Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds
Background: Tissue engineering of the annulus fibrosus (AF) shows promise as a treatment for patients with degenerative disc disease (DDD). However, it remains challenging due to the intrinsic heterogeneity of AF tissue. Fabrication of scaffolds recapitulating the specific cellular, componential, an...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Orthopaedic Translation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X20300127 |
| _version_ | 1818647864334614528 |
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| author | Pinghui Zhou Genglei Chu Zhangqin Yuan Huan Wang Weidong Zhang Yingji Mao Xuesong Zhu Weiguo Chen Huilin Yang Bin Li |
| author_facet | Pinghui Zhou Genglei Chu Zhangqin Yuan Huan Wang Weidong Zhang Yingji Mao Xuesong Zhu Weiguo Chen Huilin Yang Bin Li |
| author_sort | Pinghui Zhou |
| collection | DOAJ |
| description | Background: Tissue engineering of the annulus fibrosus (AF) shows promise as a treatment for patients with degenerative disc disease (DDD). However, it remains challenging due to the intrinsic heterogeneity of AF tissue. Fabrication of scaffolds recapitulating the specific cellular, componential, and microstructural features of AF, therefore, is critical to successful AF tissue regeneration. Methods: Poly-L-lactic acid (PLLA) fibrous scaffolds with various fiber diameters and orientation were prepared to mimic the microstructural characteristics of AF tissue using electrospinning technique. AF-derived stem cells (AFSCs) were cultured on the PLLA fibrous scaffolds for 7 days. Results: The morphology of AFSCs significantly varied when cultured on the scaffolds with various fiber diameters and orientation. AFSCs were nearly round on scaffolds with small fibers. However, they became spindle-shaped on scaffolds with large fibers. Meanwhile, upregulated expression of collagen-I gene happened in cells cultured on scaffolds with large fibers, while enhanced expression of collagen-II and aggrecan genes was seen on scaffolds with small fibers. The production of related proteins also showed similar trends. Further, culturing AFSCs on a heterogeneous scaffold by overlaying membranes with different fiber sizes led to the formation of a hierarchical structure approximating native AF tissue. Conclusion: Findings from this study demonstrate that fibrous scaffolds with different fiber sizes effectively promoted the differentiation of AFSCs into specific cells similar to the types of cells at various AF zones. It also provides a valuable reference for regulation of cell differentiation and fabrication of engineered tissues with complex hierarchical structures using the physical cues of scaffolds. The translational potential of this article: Effective AF repair is an essential need for treating degenerative disc disease. Tissue engineering is a promising approach to achieving tissue regeneration and restoring normal functions of tissues. By mimicking the key structural features of native AF tissue, including fiber size and alignment, this study deciphered the effect of scaffold materials on the cell differentiation and extracellular matrix deposition, which provides a solid basis for designing new strategies toward more effective AF repair and regeneration. |
| first_indexed | 2024-12-17T01:09:18Z |
| format | Article |
| id | doaj.art-c8125c27506a41189e9646c71057d3fa |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-12-17T01:09:18Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-c8125c27506a41189e9646c71057d3fa2022-12-21T22:09:11ZengElsevierJournal of Orthopaedic Translation2214-031X2021-01-0126171180Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffoldsPinghui Zhou0Genglei Chu1Zhangqin Yuan2Huan Wang3Weidong Zhang4Yingji Mao5Xuesong Zhu6Weiguo Chen7Huilin Yang8Bin Li9Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China; Anhui Province Key Laboratory of Tissue Transplantation, School of Life Sciences, Bengbu Medical College, Bengbu, Anhui, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, ChinaAnhui Province Key Laboratory of Tissue Transplantation, School of Life Sciences, Bengbu Medical College, Bengbu, Anhui, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China; Corresponding author. Soochow University (South Campus), 708 Renmin Road, Rm 308 Bldg 1, Suzhou, Jiangsu, 215007, China.Departments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China; Corresponding author. Soochow University (South Campus), 708 Renmin Road, Rm 308 Bldg 1, Suzhou, Jiangsu, 215007, China.Departments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, ChinaDepartments of Orthopaedic Surgery and Urology, The First Affiliated Hospital, Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China; Corresponding author. Soochow University (South Campus), 708 Renmin Road, Rm 308 Bldg 1, Suzhou, Jiangsu, 215007, China.Background: Tissue engineering of the annulus fibrosus (AF) shows promise as a treatment for patients with degenerative disc disease (DDD). However, it remains challenging due to the intrinsic heterogeneity of AF tissue. Fabrication of scaffolds recapitulating the specific cellular, componential, and microstructural features of AF, therefore, is critical to successful AF tissue regeneration. Methods: Poly-L-lactic acid (PLLA) fibrous scaffolds with various fiber diameters and orientation were prepared to mimic the microstructural characteristics of AF tissue using electrospinning technique. AF-derived stem cells (AFSCs) were cultured on the PLLA fibrous scaffolds for 7 days. Results: The morphology of AFSCs significantly varied when cultured on the scaffolds with various fiber diameters and orientation. AFSCs were nearly round on scaffolds with small fibers. However, they became spindle-shaped on scaffolds with large fibers. Meanwhile, upregulated expression of collagen-I gene happened in cells cultured on scaffolds with large fibers, while enhanced expression of collagen-II and aggrecan genes was seen on scaffolds with small fibers. The production of related proteins also showed similar trends. Further, culturing AFSCs on a heterogeneous scaffold by overlaying membranes with different fiber sizes led to the formation of a hierarchical structure approximating native AF tissue. Conclusion: Findings from this study demonstrate that fibrous scaffolds with different fiber sizes effectively promoted the differentiation of AFSCs into specific cells similar to the types of cells at various AF zones. It also provides a valuable reference for regulation of cell differentiation and fabrication of engineered tissues with complex hierarchical structures using the physical cues of scaffolds. The translational potential of this article: Effective AF repair is an essential need for treating degenerative disc disease. Tissue engineering is a promising approach to achieving tissue regeneration and restoring normal functions of tissues. By mimicking the key structural features of native AF tissue, including fiber size and alignment, this study deciphered the effect of scaffold materials on the cell differentiation and extracellular matrix deposition, which provides a solid basis for designing new strategies toward more effective AF repair and regeneration.http://www.sciencedirect.com/science/article/pii/S2214031X20300127Annulus fibrosusAnnulus fibrosus-derived stem cellsCell differentiationDegenerative disc diseaseFiber size |
| spellingShingle | Pinghui Zhou Genglei Chu Zhangqin Yuan Huan Wang Weidong Zhang Yingji Mao Xuesong Zhu Weiguo Chen Huilin Yang Bin Li Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds Journal of Orthopaedic Translation Annulus fibrosus Annulus fibrosus-derived stem cells Cell differentiation Degenerative disc disease Fiber size |
| title | Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds |
| title_full | Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds |
| title_fullStr | Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds |
| title_full_unstemmed | Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds |
| title_short | Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds |
| title_sort | regulation of differentiation of annulus fibrosus derived stem cells using heterogeneous electrospun fibrous scaffolds |
| topic | Annulus fibrosus Annulus fibrosus-derived stem cells Cell differentiation Degenerative disc disease Fiber size |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X20300127 |
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