Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods
In this paper, the polysaccharides fingerprints of Polygonatum cyrtonema Hua. were constructed, combined with various chemometric methods, to evaluate the differences of P. cyrtonema polysaccharides from different origins. The 1-pheny-3-methyl-5-pyrazolone (PMP) pre-column derivatization-HPLC method...
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The editorial department of Science and Technology of Food Industry
2022-07-01
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Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100218 |
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author | Zhongyu ZHOU Zefei DU Tingting PU Liying YANG Renyi YAN Baozhong DUAN |
author_facet | Zhongyu ZHOU Zefei DU Tingting PU Liying YANG Renyi YAN Baozhong DUAN |
author_sort | Zhongyu ZHOU |
collection | DOAJ |
description | In this paper, the polysaccharides fingerprints of Polygonatum cyrtonema Hua. were constructed, combined with various chemometric methods, to evaluate the differences of P. cyrtonema polysaccharides from different origins. The 1-pheny-3-methyl-5-pyrazolone (PMP) pre-column derivatization-HPLC method was applied to analyze the monosaccharide composition and total polysaccharide content of P. cyrtonema. Furthermore, the fingerprints were further evaluated by chemometric methods, including similarity analysis (SA), cluster analysis (HCA), and principal component analysis (PCA). The results showed that the composition of monosaccharides was galacturonic acid, glucuronic acid, galactose, glucose, and xylose. The similarities were ranged from 0.781 to 0.945. In addition, the content of total polysaccharides was in the range of 7.18% to 16.27%. HCA and PCA all divided P. cyrtonema polysaccharides from different origins into two categories, the samples collected in Cili County, Hunan Province were a separate one. These results indicated that the polysaccharide fingerprints of P. cyrtonema were quite different. Establishing the standardized cultivation technology system of P. cyrtonema is necessary to ensure clinical efficacy and safety. |
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language | zho |
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publishDate | 2022-07-01 |
publisher | The editorial department of Science and Technology of Food Industry |
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spelling | doaj.art-c818e0c605084fdebaba6b2424fa24022022-12-22T02:52:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-01431328429010.13386/j.issn1002-0306.20211002182021100218-13Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric MethodsZhongyu ZHOU0Zefei DU1Tingting PU2Liying YANG3Renyi YAN4Baozhong DUAN5College of Pharmaceutical Science, Dali University, Dali 671000, ChinaCollege of Pharmaceutical Science, Dali University, Dali 671000, ChinaCollege of Pharmaceutical Science, Dali University, Dali 671000, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaHubei Hanjiang Health Industry Co., Ltd., Shiyan 442521, ChinaCollege of Pharmaceutical Science, Dali University, Dali 671000, ChinaIn this paper, the polysaccharides fingerprints of Polygonatum cyrtonema Hua. were constructed, combined with various chemometric methods, to evaluate the differences of P. cyrtonema polysaccharides from different origins. The 1-pheny-3-methyl-5-pyrazolone (PMP) pre-column derivatization-HPLC method was applied to analyze the monosaccharide composition and total polysaccharide content of P. cyrtonema. Furthermore, the fingerprints were further evaluated by chemometric methods, including similarity analysis (SA), cluster analysis (HCA), and principal component analysis (PCA). The results showed that the composition of monosaccharides was galacturonic acid, glucuronic acid, galactose, glucose, and xylose. The similarities were ranged from 0.781 to 0.945. In addition, the content of total polysaccharides was in the range of 7.18% to 16.27%. HCA and PCA all divided P. cyrtonema polysaccharides from different origins into two categories, the samples collected in Cili County, Hunan Province were a separate one. These results indicated that the polysaccharide fingerprints of P. cyrtonema were quite different. Establishing the standardized cultivation technology system of P. cyrtonema is necessary to ensure clinical efficacy and safety.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100218pre-column derivatizationpolygonatum cyrtonemapolysaccharidequality evaluationfingerprintchemometrics |
spellingShingle | Zhongyu ZHOU Zefei DU Tingting PU Liying YANG Renyi YAN Baozhong DUAN Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods Shipin gongye ke-ji pre-column derivatization polygonatum cyrtonema polysaccharide quality evaluation fingerprint chemometrics |
title | Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods |
title_full | Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods |
title_fullStr | Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods |
title_full_unstemmed | Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods |
title_short | Construction of PMP-HPLC Fingerprint of Partial Acid Hydrolysate of Polygonatum cyrtonema Polysaccharides Based on the Chemometric Methods |
title_sort | construction of pmp hplc fingerprint of partial acid hydrolysate of polygonatum cyrtonema polysaccharides based on the chemometric methods |
topic | pre-column derivatization polygonatum cyrtonema polysaccharide quality evaluation fingerprint chemometrics |
url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100218 |
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