Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry
Background: Peritoneal lavage (PL) during laparotomy is a treatment option for preterm bacterial peritonitis; however, its efficacy remains controversial. We investigated the effects of PL temperature during laparotomy on survival in a cecal slurry (CS)-induced preterm peritonitis mouse model. Metho...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of Pediatric Surgery Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S294971162300014X |
| _version_ | 1797788346993344512 |
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| author | Shohei Yoshimura Yuichi Okata Yoshitomo Samejima Harunori Miyauchi Masafumi Saito Shigeaki Inoue Kazumichi Fujioka Serena Iwabuchi Yasuyuki Kameoka Aya Watanabe Kotaro Uemura Yuichiro Tomioka Yuko Bitoh |
| author_facet | Shohei Yoshimura Yuichi Okata Yoshitomo Samejima Harunori Miyauchi Masafumi Saito Shigeaki Inoue Kazumichi Fujioka Serena Iwabuchi Yasuyuki Kameoka Aya Watanabe Kotaro Uemura Yuichiro Tomioka Yuko Bitoh |
| author_sort | Shohei Yoshimura |
| collection | DOAJ |
| description | Background: Peritoneal lavage (PL) during laparotomy is a treatment option for preterm bacterial peritonitis; however, its efficacy remains controversial. We investigated the effects of PL temperature during laparotomy on survival in a cecal slurry (CS)-induced preterm peritonitis mouse model. Methods: Four-day-old pups that were intraperitoneally administered 1.8 mg/g CS solution underwent laparotomy through a 5-mm transverse incision 2 h after CS injection (sham group). An additional PL was established during laparotomy with 500 µl of 25°C, 35°C, and 45°C sterile saline, respectively (PL25, PL35, and PL45 groups). Postoperative 7-day survival, body temperature, and plasma and ascites cytokine concentrations were evaluated for each group. Results: Postoperative 7-day survival rates in the sham, PL25, PL35, and PL45 groups were 52.9%, 56.3%, 33.3%, and 18.2% (p=0.097), respectively. Median BT changes between before and after surgery were −1.85°C, −2.30°C, −3.40°C, and −1.80°C for each group (p=0.0065). The median ascites MCP-1 and IL-6 concentrations (pg/ml) at postoperative 2 h were 32,432, 16,244, 6279, and 5100 (p=0.049) and 22,346, 11,537, 5519, and 4535 (p=0.076), and plasma MCP-1 and IL-6 concentrations (pg/ml) were 68,687, 16,327, 9277, and 1868 (p=0.084) and 112,290, 14,415, 11,403, and 4113 (p=0.123) for each group, respectively. Conclusion: Postoperative 7-day survivals were slightly improved by PL with 25°C sterile saline, however decreased by increasing PL temperatures to 35°C and 45°C in a CS-induced preterm peritonitis mouse model. As PL temperature was increased, both plasma and ascites MCP-1 and IL-6 concentrations tended to be suppressed, suggesting to decrease postoperative survivals. |
| first_indexed | 2024-03-13T01:35:16Z |
| format | Article |
| id | doaj.art-c8144beb5bc24259bb6e5d69327dc45b |
| institution | Directory Open Access Journal |
| issn | 2949-7116 |
| language | English |
| last_indexed | 2024-03-13T01:35:16Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pediatric Surgery Open |
| spelling | doaj.art-c8144beb5bc24259bb6e5d69327dc45b2023-07-04T05:12:30ZengElsevierJournal of Pediatric Surgery Open2949-71162023-07-012100016Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurryShohei Yoshimura0Yuichi Okata1Yoshitomo Samejima2Harunori Miyauchi3Masafumi Saito4Shigeaki Inoue5Kazumichi Fujioka6Serena Iwabuchi7Yasuyuki Kameoka8Aya Watanabe9Kotaro Uemura10Yuichiro Tomioka11Yuko Bitoh12Division of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan; Corresponding author.Division of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDepartment of Pediatric Surgery, Kobe Children's Hospital, Kobe, JapanDepartment of Disaster and Emergency, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, JapanDepartment of Disaster and Emergency, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, JapanDepartment of Pediatrics, Kobe University Graduate School of Medicine, Kobe, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanBackground: Peritoneal lavage (PL) during laparotomy is a treatment option for preterm bacterial peritonitis; however, its efficacy remains controversial. We investigated the effects of PL temperature during laparotomy on survival in a cecal slurry (CS)-induced preterm peritonitis mouse model. Methods: Four-day-old pups that were intraperitoneally administered 1.8 mg/g CS solution underwent laparotomy through a 5-mm transverse incision 2 h after CS injection (sham group). An additional PL was established during laparotomy with 500 µl of 25°C, 35°C, and 45°C sterile saline, respectively (PL25, PL35, and PL45 groups). Postoperative 7-day survival, body temperature, and plasma and ascites cytokine concentrations were evaluated for each group. Results: Postoperative 7-day survival rates in the sham, PL25, PL35, and PL45 groups were 52.9%, 56.3%, 33.3%, and 18.2% (p=0.097), respectively. Median BT changes between before and after surgery were −1.85°C, −2.30°C, −3.40°C, and −1.80°C for each group (p=0.0065). The median ascites MCP-1 and IL-6 concentrations (pg/ml) at postoperative 2 h were 32,432, 16,244, 6279, and 5100 (p=0.049) and 22,346, 11,537, 5519, and 4535 (p=0.076), and plasma MCP-1 and IL-6 concentrations (pg/ml) were 68,687, 16,327, 9277, and 1868 (p=0.084) and 112,290, 14,415, 11,403, and 4113 (p=0.123) for each group, respectively. Conclusion: Postoperative 7-day survivals were slightly improved by PL with 25°C sterile saline, however decreased by increasing PL temperatures to 35°C and 45°C in a CS-induced preterm peritonitis mouse model. As PL temperature was increased, both plasma and ascites MCP-1 and IL-6 concentrations tended to be suppressed, suggesting to decrease postoperative survivals.http://www.sciencedirect.com/science/article/pii/S294971162300014XPreterm bowel perforationBacterial peritonitisLaparotomyPeritoneal lavageCecal slurryMouse model |
| spellingShingle | Shohei Yoshimura Yuichi Okata Yoshitomo Samejima Harunori Miyauchi Masafumi Saito Shigeaki Inoue Kazumichi Fujioka Serena Iwabuchi Yasuyuki Kameoka Aya Watanabe Kotaro Uemura Yuichiro Tomioka Yuko Bitoh Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry Journal of Pediatric Surgery Open Preterm bowel perforation Bacterial peritonitis Laparotomy Peritoneal lavage Cecal slurry Mouse model |
| title | Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| title_full | Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| title_fullStr | Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| title_full_unstemmed | Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| title_short | Impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| title_sort | impact of peritoneal lavage temperature during laparotomy in a preterm peritonitis mouse model using cecal slurry |
| topic | Preterm bowel perforation Bacterial peritonitis Laparotomy Peritoneal lavage Cecal slurry Mouse model |
| url | http://www.sciencedirect.com/science/article/pii/S294971162300014X |
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