Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex
Fungal-infections are mostly due to fungi in an adhering, biofilm-mode of growth and not due to planktonically growing, suspended-fungi. 1, 8-cineole is a natural product, which has been shown to possess antifungal effect. However, the anti-biofilm effect and mechanism of 1,8-cineole against Fusariu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1010593/full |
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| author | Yu Zhang Yiming Wang Xinghong Zhao Lu Liu Rui Xing Xu Song Yuanfeng Zou Lixia Li Hongping Wan Renyong Jia Lizi Yin Xiaoxia Liang Changliang He Qin Wei Zhongqiong Yin |
| author_facet | Yu Zhang Yiming Wang Xinghong Zhao Lu Liu Rui Xing Xu Song Yuanfeng Zou Lixia Li Hongping Wan Renyong Jia Lizi Yin Xiaoxia Liang Changliang He Qin Wei Zhongqiong Yin |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Fungal-infections are mostly due to fungi in an adhering, biofilm-mode of growth and not due to planktonically growing, suspended-fungi. 1, 8-cineole is a natural product, which has been shown to possess antifungal effect. However, the anti-biofilm effect and mechanism of 1,8-cineole against Fusarium solani species complex has not reported previously. In this study, we found that 1,8-cineole has a good antifungal activity against F. solani with an MIC value of 46.1 μg/ml. Notably, 1,8-cineole showed good anti-biofilm formation activity against F. solani via inhibiting cell adhesion, hypha formation and decreasing the secretion of extracellular matrix at the concentration of ≥5.76 μg/ml. In addition, transcriptome sequencing analysis results showed that F. solani species complex genes related to ECM, protein synthesis and energy metabolism were down-expressed in the biofilms formation process treated with 1,8-cineole. In conclusion, these results show that 1,8-cineole has good anti-biofilm formation activity against F. solani species complex, and it exerts its anti-biofilm formation activity by downregulating of ergosterol biosynthetic genes, inhibiting adhesion, hindering the synthesis of ECM and interfering mitochondrial activity. This study suggests that 1,8-cineole is a promising anti-biofilm agent against F. solani species complex. |
| first_indexed | 2024-04-11T09:23:59Z |
| format | Article |
| id | doaj.art-c820ea9110bb46f6a905d69566837fa9 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T09:23:59Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-c820ea9110bb46f6a905d69566837fa92022-12-22T04:32:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-10-011310.3389/fphar.2022.10105931010593Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complexYu Zhang0Yiming Wang1Xinghong Zhao2Lu Liu3Rui Xing4Xu Song5Yuanfeng Zou6Lixia Li7Hongping Wan8Renyong Jia9Lizi Yin10Xiaoxia Liang11Changliang He12Qin Wei13Zhongqiong Yin14Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaYibin university Sichuan Oil Cinnamon Engineering Technology Research Center, Yibin University, Yibin, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaFungal-infections are mostly due to fungi in an adhering, biofilm-mode of growth and not due to planktonically growing, suspended-fungi. 1, 8-cineole is a natural product, which has been shown to possess antifungal effect. However, the anti-biofilm effect and mechanism of 1,8-cineole against Fusarium solani species complex has not reported previously. In this study, we found that 1,8-cineole has a good antifungal activity against F. solani with an MIC value of 46.1 μg/ml. Notably, 1,8-cineole showed good anti-biofilm formation activity against F. solani via inhibiting cell adhesion, hypha formation and decreasing the secretion of extracellular matrix at the concentration of ≥5.76 μg/ml. In addition, transcriptome sequencing analysis results showed that F. solani species complex genes related to ECM, protein synthesis and energy metabolism were down-expressed in the biofilms formation process treated with 1,8-cineole. In conclusion, these results show that 1,8-cineole has good anti-biofilm formation activity against F. solani species complex, and it exerts its anti-biofilm formation activity by downregulating of ergosterol biosynthetic genes, inhibiting adhesion, hindering the synthesis of ECM and interfering mitochondrial activity. This study suggests that 1,8-cineole is a promising anti-biofilm agent against F. solani species complex.https://www.frontiersin.org/articles/10.3389/fphar.2022.1010593/fullFusarium solani species complexbiofilm18-cineoleadhesionmitochondrial |
| spellingShingle | Yu Zhang Yiming Wang Xinghong Zhao Lu Liu Rui Xing Xu Song Yuanfeng Zou Lixia Li Hongping Wan Renyong Jia Lizi Yin Xiaoxia Liang Changliang He Qin Wei Zhongqiong Yin Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex Frontiers in Pharmacology Fusarium solani species complex biofilm 1 8-cineole adhesion mitochondrial |
| title | Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex |
| title_full | Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex |
| title_fullStr | Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex |
| title_full_unstemmed | Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex |
| title_short | Study on the anti-biofilm mechanism of 1,8-cineole against Fusarium solani species complex |
| title_sort | study on the anti biofilm mechanism of 1 8 cineole against fusarium solani species complex |
| topic | Fusarium solani species complex biofilm 1 8-cineole adhesion mitochondrial |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1010593/full |
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