Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats
Background: Bone fragility and an increase in susceptibility to fracture osteoporosis is characterized by a reduction in bone mass and the micro-architectural deterioration of bone tissue. There is no previous study regarding the effect of Cinnamomum burmanini Blume on osteoporosis. Objective: This...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947616302571 |
| _version_ | 1811309388410912768 |
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| author | Nia Kania Wahyu Widowati Firli Rahmah Primula Dewi Antonius Christianto Bambang Setiawan Nicolaas Budhiparama Zairin Noor |
| author_facet | Nia Kania Wahyu Widowati Firli Rahmah Primula Dewi Antonius Christianto Bambang Setiawan Nicolaas Budhiparama Zairin Noor |
| author_sort | Nia Kania |
| collection | DOAJ |
| description | Background: Bone fragility and an increase in susceptibility to fracture osteoporosis is characterized by a reduction in bone mass and the micro-architectural deterioration of bone tissue. There is no previous study regarding the effect of Cinnamomum burmanini Blume on osteoporosis. Objective: This study was aimed to investigate the effect of C. burmanini Blume on bone turnover marker, mineral elements, and mesostructure of ovariectomized rats. Materials and methods: Thirty female Wistar rats were randomly divided into five groups, which included a control group (sham surgery), ovariectomy group (OVX), and ovariectomy groups in the presence of ethanolic extract of C. burmanini Blume (EECB) at doses of 12.5; 25; 50 mg/kg body weight (BW). Analysis of serum C-telopeptide collagen type I (CTX) and osteocalcin (OC) were done by enzyme-linked immunosorbent assay (ELISA). Tibia mineral elements and mesostructure were analyzed by X-ray Fluorescence and Scanning Electron Microscopy, respectively. In silico study was performed by modeling protein structure using SWISS-MODEL server and Ramachandran plot analysis. Results: The increase in OC and CTX were significantly attenuated by treatments of EECB. Ovariectomy significantly decreased Cu/Zn ratio compared to sham-operated rats (p < 0.05). Mesostructure of ovariectomized rats was significantly different compared with the control group. Conclusion: Cinnamon was able to normalize bone turnover markers, but, the mesostructure of hydroxyapatite crystal growth was achieved at the highest dose extract. In silico study showed that the active compound of EECB could not only support osteoclastogenesis process by decreasing the binding energy between RANKL and RANK, but also by inhibiting the interaction between OPG and RANK. Keywords: Cinnamon, Bone turnover, Bone mesostructure, Osteoporosis |
| first_indexed | 2024-04-13T09:41:16Z |
| format | Article |
| id | doaj.art-c837c81238724ab599e6ea9868c87c26 |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-04-13T09:41:16Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-c837c81238724ab599e6ea9868c87c262022-12-22T02:51:55ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762018-01-01912026Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized ratsNia Kania0Wahyu Widowati1Firli Rahmah Primula Dewi2Antonius Christianto3Bambang Setiawan4Nicolaas Budhiparama5Zairin Noor6Research Center for Osteoporosis, Department of Pathology, Ulin General Hospital, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia; Corresponding author.Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, IndonesiaMalang In Silico Club, Malang, East Java, IndonesiaMalang In Silico Club, Malang, East Java, IndonesiaResearch Center for Toxicology, Cancer, and Regenerative Medicine, Department of Medical Chemistry and Biochemistry, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, IndonesiaBudhiparama Institute of Hip and Knee Research and Education Foundation for Arthroplasty, Sports Medicine and Osteoporosis, Jakarta, IndonesiaResearch Center for Osteoporosis, Department of Orthopedics and Traumatology, Ulin General Hospital, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, IndonesiaBackground: Bone fragility and an increase in susceptibility to fracture osteoporosis is characterized by a reduction in bone mass and the micro-architectural deterioration of bone tissue. There is no previous study regarding the effect of Cinnamomum burmanini Blume on osteoporosis. Objective: This study was aimed to investigate the effect of C. burmanini Blume on bone turnover marker, mineral elements, and mesostructure of ovariectomized rats. Materials and methods: Thirty female Wistar rats were randomly divided into five groups, which included a control group (sham surgery), ovariectomy group (OVX), and ovariectomy groups in the presence of ethanolic extract of C. burmanini Blume (EECB) at doses of 12.5; 25; 50 mg/kg body weight (BW). Analysis of serum C-telopeptide collagen type I (CTX) and osteocalcin (OC) were done by enzyme-linked immunosorbent assay (ELISA). Tibia mineral elements and mesostructure were analyzed by X-ray Fluorescence and Scanning Electron Microscopy, respectively. In silico study was performed by modeling protein structure using SWISS-MODEL server and Ramachandran plot analysis. Results: The increase in OC and CTX were significantly attenuated by treatments of EECB. Ovariectomy significantly decreased Cu/Zn ratio compared to sham-operated rats (p < 0.05). Mesostructure of ovariectomized rats was significantly different compared with the control group. Conclusion: Cinnamon was able to normalize bone turnover markers, but, the mesostructure of hydroxyapatite crystal growth was achieved at the highest dose extract. In silico study showed that the active compound of EECB could not only support osteoclastogenesis process by decreasing the binding energy between RANKL and RANK, but also by inhibiting the interaction between OPG and RANK. Keywords: Cinnamon, Bone turnover, Bone mesostructure, Osteoporosishttp://www.sciencedirect.com/science/article/pii/S0975947616302571 |
| spellingShingle | Nia Kania Wahyu Widowati Firli Rahmah Primula Dewi Antonius Christianto Bambang Setiawan Nicolaas Budhiparama Zairin Noor Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats Journal of Ayurveda and Integrative Medicine |
| title | Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats |
| title_full | Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats |
| title_fullStr | Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats |
| title_full_unstemmed | Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats |
| title_short | Cinnamomum burmanini Blume increases bone turnover marker and induces tibia's granule formation in ovariectomized rats |
| title_sort | cinnamomum burmanini blume increases bone turnover marker and induces tibia s granule formation in ovariectomized rats |
| url | http://www.sciencedirect.com/science/article/pii/S0975947616302571 |
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