The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco
The transcription factor SHE1 was induced by tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN) and SHE1 inhibited TMV accumulation when expressed constitutively. To better understand the role of SHE1 in virus infection, transgenic SNN tobacco plants generated to over-express SHE1 (...
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/1/59 |
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| author | Ju-Yeon Yoon Eseul Baek Mira Kim Peter Palukaitis |
| author_facet | Ju-Yeon Yoon Eseul Baek Mira Kim Peter Palukaitis |
| author_sort | Ju-Yeon Yoon |
| collection | DOAJ |
| description | The transcription factor SHE1 was induced by tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN) and SHE1 inhibited TMV accumulation when expressed constitutively. To better understand the role of SHE1 in virus infection, transgenic SNN tobacco plants generated to over-express SHE1 (OEx-SHE1) or silence expression of SHE1 (si-SHE1) were infected with TMV. OEx-SHE1 affected the local lesion resistance response to TMV, whereas si-SHE1 did not. However, si-SHE1 allowed a slow systemic infection to occur in SNN tobacco. An inhibitor of virus replication (IVR) was known to reduce the accumulation of TMV in SNN tobacco. Analysis of SHE1 and IVR mRNA levels in OEx-SHE1 plants showed constitutive expression of both mRNAs, whereas both mRNAs were less expressed in si-SHE1 plants, even after TMV infection, indicating that SHE1 and IVR were associated with a common signaling pathway. SHE1 and IVR interacted with each other in four different assay systems. The yeast two-hybrid assay also delimited sequences required for the interaction of these two proteins to the SHE1 central 58-79% region and the IVR C-terminal 50% of the protein sequences. This suggests that SHE is a transcription factor involved in the induction of IVR and that IVR binds to SHE1 to regulate its own synthesis. |
| first_indexed | 2024-03-09T11:03:34Z |
| format | Article |
| id | doaj.art-c81761527f1a44d99423f4bd55a4d69e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T11:03:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c81761527f1a44d99423f4bd55a4d69e2023-12-01T01:07:33ZengMDPI AGViruses1999-49152022-12-011515910.3390/v15010059The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene TobaccoJu-Yeon Yoon0Eseul Baek1Mira Kim2Peter Palukaitis3Department of Horticulture Sciences, Seoul Women's University, Seoul 01797, Republic of KoreaDepartment of Horticulture Sciences, Seoul Women's University, Seoul 01797, Republic of KoreaDepartment of Horticulture Sciences, Seoul Women's University, Seoul 01797, Republic of KoreaDepartment of Horticulture Sciences, Seoul Women's University, Seoul 01797, Republic of KoreaThe transcription factor SHE1 was induced by tobacco mosaic virus (TMV) infection in tobacco cv. Samsun NN (SNN) and SHE1 inhibited TMV accumulation when expressed constitutively. To better understand the role of SHE1 in virus infection, transgenic SNN tobacco plants generated to over-express SHE1 (OEx-SHE1) or silence expression of SHE1 (si-SHE1) were infected with TMV. OEx-SHE1 affected the local lesion resistance response to TMV, whereas si-SHE1 did not. However, si-SHE1 allowed a slow systemic infection to occur in SNN tobacco. An inhibitor of virus replication (IVR) was known to reduce the accumulation of TMV in SNN tobacco. Analysis of SHE1 and IVR mRNA levels in OEx-SHE1 plants showed constitutive expression of both mRNAs, whereas both mRNAs were less expressed in si-SHE1 plants, even after TMV infection, indicating that SHE1 and IVR were associated with a common signaling pathway. SHE1 and IVR interacted with each other in four different assay systems. The yeast two-hybrid assay also delimited sequences required for the interaction of these two proteins to the SHE1 central 58-79% region and the IVR C-terminal 50% of the protein sequences. This suggests that SHE is a transcription factor involved in the induction of IVR and that IVR binds to SHE1 to regulate its own synthesis.https://www.mdpi.com/1999-4915/15/1/59cucumber mosaic virus 1a proteininhibitor of virus replicationSHE1SHE1-IVR co-localizationSHE1-IVR interactionsignaling pathways |
| spellingShingle | Ju-Yeon Yoon Eseul Baek Mira Kim Peter Palukaitis The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco Viruses cucumber mosaic virus 1a protein inhibitor of virus replication SHE1 SHE1-IVR co-localization SHE1-IVR interaction signaling pathways |
| title | The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco |
| title_full | The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco |
| title_fullStr | The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco |
| title_full_unstemmed | The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco |
| title_short | The Virus-Induced Transcription Factor SHE1 Interacts with and Regulates Expression of the Inhibitor of Virus Replication (IVR) in <i>N</i> Gene Tobacco |
| title_sort | virus induced transcription factor she1 interacts with and regulates expression of the inhibitor of virus replication ivr in i n i gene tobacco |
| topic | cucumber mosaic virus 1a protein inhibitor of virus replication SHE1 SHE1-IVR co-localization SHE1-IVR interaction signaling pathways |
| url | https://www.mdpi.com/1999-4915/15/1/59 |
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