Quantifying colocalization: thresholding, void voxels and the H(coef).

A critical step in the analysis of images is identifying the area of interest e.g. nuclei. When the nuclei are brighter than the remainder of the image an intensity can be chosen to identify the nuclei. Intensity thresholding is complicated by variations in the intensity of individual nuclei and the...

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Bibliographic Details
Main Authors: Jeremy Adler, Ingela Parmryd
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4222960?pdf=render