Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst
The beneficial photocatalytic properties of UV light activated TiO<sub>2</sub> powder are well-known and have been demonstrated with various pollutants and pathogens. However, traditionally observed photocatalytic activity of visible light activated pristine TiO<sub>2</sub> i...
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MDPI AG
2021-11-01
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| author | Sandra Sakalauskaite Deimante Vasiliauske Emilija Demikyte Rimantas Daugelavicius Martynas Lelis |
| author_facet | Sandra Sakalauskaite Deimante Vasiliauske Emilija Demikyte Rimantas Daugelavicius Martynas Lelis |
| author_sort | Sandra Sakalauskaite |
| collection | DOAJ |
| description | The beneficial photocatalytic properties of UV light activated TiO<sub>2</sub> powder are well-known and have been demonstrated with various pollutants and pathogens. However, traditionally observed photocatalytic activity of visible light activated pristine TiO<sub>2</sub> is insignificant but there are a few studies which have reported that under some specific conditions commercially available TiO<sub>2</sub> powder could at least partially disinfect microorganisms even under visible light. To better understand this phenomenon, in the current study we focused on bacteria response to the treatment by visible light and P25 TiO<sub>2</sub> powder. More specifically, we analyzed the relationship between the bacteria viability, outer membrane permeability, metabolism, and its capacity to generate intracellular reactive oxygen species. During the study we assayed the viability of treated bacteria by the spread plate technique and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. Changes in bacterial outer membrane permeability were determined by measuring the fluorescence of N-phenyl-1-naphthylamine (NPN). To detect intracellular reactive oxygen species formation, the fluorescence of dichlorodihydrofluorescein diacetate (DCFH-DA) was assayed. Results of our study indicated that TiO<sub>2</sub> and wide spectrum visible light irradiation damaged the integrity of the outer membrane and caused oxidative stress in the metabolizing bacteria. When favorable conditions were created, these effects added up and unexpectedly high bacterial inactivation was achieved. |
| first_indexed | 2024-03-10T04:31:12Z |
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| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T04:31:12Z |
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| spelling | doaj.art-c83316127e2246b7a1943b9337287c2b2023-11-23T04:08:51ZengMDPI AGCatalysts2073-43442021-11-011112143210.3390/catal11121432Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> PhotocatalystSandra Sakalauskaite0Deimante Vasiliauske1Emilija Demikyte2Rimantas Daugelavicius3Martynas Lelis4Faculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos St., 44404 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos St., 44404 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos St., 44404 Kaunas, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos St., 44404 Kaunas, LithuaniaCentre for Hydrogen Energy Technologies, Lithuanian Energy Institute, 3 Breslaujos St., 44403 Kaunas, LithuaniaThe beneficial photocatalytic properties of UV light activated TiO<sub>2</sub> powder are well-known and have been demonstrated with various pollutants and pathogens. However, traditionally observed photocatalytic activity of visible light activated pristine TiO<sub>2</sub> is insignificant but there are a few studies which have reported that under some specific conditions commercially available TiO<sub>2</sub> powder could at least partially disinfect microorganisms even under visible light. To better understand this phenomenon, in the current study we focused on bacteria response to the treatment by visible light and P25 TiO<sub>2</sub> powder. More specifically, we analyzed the relationship between the bacteria viability, outer membrane permeability, metabolism, and its capacity to generate intracellular reactive oxygen species. During the study we assayed the viability of treated bacteria by the spread plate technique and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. Changes in bacterial outer membrane permeability were determined by measuring the fluorescence of N-phenyl-1-naphthylamine (NPN). To detect intracellular reactive oxygen species formation, the fluorescence of dichlorodihydrofluorescein diacetate (DCFH-DA) was assayed. Results of our study indicated that TiO<sub>2</sub> and wide spectrum visible light irradiation damaged the integrity of the outer membrane and caused oxidative stress in the metabolizing bacteria. When favorable conditions were created, these effects added up and unexpectedly high bacterial inactivation was achieved.https://www.mdpi.com/2073-4344/11/12/1432<i>S.</i> Typhimuriumbacteriophagesmetabolic activitydisinfectionreactive oxygen speciesoxidative stress |
| spellingShingle | Sandra Sakalauskaite Deimante Vasiliauske Emilija Demikyte Rimantas Daugelavicius Martynas Lelis Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst Catalysts <i>S.</i> Typhimurium bacteriophages metabolic activity disinfection reactive oxygen species oxidative stress |
| title | Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst |
| title_full | Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst |
| title_fullStr | Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst |
| title_full_unstemmed | Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst |
| title_short | Influence of the Metabolic Activity of Microorganisms on Disinfection Efficiency of the Visible Light and P25 TiO<sub>2</sub> Photocatalyst |
| title_sort | influence of the metabolic activity of microorganisms on disinfection efficiency of the visible light and p25 tio sub 2 sub photocatalyst |
| topic | <i>S.</i> Typhimurium bacteriophages metabolic activity disinfection reactive oxygen species oxidative stress |
| url | https://www.mdpi.com/2073-4344/11/12/1432 |
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