612022-12-21T22:27:55ZengIOS PressTumor Biology1423-03802017-04-013910.1177/1010428317697555Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6Peng-Chen DaiDe-Ling LiuLei ZhangJia YeQing WangHong-Wen ZhangXiu-Hua LinGuo-Xiang LaiAstragaloside IV, the active component of Astragalus membranaceus , exhibits diverse biological roles including the anti-tumor activity. In this study, we evaluated the chemosensitive role of astragaloside IV in non–small cell lung cancer cells. Cell Counting Kit-8 analysis was performed to determine cell viability. Real-time polymerase chain reaction and western blot were used to measure the messenger RNA and protein expression. Results showed that astragaloside IV treatment could suppress the proliferation of non–small cell lung cancer cells. In addition, combined treatment with astragaloside IV remarkably enhanced the chemosensitivity to gefitinib in three non–small cell lung cancer cell lines including NCI-H1299, HCC827, and A549. Furthermore, compared with gefitinib-treated cells, the messenger RNA expression of SIRT6 was obviously increased in non–small cell lung cancer cells treated with gefitinib combined with astragaloside IV. In addition, downregulation of SIRT6 was accomplished using small interference RNA technology. As a result, SIRT6 inhibition abolished the sensitization role of astragaloside IV in non–small cell lung cancer cells. Taken together, these data demonstrated that astragaloside IV sensitized tumor cells to gefitinib via regulation of SIRT6, suggesting that astragaloside IV may serve as potential therapeutic approach for lung cancer.https://doi.org/10.1177/1010428317697555 |
| spellingShingle | Peng-Chen Dai De-Ling Liu Lei Zhang Jia Ye Qing Wang Hong-Wen Zhang Xiu-Hua Lin Guo-Xiang Lai Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 Tumor Biology |
| title | Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 |
| title_full | Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 |
| title_fullStr | Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 |
| title_full_unstemmed | Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 |
| title_short | Astragaloside IV sensitizes non–small cell lung cancer cells to gefitinib potentially via regulation of SIRT6 |
| title_sort | astragaloside iv sensitizes non small cell lung cancer cells to gefitinib potentially via regulation of sirt6 |
| url | https://doi.org/10.1177/1010428317697555 |
| work_keys_str_mv | AT pengchendai astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT delingliu astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT leizhang astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT jiaye astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT qingwang astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT hongwenzhang astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT xiuhualin astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 AT guoxianglai astragalosideivsensitizesnonsmallcelllungcancercellstogefitinibpotentiallyviaregulationofsirt6 |