Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply
Abstract Understanding the mechanisms underlying variations in carbon isotope discrimination (Δ) in C4 plants is critical for predicting the C3/C4 ratio in C3/C4 mixed grassland. The value of Δ is determined by bundle sheath leakiness (Ф) and the ratio of intercellular to ambient CO2 concentration (...
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| Format: | Article |
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Nature Portfolio
2017-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-00498-w |
| _version_ | 1818422084858019840 |
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| author | Hao Yang Qiang Yu Wen-ping Sheng Sheng-gong Li Jing Tian |
| author_facet | Hao Yang Qiang Yu Wen-ping Sheng Sheng-gong Li Jing Tian |
| author_sort | Hao Yang |
| collection | DOAJ |
| description | Abstract Understanding the mechanisms underlying variations in carbon isotope discrimination (Δ) in C4 plants is critical for predicting the C3/C4 ratio in C3/C4 mixed grassland. The value of Δ is determined by bundle sheath leakiness (Ф) and the ratio of intercellular to ambient CO2 concentration (C i /C a ). Leaf nitrogen concentration (N leaf ) is considered a driver of Δ in C4 plants. However, little is known about how N leaf affects Ф and C i /C a , and subsequently Δ. Here leaf carbon isotope composition, N leaf , Ф, and leaf gas exchange were measured in Cleistogenes squarrosa, a dominant C4 species in the Inner Mongolia grassland. Δ remained relatively stable under variable N and water supply. Higher N supply and lower water supply increased N leaf , stimulated photosynthesis and further decreased C i /C a . High N supply increased Ф, which responded weakly to water supply. N leaf exerted similar effects on C i /C a and on Ф in the field and pot experiments. Pooling all the data, N leaf explained 73% of the variation in C i /C a . Overall, both Ф and C i /C a determined Δ; however, the contribution of Ф was stronger. N leaf influenced Δ primarily though C i /C a , rather than Ф. Ф should be considered in estimating Δ of C4 endmember. |
| first_indexed | 2024-12-14T13:20:38Z |
| format | Article |
| id | doaj.art-c82fc060146646478d7b2326ee675af2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T13:20:38Z |
| publishDate | 2017-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c82fc060146646478d7b2326ee675af22022-12-21T22:59:57ZengNature PortfolioScientific Reports2045-23222017-03-01711810.1038/s41598-017-00498-wDetermination of leaf carbon isotope discrimination in C4 plants under variable N and water supplyHao Yang0Qiang Yu1Wen-ping Sheng2Sheng-gong Li3Jing Tian4Key Laboratory of Ecosystem Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of SciencesNational Hulunber Grassland Ecosystem Observation and Research Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesKey Laboratory of Ecosystem Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of SciencesKey Laboratory of Ecosystem Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of SciencesKey Laboratory of Ecosystem Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of SciencesAbstract Understanding the mechanisms underlying variations in carbon isotope discrimination (Δ) in C4 plants is critical for predicting the C3/C4 ratio in C3/C4 mixed grassland. The value of Δ is determined by bundle sheath leakiness (Ф) and the ratio of intercellular to ambient CO2 concentration (C i /C a ). Leaf nitrogen concentration (N leaf ) is considered a driver of Δ in C4 plants. However, little is known about how N leaf affects Ф and C i /C a , and subsequently Δ. Here leaf carbon isotope composition, N leaf , Ф, and leaf gas exchange were measured in Cleistogenes squarrosa, a dominant C4 species in the Inner Mongolia grassland. Δ remained relatively stable under variable N and water supply. Higher N supply and lower water supply increased N leaf , stimulated photosynthesis and further decreased C i /C a . High N supply increased Ф, which responded weakly to water supply. N leaf exerted similar effects on C i /C a and on Ф in the field and pot experiments. Pooling all the data, N leaf explained 73% of the variation in C i /C a . Overall, both Ф and C i /C a determined Δ; however, the contribution of Ф was stronger. N leaf influenced Δ primarily though C i /C a , rather than Ф. Ф should be considered in estimating Δ of C4 endmember.https://doi.org/10.1038/s41598-017-00498-w |
| spellingShingle | Hao Yang Qiang Yu Wen-ping Sheng Sheng-gong Li Jing Tian Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply Scientific Reports |
| title | Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply |
| title_full | Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply |
| title_fullStr | Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply |
| title_full_unstemmed | Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply |
| title_short | Determination of leaf carbon isotope discrimination in C4 plants under variable N and water supply |
| title_sort | determination of leaf carbon isotope discrimination in c4 plants under variable n and water supply |
| url | https://doi.org/10.1038/s41598-017-00498-w |
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