Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise
The study aimed to assess the effect of a single bout of incremental exercise on irisin and BDNF plasma concentrations as related to erythrocyte purine nucleotides concentration at rest and after exercise. Master endurance master athletes (training experience 38 ± 6 years) and a group of untrained p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00923/full |
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| author | Ewa Rodziewicz Magdalena Król-Zielińska Jacek Zieliński Krzysztof Kusy Ewa Ziemann |
| author_facet | Ewa Rodziewicz Magdalena Król-Zielińska Jacek Zieliński Krzysztof Kusy Ewa Ziemann |
| author_sort | Ewa Rodziewicz |
| collection | DOAJ |
| description | The study aimed to assess the effect of a single bout of incremental exercise on irisin and BDNF plasma concentrations as related to erythrocyte purine nucleotides concentration at rest and after exercise. Master endurance master athletes (training experience 38 ± 6 years) and a group of untrained participants completed a single bout of progressive incremental exercise test until exhaustion. The dual-energy x-ray absorptiometry and blood collection were performed. Blood was taken twice at rest and 10 min after exercise. Concentrations of ATP, ADP, and AMP were assessed in the erythrocytes. Hypoxanthine and uric acid were determined in plasma using the high-performance liquid chromatography. Plasma concentrations of irisin and BDNF were assessed through the immunoenzymatic method. The ATP level, ATP/ADP ratio and AEC value were significantly higher in the athletic group. A significantly higher concentration of BDNF was it also noted in the trained group that correlated with the erythrocyte energy status at rest. The single session of exercise induced a significant increase in ATP erythrocyte levels in both groups. Both exerkines significantly correlated at rest with red blood cell adenine nucleotides and degradation products (BDNF positively and irisin negatively). The blood concentration of BDNF and irisin, in response to exercise, was not significantly different between groups. Obtained data revealed a higher erythrocyte energy status and lower purine degradation products concentration in master athletes. Also resting plasma exerkines differed substantially between groups. In conclusion, long-term training resulted in exercise adaptation reflected by a higher erythrocyte energy status, lower purine degradation products concentration and modified concentration of exerkines (higher BDNF and lower irisin blood concentrations). Therefore, we consider the training-induced adaptations in master athletes to be beneficial and significant. The moderate level of physical activity in the untrained group, even if sufficient in terms of general health, did not cause any discernible changes. |
| first_indexed | 2024-12-11T20:09:35Z |
| format | Article |
| id | doaj.art-c816c26df15d432496b9d7378d6ab2a7 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-11T20:09:35Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-c816c26df15d432496b9d7378d6ab2a72022-12-22T00:52:18ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00923556095Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of ExerciseEwa Rodziewicz0Magdalena Król-Zielińska1Jacek Zieliński2Krzysztof Kusy3Ewa Ziemann4Department of Physical Therapy and Biological Regeneration, Gdańsk University of Physical Education and Sport, Gdańsk, PolandDepartment of Physical Education and Lifelong Sports, Poznań University of Physical Education, Poznań, PolandDepartment of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, PolandDepartment of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, PolandDepartment of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, PolandThe study aimed to assess the effect of a single bout of incremental exercise on irisin and BDNF plasma concentrations as related to erythrocyte purine nucleotides concentration at rest and after exercise. Master endurance master athletes (training experience 38 ± 6 years) and a group of untrained participants completed a single bout of progressive incremental exercise test until exhaustion. The dual-energy x-ray absorptiometry and blood collection were performed. Blood was taken twice at rest and 10 min after exercise. Concentrations of ATP, ADP, and AMP were assessed in the erythrocytes. Hypoxanthine and uric acid were determined in plasma using the high-performance liquid chromatography. Plasma concentrations of irisin and BDNF were assessed through the immunoenzymatic method. The ATP level, ATP/ADP ratio and AEC value were significantly higher in the athletic group. A significantly higher concentration of BDNF was it also noted in the trained group that correlated with the erythrocyte energy status at rest. The single session of exercise induced a significant increase in ATP erythrocyte levels in both groups. Both exerkines significantly correlated at rest with red blood cell adenine nucleotides and degradation products (BDNF positively and irisin negatively). The blood concentration of BDNF and irisin, in response to exercise, was not significantly different between groups. Obtained data revealed a higher erythrocyte energy status and lower purine degradation products concentration in master athletes. Also resting plasma exerkines differed substantially between groups. In conclusion, long-term training resulted in exercise adaptation reflected by a higher erythrocyte energy status, lower purine degradation products concentration and modified concentration of exerkines (higher BDNF and lower irisin blood concentrations). Therefore, we consider the training-induced adaptations in master athletes to be beneficial and significant. The moderate level of physical activity in the untrained group, even if sufficient in terms of general health, did not cause any discernible changes.https://www.frontiersin.org/article/10.3389/fphys.2020.00923/fulladenylate metabolitespurine degradationslong-term endurance traininglactateexerkines |
| spellingShingle | Ewa Rodziewicz Magdalena Król-Zielińska Jacek Zieliński Krzysztof Kusy Ewa Ziemann Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise Frontiers in Physiology adenylate metabolites purine degradations long-term endurance training lactate exerkines |
| title | Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise |
| title_full | Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise |
| title_fullStr | Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise |
| title_full_unstemmed | Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise |
| title_short | Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise |
| title_sort | plasma concentration of irisin and brain derived neurotrophic factor and their association with the level of erythrocyte adenine nucleotides in response to long term endurance training at rest and after a single bout of exercise |
| topic | adenylate metabolites purine degradations long-term endurance training lactate exerkines |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00923/full |
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