Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein
The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/785 |
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| author | Ting-Chou Chang Aileen Y. Sun Yu-Chung Huang Chih-Hui Wang Shau-Chun Wang Lai-Kwan Chau |
| author_facet | Ting-Chou Chang Aileen Y. Sun Yu-Chung Huang Chih-Hui Wang Shau-Chun Wang Lai-Kwan Chau |
| author_sort | Ting-Chou Chang |
| collection | DOAJ |
| description | The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus and it serves as a useful diagnostic marker for detection. Herein, we report a fiber optic particle plasmon resonance (FOPPR) biosensor which employed a single-stranded DNA (ssDNA) aptamer as the recognition element to detect the SARS-CoV-2 N-protein in 15 min with a limit of detection (LOD) of 2.8 nM, meeting the acceptable LOD of 10<sup>6</sup> copies/mL set by the WHO target product profile. The sensor chip is a microfluidic chip based on the balance between the gravitational potential and the capillary force to control fluid loading, thus enabling the power-free auto-flowing function. It also has a risk-free self-contained design to avoid the risk of the virus leaking into the environment. These findings demonstrate the potential for designing a low-cost and robust POCT device towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants. |
| first_indexed | 2024-03-09T20:36:18Z |
| format | Article |
| id | doaj.art-c837c5591095464d84daf7141f012f93 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:36:18Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-c837c5591095464d84daf7141f012f932023-11-23T23:10:37ZengMDPI AGBiosensors2079-63742022-09-01121078510.3390/bios12100785Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid ProteinTing-Chou Chang0Aileen Y. Sun1Yu-Chung Huang2Chih-Hui Wang3Shau-Chun Wang4Lai-Kwan Chau5Center for Nano Bio-Detection, National Chung Cheng University, Chiayi 621301, TaiwanInstant NanoBiosensors, Co., Ltd., Taipei 115010, TaiwanInstant NanoBiosensors, Co., Ltd., Taipei 115010, TaiwanCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi 621301, TaiwanCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi 621301, TaiwanCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi 621301, TaiwanThe global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus and it serves as a useful diagnostic marker for detection. Herein, we report a fiber optic particle plasmon resonance (FOPPR) biosensor which employed a single-stranded DNA (ssDNA) aptamer as the recognition element to detect the SARS-CoV-2 N-protein in 15 min with a limit of detection (LOD) of 2.8 nM, meeting the acceptable LOD of 10<sup>6</sup> copies/mL set by the WHO target product profile. The sensor chip is a microfluidic chip based on the balance between the gravitational potential and the capillary force to control fluid loading, thus enabling the power-free auto-flowing function. It also has a risk-free self-contained design to avoid the risk of the virus leaking into the environment. These findings demonstrate the potential for designing a low-cost and robust POCT device towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants.https://www.mdpi.com/2079-6374/12/10/785fiber optic biosensorgold nanoparticlelocalized surface plasmon resonanceaptamermicrofluidic chipbinding kinetics |
| spellingShingle | Ting-Chou Chang Aileen Y. Sun Yu-Chung Huang Chih-Hui Wang Shau-Chun Wang Lai-Kwan Chau Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein Biosensors fiber optic biosensor gold nanoparticle localized surface plasmon resonance aptamer microfluidic chip binding kinetics |
| title | Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein |
| title_full | Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein |
| title_fullStr | Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein |
| title_full_unstemmed | Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein |
| title_short | Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein |
| title_sort | integration of power free and self contained microfluidic chip with fiber optic particle plasmon resonance aptasensor for rapid detection of sars cov 2 nucleocapsid protein |
| topic | fiber optic biosensor gold nanoparticle localized surface plasmon resonance aptamer microfluidic chip binding kinetics |
| url | https://www.mdpi.com/2079-6374/12/10/785 |
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