Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses
Infection can hinder the process of wound healing, so it is important to begin antibacterial treatment quickly after a wound forms. Plasma activated water (PAW) can inactivate a variety of common wound infection bacteria. In this study, we compared the effects of PAW prepared with portable surface d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Biophysica |
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| Online Access: | https://www.mdpi.com/2673-4125/1/3/22 |
| _version_ | 1797520071252246528 |
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| author | Shuai Wang Dehui Xu Miao Qi Bing Li Sansan Peng Qiaosong Li Hao Zhang Dingxin Liu |
| author_facet | Shuai Wang Dehui Xu Miao Qi Bing Li Sansan Peng Qiaosong Li Hao Zhang Dingxin Liu |
| author_sort | Shuai Wang |
| collection | DOAJ |
| description | Infection can hinder the process of wound healing, so it is important to begin antibacterial treatment quickly after a wound forms. Plasma activated water (PAW) can inactivate a variety of common wound infection bacteria. In this study, we compared the effects of PAW prepared with portable surface discharge plasma equipment and medical alcohol on wound healing in a mouse full-thickness skin wound model. The effectiveness of wound healing processes in mice was ranked accordingly: PAW treatment group > medical alcohol treatment group > control group. In order to further understand the mechanism of PAW in promoting wound healing, we tested the expression levels of the pro-inflammatory factors interleukin (IL)-1β and IL-6, the anti-inflammatory factor IL-10, and vascular endothelial growth factor (VEGF). The results showed that PAW promoted the release of pro-inflammatory factors and anti-inflammatory factors from the wounds in mice, which allowed the mice in the treatment group to transition out of the inflammatory period early and enter the next stage of wound healing. The expression level of VEGF in the wounds of mice in the PAW treatment group was higher, which indicates that the microvessels around the wound in the PAW treatment group proliferated faster, and thus the wound healed faster. PAW biosafety experiments showed that PAW did not significantly affect the appearance, morphology, or tissue structure of internal organs, or blood biochemical indicators in mice. In general, PAW prepared via portable devices is expected to become more widely used given its convenience, affordability, and lack of side effects in promoting wound healing. |
| first_indexed | 2024-03-10T07:51:38Z |
| format | Article |
| id | doaj.art-c816703c0baa4304bad78f98700828fa |
| institution | Directory Open Access Journal |
| issn | 2673-4125 |
| language | English |
| last_indexed | 2024-03-10T07:51:38Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biophysica |
| spelling | doaj.art-c816703c0baa4304bad78f98700828fa2023-11-22T12:12:05ZengMDPI AGBiophysica2673-41252021-07-011329731010.3390/biophysica1030022Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory ResponsesShuai Wang0Dehui Xu1Miao Qi2Bing Li3Sansan Peng4Qiaosong Li5Hao Zhang6Dingxin Liu7Centre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaCentre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaThe School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaThe School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, ChinaCentre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaCentre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaCentre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaCentre for Plasma Biomedicine, State Key Laboratory of Electrical Insulation and Power Equipment, Xi’an Jiaotong University, Xi’an 710049, ChinaInfection can hinder the process of wound healing, so it is important to begin antibacterial treatment quickly after a wound forms. Plasma activated water (PAW) can inactivate a variety of common wound infection bacteria. In this study, we compared the effects of PAW prepared with portable surface discharge plasma equipment and medical alcohol on wound healing in a mouse full-thickness skin wound model. The effectiveness of wound healing processes in mice was ranked accordingly: PAW treatment group > medical alcohol treatment group > control group. In order to further understand the mechanism of PAW in promoting wound healing, we tested the expression levels of the pro-inflammatory factors interleukin (IL)-1β and IL-6, the anti-inflammatory factor IL-10, and vascular endothelial growth factor (VEGF). The results showed that PAW promoted the release of pro-inflammatory factors and anti-inflammatory factors from the wounds in mice, which allowed the mice in the treatment group to transition out of the inflammatory period early and enter the next stage of wound healing. The expression level of VEGF in the wounds of mice in the PAW treatment group was higher, which indicates that the microvessels around the wound in the PAW treatment group proliferated faster, and thus the wound healed faster. PAW biosafety experiments showed that PAW did not significantly affect the appearance, morphology, or tissue structure of internal organs, or blood biochemical indicators in mice. In general, PAW prepared via portable devices is expected to become more widely used given its convenience, affordability, and lack of side effects in promoting wound healing.https://www.mdpi.com/2673-4125/1/3/22plasma-activated watermedical alcoholwound healinginflammatory factorvascular endothelial growth factor |
| spellingShingle | Shuai Wang Dehui Xu Miao Qi Bing Li Sansan Peng Qiaosong Li Hao Zhang Dingxin Liu Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses Biophysica plasma-activated water medical alcohol wound healing inflammatory factor vascular endothelial growth factor |
| title | Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses |
| title_full | Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses |
| title_fullStr | Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses |
| title_full_unstemmed | Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses |
| title_short | Plasma-Activated Water Promotes Wound Healing by Regulating Inflammatory Responses |
| title_sort | plasma activated water promotes wound healing by regulating inflammatory responses |
| topic | plasma-activated water medical alcohol wound healing inflammatory factor vascular endothelial growth factor |
| url | https://www.mdpi.com/2673-4125/1/3/22 |
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