Renewable and tuneable bio-LPG blends derived from amino acids
Abstract Background Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have used a terminal fatty acid photodecarboxylase, enabling light-driven pro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01766-0 |
| _version_ | 1818232867302408192 |
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| author | Mohamed Amer Robin Hoeven Paul Kelly Matthew Faulkner Michael H. Smith Helen S. Toogood Nigel S. Scrutton |
| author_facet | Mohamed Amer Robin Hoeven Paul Kelly Matthew Faulkner Michael H. Smith Helen S. Toogood Nigel S. Scrutton |
| author_sort | Mohamed Amer |
| collection | DOAJ |
| description | Abstract Background Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have used a terminal fatty acid photodecarboxylase, enabling light-driven propane production from externally fed waste butyric acid. Use of Halomonas (a robust extremophile microbial chassis) with these pathways has enabled bio-LPG production under non-sterile conditions and using waste biomass as the carbon source. Here, we describe new engineering approaches to produce next-generation pathways that use amino acids as fuel precursors for bio-LPG production (propane, butane and isobutane blends). Results Multiple pathways from the amino acids valine, leucine and isoleucine were designed in E. coli for the production of propane, isobutane and butane, respectively. A branched-chain keto acid decarboxylase-dependent pathway utilising fatty acid photodecarboxylase was the most effective route, generating higher alkane gas titres over alternative routes requiring coenzyme A and/or aldehyde deformylating oxygenase. Isobutane was the major gas produced in standard (mixed amino acid) medium, however valine supplementation led to primarily propane production. Transitioning pathways into Halomonas strain TQ10 enabled fermentative production of mixed alkane gases under non-sterile conditions on simple carbon sources. Chromosomal integration of inducible (~ 180 mg/g cells/day) and constitutive (~ 30 mg/g cells/day) pathways into Halomonas generated production strains shown to be stable for up to 7 days. Conclusions This study highlights new microbial pathways for the production of clean-burning bio-LPG fuels from amino acids. The use of stable Halomonas production strains could lead to gas production in the field under non-sterile conditions following process optimisation. |
| first_indexed | 2024-12-12T11:13:06Z |
| format | Article |
| id | doaj.art-c830332dbd02431ab0490eb09c0c4163 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T11:13:06Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-c830332dbd02431ab0490eb09c0c41632022-12-22T00:26:15ZengBMCBiotechnology for Biofuels1754-68342020-07-0113111510.1186/s13068-020-01766-0Renewable and tuneable bio-LPG blends derived from amino acidsMohamed Amer0Robin Hoeven1Paul Kelly2Matthew Faulkner3Michael H. Smith4Helen S. Toogood5Nigel S. Scrutton6EPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterEPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterEPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterEPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterC3 Biotechnologies LtdEPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterEPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC, Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology and School of Chemistry, The University of ManchesterAbstract Background Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have used a terminal fatty acid photodecarboxylase, enabling light-driven propane production from externally fed waste butyric acid. Use of Halomonas (a robust extremophile microbial chassis) with these pathways has enabled bio-LPG production under non-sterile conditions and using waste biomass as the carbon source. Here, we describe new engineering approaches to produce next-generation pathways that use amino acids as fuel precursors for bio-LPG production (propane, butane and isobutane blends). Results Multiple pathways from the amino acids valine, leucine and isoleucine were designed in E. coli for the production of propane, isobutane and butane, respectively. A branched-chain keto acid decarboxylase-dependent pathway utilising fatty acid photodecarboxylase was the most effective route, generating higher alkane gas titres over alternative routes requiring coenzyme A and/or aldehyde deformylating oxygenase. Isobutane was the major gas produced in standard (mixed amino acid) medium, however valine supplementation led to primarily propane production. Transitioning pathways into Halomonas strain TQ10 enabled fermentative production of mixed alkane gases under non-sterile conditions on simple carbon sources. Chromosomal integration of inducible (~ 180 mg/g cells/day) and constitutive (~ 30 mg/g cells/day) pathways into Halomonas generated production strains shown to be stable for up to 7 days. Conclusions This study highlights new microbial pathways for the production of clean-burning bio-LPG fuels from amino acids. The use of stable Halomonas production strains could lead to gas production in the field under non-sterile conditions following process optimisation.http://link.springer.com/article/10.1186/s13068-020-01766-0BiofuelsPropaneIsobutaneButaneMicrobial pathway engineeringEscherichia coli |
| spellingShingle | Mohamed Amer Robin Hoeven Paul Kelly Matthew Faulkner Michael H. Smith Helen S. Toogood Nigel S. Scrutton Renewable and tuneable bio-LPG blends derived from amino acids Biotechnology for Biofuels Biofuels Propane Isobutane Butane Microbial pathway engineering Escherichia coli |
| title | Renewable and tuneable bio-LPG blends derived from amino acids |
| title_full | Renewable and tuneable bio-LPG blends derived from amino acids |
| title_fullStr | Renewable and tuneable bio-LPG blends derived from amino acids |
| title_full_unstemmed | Renewable and tuneable bio-LPG blends derived from amino acids |
| title_short | Renewable and tuneable bio-LPG blends derived from amino acids |
| title_sort | renewable and tuneable bio lpg blends derived from amino acids |
| topic | Biofuels Propane Isobutane Butane Microbial pathway engineering Escherichia coli |
| url | http://link.springer.com/article/10.1186/s13068-020-01766-0 |
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