<em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects
Bioactive glasses (BGs) are being increasingly considered for numerous biomedical applications. The loading of natural compounds onto BGs to increase the BG biological activity is receiving increasing attention. However, achieving efficient loading of phytotherapeutic compounds onto the surface of b...
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MDPI AG
2022-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/1/126 |
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| author | Kanwal Ilyas Lamia Singer Muhammad Asim Akhtar Christoph P. Bourauel Aldo R. Boccaccini |
| author_facet | Kanwal Ilyas Lamia Singer Muhammad Asim Akhtar Christoph P. Bourauel Aldo R. Boccaccini |
| author_sort | Kanwal Ilyas |
| collection | DOAJ |
| description | Bioactive glasses (BGs) are being increasingly considered for numerous biomedical applications. The loading of natural compounds onto BGs to increase the BG biological activity is receiving increasing attention. However, achieving efficient loading of phytotherapeutic compounds onto the surface of bioactive glass is challenging. The present work aimed to prepare novel amino-functionalized mesoporous bioactive glass nanoparticles (MBGNs) loaded with the phytotherapeutic agent <i>Boswellia sacra</i> extract. The prepared amino-functionalized MBGNs showed suitable loading capacity and releasing time. MBGNs (nominal composition: 58 wt% SiO<sub>2</sub>, 37 wt% CaO, 5 wt% P<sub>2</sub>O<sub>5</sub>) were prepared by sol-gel-modified co-precipitation method and were successfully surface-modified by using 3-aminopropyltriethoxysilane (APTES). In order to evaluate MBGNs loaded with <i>Boswellia sacra</i>, morphological analysis, biological studies, physico-chemical and release studies were performed. The successful functionalization and loading of the natural compound were confirmed with FTIR, zeta-potential measurements and UV-Vis spectroscopy, respectively. Structural and morphological evaluation of MBGNs was done by XRD, SEM and BET analyses, whereas the chemical analysis of the plant extract was done using GC/MS technique. The functionalized MBGNs showed high loading capacity as compared to non-functionalized MBGNs. The release studies revealed that <i>Boswellia sacra</i> molecules were released via controlled diffusion and led to antibacterial effects against <i>S. aureus</i> (Gram-positive) bacteria. Results of cell culture studies using human osteoblastic-like cells (MG-63) indicated better cell viability of the <i>Boswellia sacra</i>-loaded MBGNs as compared to the unloaded MBGNs. Therefore, the strategy of combining the properties of MBGNs with the therapeutic effects of <i>Boswellia sacra</i> represents a novel, convenient step towards the development of phytotherapeutic-loaded antibacterial, inorganic materials to improve tissue healing and regeneration. |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T00:43:31Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-c8196d8e8f3c46949710d6d64603b4c92023-11-23T15:04:21ZengMDPI AGPharmaceutics1999-49232022-01-0114112610.3390/pharmaceutics14010126<em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological EffectsKanwal Ilyas0Lamia Singer1Muhammad Asim Akhtar2Christoph P. Bourauel3Aldo R. Boccaccini4Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, GermanyOral Technology, Dental School, University Hospital Bonn, Welschnonnenstr. 17, 53111 Bonn, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, GermanyOral Technology, Dental School, University Hospital Bonn, Welschnonnenstr. 17, 53111 Bonn, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, GermanyBioactive glasses (BGs) are being increasingly considered for numerous biomedical applications. The loading of natural compounds onto BGs to increase the BG biological activity is receiving increasing attention. However, achieving efficient loading of phytotherapeutic compounds onto the surface of bioactive glass is challenging. The present work aimed to prepare novel amino-functionalized mesoporous bioactive glass nanoparticles (MBGNs) loaded with the phytotherapeutic agent <i>Boswellia sacra</i> extract. The prepared amino-functionalized MBGNs showed suitable loading capacity and releasing time. MBGNs (nominal composition: 58 wt% SiO<sub>2</sub>, 37 wt% CaO, 5 wt% P<sub>2</sub>O<sub>5</sub>) were prepared by sol-gel-modified co-precipitation method and were successfully surface-modified by using 3-aminopropyltriethoxysilane (APTES). In order to evaluate MBGNs loaded with <i>Boswellia sacra</i>, morphological analysis, biological studies, physico-chemical and release studies were performed. The successful functionalization and loading of the natural compound were confirmed with FTIR, zeta-potential measurements and UV-Vis spectroscopy, respectively. Structural and morphological evaluation of MBGNs was done by XRD, SEM and BET analyses, whereas the chemical analysis of the plant extract was done using GC/MS technique. The functionalized MBGNs showed high loading capacity as compared to non-functionalized MBGNs. The release studies revealed that <i>Boswellia sacra</i> molecules were released via controlled diffusion and led to antibacterial effects against <i>S. aureus</i> (Gram-positive) bacteria. Results of cell culture studies using human osteoblastic-like cells (MG-63) indicated better cell viability of the <i>Boswellia sacra</i>-loaded MBGNs as compared to the unloaded MBGNs. Therefore, the strategy of combining the properties of MBGNs with the therapeutic effects of <i>Boswellia sacra</i> represents a novel, convenient step towards the development of phytotherapeutic-loaded antibacterial, inorganic materials to improve tissue healing and regeneration.https://www.mdpi.com/1999-4923/14/1/126bioactive glassfunctionalization<i>Boswellia sacra</i>phytotherapeuticsantibacterial |
| spellingShingle | Kanwal Ilyas Lamia Singer Muhammad Asim Akhtar Christoph P. Bourauel Aldo R. Boccaccini <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects Pharmaceutics bioactive glass functionalization <i>Boswellia sacra</i> phytotherapeutics antibacterial |
| title | <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects |
| title_full | <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects |
| title_fullStr | <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects |
| title_full_unstemmed | <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects |
| title_short | <em>Boswellia sacra</em> Extract-Loaded Mesoporous Bioactive Glass Nano Particles: Synthesis and Biological Effects |
| title_sort | em boswellia sacra em extract loaded mesoporous bioactive glass nano particles synthesis and biological effects |
| topic | bioactive glass functionalization <i>Boswellia sacra</i> phytotherapeutics antibacterial |
| url | https://www.mdpi.com/1999-4923/14/1/126 |
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