Proteome data of neuroblastoma cells overexpressing Neuroglobin
In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this que...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922000555 |
| _version_ | 1823938590083645440 |
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| author | Michele Costanzo Marianna Caterino Illari Salvatori Valeria Manganelli Alberto Ferri Roberta Misasi Margherita Ruoppolo |
| author_facet | Michele Costanzo Marianna Caterino Illari Salvatori Valeria Manganelli Alberto Ferri Roberta Misasi Margherita Ruoppolo |
| author_sort | Michele Costanzo |
| collection | DOAJ |
| description | In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012. |
| first_indexed | 2024-12-17T02:47:39Z |
| format | Article |
| id | doaj.art-c811c0cd1c1b4a369f9baaf61c24c0f9 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-17T02:47:39Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-c811c0cd1c1b4a369f9baaf61c24c0f92022-12-21T22:06:31ZengElsevierData in Brief2352-34092022-04-0141107843Proteome data of neuroblastoma cells overexpressing NeuroglobinMichele Costanzo0Marianna Caterino1Illari Salvatori2Valeria Manganelli3Alberto Ferri4Roberta Misasi5Margherita Ruoppolo6Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy; CEINGE-Biotecnologie Avanzate s.c.ar.l., Naples 80145, Italy; Corresponding author at: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy.Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy; CEINGE-Biotecnologie Avanzate s.c.ar.l., Naples 80145, ItalyFondazione Santa Lucia IRCCS, c/o CERC, Rome 00143, ItalyDepartment of Experimental Medicine, ''Sapienza'' University of Rome, Rome 00161, ItalyFondazione Santa Lucia IRCCS, c/o CERC, Rome 00143, ItalyDepartment of Experimental Medicine, ''Sapienza'' University of Rome, Rome 00161, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples 80131, Italy; CEINGE-Biotecnologie Avanzate s.c.ar.l., Naples 80145, ItalyIn this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012.http://www.sciencedirect.com/science/article/pii/S2352340922000555ProteomicsNeuroglobinLabel-free quantificationLC-MS/MSS-TrapMaxQuant |
| spellingShingle | Michele Costanzo Marianna Caterino Illari Salvatori Valeria Manganelli Alberto Ferri Roberta Misasi Margherita Ruoppolo Proteome data of neuroblastoma cells overexpressing Neuroglobin Data in Brief Proteomics Neuroglobin Label-free quantification LC-MS/MS S-Trap MaxQuant |
| title | Proteome data of neuroblastoma cells overexpressing Neuroglobin |
| title_full | Proteome data of neuroblastoma cells overexpressing Neuroglobin |
| title_fullStr | Proteome data of neuroblastoma cells overexpressing Neuroglobin |
| title_full_unstemmed | Proteome data of neuroblastoma cells overexpressing Neuroglobin |
| title_short | Proteome data of neuroblastoma cells overexpressing Neuroglobin |
| title_sort | proteome data of neuroblastoma cells overexpressing neuroglobin |
| topic | Proteomics Neuroglobin Label-free quantification LC-MS/MS S-Trap MaxQuant |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922000555 |
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