Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring

In this study, we hypothesized that blunting of the natriuresis response to intracerebroventricularly (ICV) microinjected adrenergic agonists is involved in the development of hypertension in maternal low-protein intake (LP) offspring. A stainless steel cannula was stereotaxically implanted into the...

Full description

Bibliographic Details
Main Authors: Bárbara Vaccari Cardoso, Augusto Henrique Custódio, Patrícia Aline Boer, José Antonio Rocha Gontijo
Format: Article
Language:English
Published: The Company of Biologists 2019-04-01
Series:Biology Open
Subjects:
Online Access:http://bio.biologists.org/content/8/4/bio038562
_version_ 1818672665069617152
author Bárbara Vaccari Cardoso
Augusto Henrique Custódio
Patrícia Aline Boer
José Antonio Rocha Gontijo
author_facet Bárbara Vaccari Cardoso
Augusto Henrique Custódio
Patrícia Aline Boer
José Antonio Rocha Gontijo
author_sort Bárbara Vaccari Cardoso
collection DOAJ
description In this study, we hypothesized that blunting of the natriuresis response to intracerebroventricularly (ICV) microinjected adrenergic agonists is involved in the development of hypertension in maternal low-protein intake (LP) offspring. A stainless steel cannula was stereotaxically implanted into the right lateral ventricle (LV), then we evaluated the ICV administration of adrenergic agonists at increasing concentrations, and of α1 and α2-adrenoceptor antagonists on blood pressure and urinary sodium handling in LP offspring relative to an age-matched normal-protein intake (NP) group. We confirmed that epinephrine (Epi) microinjected into the LV of conscious NP rats leads to enhanced natriuresis followed by a reduction in arterial pressure. This response is associated with increased proximal and post-proximal sodium excretion accompanied by an unchanged glomerular filtration rate. The current study showed, in both NP and LP offspring, that the natriuretic effect of Epi injection into the LV was abolished by prior local microinjection of an α1-adrenoceptor antagonist (prazosin). Conversely, LV α2-adrenoceptor antagonist (yohimbine) administration potentiated the action of Epi. The LV yohimbine pretreatment normalized urinary sodium excretion and reduced the blood pressure in LP compared with age-matched NP offspring. These are, as far as we are aware, the first results showing the role of central adrenergic receptors’ interaction on hypertension pathogenesis in maternal LP fetal-programming offspring. This study also provides good evidence for the existence of central nervous system adrenergic mechanisms consisting of α1 and α2-adrenoceptors, which work reciprocally on the control of renal sodium excretion and blood pressure. Although the precise mechanism of the different natriuretic response of NP and LP rats is still uncertain, these results lead us to speculate that inappropriate neural adrenergic pathways might have significant effects on tubule sodium transport, resulting in the inability of the kidneys to control hydrosaline balance and, consequently, an increase in blood pressure.
first_indexed 2024-12-17T07:43:30Z
format Article
id doaj.art-c81356b1f2514b3e9488373617fa33a8
institution Directory Open Access Journal
issn 2046-6390
language English
last_indexed 2024-12-17T07:43:30Z
publishDate 2019-04-01
publisher The Company of Biologists
record_format Article
series Biology Open
spelling doaj.art-c81356b1f2514b3e9488373617fa33a82022-12-21T21:58:05ZengThe Company of BiologistsBiology Open2046-63902019-04-018410.1242/bio.038562038562Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspringBárbara Vaccari Cardoso0Augusto Henrique Custódio1Patrícia Aline Boer2José Antonio Rocha Gontijo3 Fetal Programming Laboratory and Hydroelectrolyte Metabolism Laboratory, Nucleus of Medicine and Experimental Surgery, Department of Internal Medicine, Faculty of Medical Sciences at State University of Campinas, Campinas 13083-894, São Paulo, Brazil Fetal Programming Laboratory and Hydroelectrolyte Metabolism Laboratory, Nucleus of Medicine and Experimental Surgery, Department of Internal Medicine, Faculty of Medical Sciences at State University of Campinas, Campinas 13083-894, São Paulo, Brazil Fetal Programming Laboratory and Hydroelectrolyte Metabolism Laboratory, Nucleus of Medicine and Experimental Surgery, Department of Internal Medicine, Faculty of Medical Sciences at State University of Campinas, Campinas 13083-894, São Paulo, Brazil Fetal Programming Laboratory and Hydroelectrolyte Metabolism Laboratory, Nucleus of Medicine and Experimental Surgery, Department of Internal Medicine, Faculty of Medical Sciences at State University of Campinas, Campinas 13083-894, São Paulo, Brazil In this study, we hypothesized that blunting of the natriuresis response to intracerebroventricularly (ICV) microinjected adrenergic agonists is involved in the development of hypertension in maternal low-protein intake (LP) offspring. A stainless steel cannula was stereotaxically implanted into the right lateral ventricle (LV), then we evaluated the ICV administration of adrenergic agonists at increasing concentrations, and of α1 and α2-adrenoceptor antagonists on blood pressure and urinary sodium handling in LP offspring relative to an age-matched normal-protein intake (NP) group. We confirmed that epinephrine (Epi) microinjected into the LV of conscious NP rats leads to enhanced natriuresis followed by a reduction in arterial pressure. This response is associated with increased proximal and post-proximal sodium excretion accompanied by an unchanged glomerular filtration rate. The current study showed, in both NP and LP offspring, that the natriuretic effect of Epi injection into the LV was abolished by prior local microinjection of an α1-adrenoceptor antagonist (prazosin). Conversely, LV α2-adrenoceptor antagonist (yohimbine) administration potentiated the action of Epi. The LV yohimbine pretreatment normalized urinary sodium excretion and reduced the blood pressure in LP compared with age-matched NP offspring. These are, as far as we are aware, the first results showing the role of central adrenergic receptors’ interaction on hypertension pathogenesis in maternal LP fetal-programming offspring. This study also provides good evidence for the existence of central nervous system adrenergic mechanisms consisting of α1 and α2-adrenoceptors, which work reciprocally on the control of renal sodium excretion and blood pressure. Although the precise mechanism of the different natriuretic response of NP and LP rats is still uncertain, these results lead us to speculate that inappropriate neural adrenergic pathways might have significant effects on tubule sodium transport, resulting in the inability of the kidneys to control hydrosaline balance and, consequently, an increase in blood pressure.http://bio.biologists.org/content/8/4/bio038562Fetal programmingCentral nervous systemRenal functionMaternal protein-restrictionNatriuresisArterial hypertensionAdrenergic system
spellingShingle Bárbara Vaccari Cardoso
Augusto Henrique Custódio
Patrícia Aline Boer
José Antonio Rocha Gontijo
Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
Biology Open
Fetal programming
Central nervous system
Renal function
Maternal protein-restriction
Natriuresis
Arterial hypertension
Adrenergic system
title Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
title_full Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
title_fullStr Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
title_full_unstemmed Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
title_short Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein-restricted male adult rat offspring
title_sort effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestational protein restricted male adult rat offspring
topic Fetal programming
Central nervous system
Renal function
Maternal protein-restriction
Natriuresis
Arterial hypertension
Adrenergic system
url http://bio.biologists.org/content/8/4/bio038562
work_keys_str_mv AT barbaravaccaricardoso effectofintracerebroventricularepinephrinemicroinjectiononbloodpressureandurinarysodiumhandlingingestationalproteinrestrictedmaleadultratoffspring
AT augustohenriquecustodio effectofintracerebroventricularepinephrinemicroinjectiononbloodpressureandurinarysodiumhandlingingestationalproteinrestrictedmaleadultratoffspring
AT patriciaalineboer effectofintracerebroventricularepinephrinemicroinjectiononbloodpressureandurinarysodiumhandlingingestationalproteinrestrictedmaleadultratoffspring
AT joseantoniorochagontijo effectofintracerebroventricularepinephrinemicroinjectiononbloodpressureandurinarysodiumhandlingingestationalproteinrestrictedmaleadultratoffspring