Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study
Background and purposeNecrotizing enterocolitis (NEC) is a critical gastrointestinal disease. We aim to explore the value of fecal human β-defensin 2 (HBD-2), Claudin-3, high-mobility group box-1 protein (HMGB-1), and resistin-like molecule β (Relmβ) as well as some laboratory metrics to predict the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Pediatrics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2022.1062798/full |
| _version_ | 1811293605921292288 |
|---|---|
| author | Xiao-Chen Liu Lu-Quan Li Ke-Ran Ling Lu Guo Xiao-Yu Hu Chun Li |
| author_facet | Xiao-Chen Liu Lu-Quan Li Ke-Ran Ling Lu Guo Xiao-Yu Hu Chun Li |
| author_sort | Xiao-Chen Liu |
| collection | DOAJ |
| description | Background and purposeNecrotizing enterocolitis (NEC) is a critical gastrointestinal disease. We aim to explore the value of fecal human β-defensin 2 (HBD-2), Claudin-3, high-mobility group box-1 protein (HMGB-1), and resistin-like molecule β (Relmβ) as well as some laboratory metrics to predict the deterioration of NEC.MethodsInfants diagnosed with NEC at Stage II were enrolled in our study. Those who progressed to Stage III were included in the Stage III group and the rest were included in the Stage II group. Clinical data and laboratory metrics of the infants were collected. Fecal samples of HBD2, HMGB-1, Claudin-3, and Relmβ collected during their enrollment were determined by using enzyme-linked immunosorbent assay (ELISA) kits. Student's t-test, the Mann–Whitney U test, the chi-square test, receiver operating characteristic (ROC), and logistic regression analysis were performed.ResultsSixty infants diagnosed with NEC at Stage II were enrolled in our study, with 27 in the Stage III group (n = 27) and 33 in the Stage II group (n = 33). Although many of these NEC cases were late preterm and term infants, the infants in the Stage III group had a lower gestational age (P < 0.05). The incidence of gestational diabetes mellitus, peritonitis, intestinal adhesion, and sepsis was higher and more infants in the Stage III group underwent surgeries (P < 0.05). The levels of HBD-2 and Claudin-3 were higher and neutrophil count was lower in the Stage III group than in the Stage II Group, and the area under the curve (AUC) was 0.754, 0,755, and 0.666, respectively (P < 0.05). HBD-2 ≥ 1649.02 ng/g and Claudin-3 ≥ 2488.71 pg/g were included in the multivariate stepwise logistic regression analysis (P < 0.05), and the AUC of the model was 0.805 (95% CI: 0.688–0.922).ConclusionFecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of NEC from Stage II to Stage III. |
| first_indexed | 2024-04-13T05:03:57Z |
| format | Article |
| id | doaj.art-c81e0bd4ec8a4354a4e74861396e4b84 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-04-13T05:03:57Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-c81e0bd4ec8a4354a4e74861396e4b842022-12-22T03:01:14ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602022-12-011010.3389/fped.2022.10627981062798Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective studyXiao-Chen LiuLu-Quan LiKe-Ran LingLu GuoXiao-Yu HuChun LiBackground and purposeNecrotizing enterocolitis (NEC) is a critical gastrointestinal disease. We aim to explore the value of fecal human β-defensin 2 (HBD-2), Claudin-3, high-mobility group box-1 protein (HMGB-1), and resistin-like molecule β (Relmβ) as well as some laboratory metrics to predict the deterioration of NEC.MethodsInfants diagnosed with NEC at Stage II were enrolled in our study. Those who progressed to Stage III were included in the Stage III group and the rest were included in the Stage II group. Clinical data and laboratory metrics of the infants were collected. Fecal samples of HBD2, HMGB-1, Claudin-3, and Relmβ collected during their enrollment were determined by using enzyme-linked immunosorbent assay (ELISA) kits. Student's t-test, the Mann–Whitney U test, the chi-square test, receiver operating characteristic (ROC), and logistic regression analysis were performed.ResultsSixty infants diagnosed with NEC at Stage II were enrolled in our study, with 27 in the Stage III group (n = 27) and 33 in the Stage II group (n = 33). Although many of these NEC cases were late preterm and term infants, the infants in the Stage III group had a lower gestational age (P < 0.05). The incidence of gestational diabetes mellitus, peritonitis, intestinal adhesion, and sepsis was higher and more infants in the Stage III group underwent surgeries (P < 0.05). The levels of HBD-2 and Claudin-3 were higher and neutrophil count was lower in the Stage III group than in the Stage II Group, and the area under the curve (AUC) was 0.754, 0,755, and 0.666, respectively (P < 0.05). HBD-2 ≥ 1649.02 ng/g and Claudin-3 ≥ 2488.71 pg/g were included in the multivariate stepwise logistic regression analysis (P < 0.05), and the AUC of the model was 0.805 (95% CI: 0.688–0.922).ConclusionFecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of NEC from Stage II to Stage III.https://www.frontiersin.org/articles/10.3389/fped.2022.1062798/fullhuman β-defensin 2claudin-3biomarkerdeteriorationnecrotizing enterocolitis |
| spellingShingle | Xiao-Chen Liu Lu-Quan Li Ke-Ran Ling Lu Guo Xiao-Yu Hu Chun Li Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study Frontiers in Pediatrics human β-defensin 2 claudin-3 biomarker deterioration necrotizing enterocolitis |
| title | Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study |
| title_full | Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study |
| title_fullStr | Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study |
| title_full_unstemmed | Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study |
| title_short | Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study |
| title_sort | fecal hbd 2 and claudin 3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis a prospective study |
| topic | human β-defensin 2 claudin-3 biomarker deterioration necrotizing enterocolitis |
| url | https://www.frontiersin.org/articles/10.3389/fped.2022.1062798/full |
| work_keys_str_mv | AT xiaochenliu fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy AT luquanli fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy AT keranling fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy AT luguo fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy AT xiaoyuhu fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy AT chunli fecalhbd2andclaudin3maybepotentialbiomarkerstopredictthedeteriorationofnecrotizingenterocolitisaprospectivestudy |