Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity
Cinnamomum stenophyllum (Meisn.) Vattimo-Gil (Lauraceae) is a native and vulnerable Brazilian species restricted to the Atlantic Forest. The leaf essential oil obtained by hydrodistillation was characterized for the first time by two-dimensional gas chromatography with time-of-flight mass spectromet...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Arabian Journal of Chemistry |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535220300162 |
| _version_ | 1811223021273219072 |
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| author | Fabiana L. Silva Raquel V.S. Silva Paola C. Branco Letícia V. Costa-Lotufo Cynthia Murakami Maria C.M. Young Débora A. Azevedo Paulo R.H. Moreno |
| author_facet | Fabiana L. Silva Raquel V.S. Silva Paola C. Branco Letícia V. Costa-Lotufo Cynthia Murakami Maria C.M. Young Débora A. Azevedo Paulo R.H. Moreno |
| author_sort | Fabiana L. Silva |
| collection | DOAJ |
| description | Cinnamomum stenophyllum (Meisn.) Vattimo-Gil (Lauraceae) is a native and vulnerable Brazilian species restricted to the Atlantic Forest. The leaf essential oil obtained by hydrodistillation was characterized for the first time by two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS). This analysis resulted in the tentatively identification of 80 compounds, showing the superior performance of this method in comparison to the seven compounds identified by GC–MS. The identified compounds included 8 ketones, 7 monoterpene hydrocarbons, 30 oxygenated monoterpenes, 4 sesquiterpene hydrocarbons and 23 oxygenated sesquiterpenes, showing that the C. stenophyllum oil contained mostly oxygenated mono and sesquiterpenes. The oil cytotoxicity was tested against two human cancer cell lines, colon adenocarcinoma (HCT-116) and breast cancer carcinoma (MCF-7), and the non-tumor retinal pigment epithelial cells (RPE) using the colorimetric MTT assay. Both cancer cell lines were sensible to leaf essential oil, with IC50 < 20 μg/mL (HCT, IC50 = 9.95 μg/mL and MCF-7, IC50 = 16.65 μg/mL), while there was no cytotoxicity against the non-tumor cells at tested concentrations (IC50 > 50 μg/mL), suggesting selectivity to cancer cells. The results showed that the C. stenophyllum leaf essential oil has a cytotoxic potential, presenting several compounds already known as biologically active against tumor cells. Keywords: Cinnamomum stenophyllum, Essential oil, GC×GC-TOFMS, Oxygenated mono- and sesquiterpenes, Cytotoxic activity |
| first_indexed | 2024-04-12T08:25:45Z |
| format | Article |
| id | doaj.art-c8088bb5e8044c83804cf57890b82cb8 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-12T08:25:45Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-c8088bb5e8044c83804cf57890b82cb82022-12-22T03:40:25ZengElsevierArabian Journal of Chemistry1878-53522020-04-0113449264935Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activityFabiana L. Silva0Raquel V.S. Silva1Paola C. Branco2Letícia V. Costa-Lotufo3Cynthia Murakami4Maria C.M. Young5Débora A. Azevedo6Paulo R.H. Moreno7Instituto de Ciências da Saúde, Universidade Paulista, 06542-001 Santana de Parnaíba, SP, Brazil; Instituto de Química, Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil; Corresponding authors at: Instituto de Ciências da Saúde, Universidade Paulista, 06542-001 Santana de Parnaíba, SP, Brazil (F.L. Silva). Laboratório de Química de Produtos Naturais, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748 Bloco 11 T, 05508-000 São Paulo, SP, Brazil (P. R. H. Moreno).Instituto de Química, Universidade Federal do Rio de Janeiro, 21941-598 Rio de Janeiro, RJ, BrazilInstituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, BrazilInstituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, BrazilInstituto de Botânica do Estado de São Paulo, 04301-902 São Paulo, SP, BrazilInstituto de Botânica do Estado de São Paulo, 04301-902 São Paulo, SP, BrazilInstituto de Química, Universidade Federal do Rio de Janeiro, 21941-598 Rio de Janeiro, RJ, BrazilInstituto de Química, Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil; Corresponding authors at: Instituto de Ciências da Saúde, Universidade Paulista, 06542-001 Santana de Parnaíba, SP, Brazil (F.L. Silva). Laboratório de Química de Produtos Naturais, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748 Bloco 11 T, 05508-000 São Paulo, SP, Brazil (P. R. H. Moreno).Cinnamomum stenophyllum (Meisn.) Vattimo-Gil (Lauraceae) is a native and vulnerable Brazilian species restricted to the Atlantic Forest. The leaf essential oil obtained by hydrodistillation was characterized for the first time by two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS). This analysis resulted in the tentatively identification of 80 compounds, showing the superior performance of this method in comparison to the seven compounds identified by GC–MS. The identified compounds included 8 ketones, 7 monoterpene hydrocarbons, 30 oxygenated monoterpenes, 4 sesquiterpene hydrocarbons and 23 oxygenated sesquiterpenes, showing that the C. stenophyllum oil contained mostly oxygenated mono and sesquiterpenes. The oil cytotoxicity was tested against two human cancer cell lines, colon adenocarcinoma (HCT-116) and breast cancer carcinoma (MCF-7), and the non-tumor retinal pigment epithelial cells (RPE) using the colorimetric MTT assay. Both cancer cell lines were sensible to leaf essential oil, with IC50 < 20 μg/mL (HCT, IC50 = 9.95 μg/mL and MCF-7, IC50 = 16.65 μg/mL), while there was no cytotoxicity against the non-tumor cells at tested concentrations (IC50 > 50 μg/mL), suggesting selectivity to cancer cells. The results showed that the C. stenophyllum leaf essential oil has a cytotoxic potential, presenting several compounds already known as biologically active against tumor cells. Keywords: Cinnamomum stenophyllum, Essential oil, GC×GC-TOFMS, Oxygenated mono- and sesquiterpenes, Cytotoxic activityhttp://www.sciencedirect.com/science/article/pii/S1878535220300162 |
| spellingShingle | Fabiana L. Silva Raquel V.S. Silva Paola C. Branco Letícia V. Costa-Lotufo Cynthia Murakami Maria C.M. Young Débora A. Azevedo Paulo R.H. Moreno Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity Arabian Journal of Chemistry |
| title | Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity |
| title_full | Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity |
| title_fullStr | Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity |
| title_full_unstemmed | Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity |
| title_short | Chemical composition of the Brazilian native Cinnamomum stenophyllum (Meisn.) Vattimo-Gil essential oil by GC-qMS and GC × GC-TOFMS, and its cytotoxic activity |
| title_sort | chemical composition of the brazilian native cinnamomum stenophyllum meisn vattimo gil essential oil by gc qms and gc gc tofms and its cytotoxic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1878535220300162 |
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