MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide
MicroRNAs (miRNAs), a class of small non-coding RNAs, can induce mRNA degradation or repress translation by binding to the 3′-untranslated region (UTR) of its target mRNA. Recently, some specific miRNAs, e.g. miR-93, have been found to be involved in pathological processes by targeting some oncogene...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2016-06-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/5/6/669 |
| _version_ | 1818941339466727424 |
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| author | Rui Chen Huan Liu Quan Cheng Bing Jiang Renjun Peng Qin Zou Wenren Yang Xiaosheng Yang Xiaobing Wu Zigui Chen |
| author_facet | Rui Chen Huan Liu Quan Cheng Bing Jiang Renjun Peng Qin Zou Wenren Yang Xiaosheng Yang Xiaobing Wu Zigui Chen |
| author_sort | Rui Chen |
| collection | DOAJ |
| description | MicroRNAs (miRNAs), a class of small non-coding RNAs, can induce mRNA degradation or repress translation by binding to the 3′-untranslated region (UTR) of its target mRNA. Recently, some specific miRNAs, e.g. miR-93, have been found to be involved in pathological processes by targeting some oncogenes or tumor suppressors in glioma. However, the regulatory mechanism of miR-93 in the biological behaviors and chemoresistance of glioma cells remains unclear. In the present study, in situ hybridization and real-time RT-PCR data indicated that miR-93 was significantly upregulated in glioma patients (n=43) compared with normal brain tissues (n=8). Moreover, the upregulated miR-93 level was significantly associated with the advanced malignancy. We also found that upregulation of miR-93 promoted the proliferation, migration and invasion of glioma cells, and that miR-93 was involved in the regulation of cell cycle progression by mediating the protein levels of P21, P27, P53 and Cyclin D1. P21 was further identified as a direct target of miR-93. Knockdown of P21 attenuated the suppressive effects of miR-93 inhibition on cell cycle progression and colony formation. In addition, inhibition of miR-93 enhanced the chemosensitization of glioma cells to temozolomide (TMZ). Based on these above data, our study demonstrates that miR-93, upregulated in glioma, promotes the proliferation, cell cycle progression, migration and invasion of human glioma cells and suppresses their chemosensitivity to TMZ. Therefore, miR-93 may become a promising diagnostic marker and therapeutic target for glioma. |
| first_indexed | 2024-12-20T06:53:58Z |
| format | Article |
| id | doaj.art-c8387c194ad642b9ab25eb588cc5bcc0 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-20T06:53:58Z |
| publishDate | 2016-06-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-c8387c194ad642b9ab25eb588cc5bcc02022-12-21T19:49:25ZengThe Company of BiologistsBiology Open2046-63902016-06-015666967710.1242/bio.015552015552MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomideRui Chen0Huan Liu1Quan Cheng2Bing Jiang3Renjun Peng4Qin Zou5Wenren Yang6Xiaosheng Yang7Xiaobing Wu8Zigui Chen9 Department of Neurosurgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Cardiology, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China Department of Neurosurgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Neurosurgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Neurosurgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Neurosurgery, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421001, China Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China MicroRNAs (miRNAs), a class of small non-coding RNAs, can induce mRNA degradation or repress translation by binding to the 3′-untranslated region (UTR) of its target mRNA. Recently, some specific miRNAs, e.g. miR-93, have been found to be involved in pathological processes by targeting some oncogenes or tumor suppressors in glioma. However, the regulatory mechanism of miR-93 in the biological behaviors and chemoresistance of glioma cells remains unclear. In the present study, in situ hybridization and real-time RT-PCR data indicated that miR-93 was significantly upregulated in glioma patients (n=43) compared with normal brain tissues (n=8). Moreover, the upregulated miR-93 level was significantly associated with the advanced malignancy. We also found that upregulation of miR-93 promoted the proliferation, migration and invasion of glioma cells, and that miR-93 was involved in the regulation of cell cycle progression by mediating the protein levels of P21, P27, P53 and Cyclin D1. P21 was further identified as a direct target of miR-93. Knockdown of P21 attenuated the suppressive effects of miR-93 inhibition on cell cycle progression and colony formation. In addition, inhibition of miR-93 enhanced the chemosensitization of glioma cells to temozolomide (TMZ). Based on these above data, our study demonstrates that miR-93, upregulated in glioma, promotes the proliferation, cell cycle progression, migration and invasion of human glioma cells and suppresses their chemosensitivity to TMZ. Therefore, miR-93 may become a promising diagnostic marker and therapeutic target for glioma.http://bio.biologists.org/content/5/6/669GliomaMicroRNAProliferationInvasionCell cycleTemozolomide |
| spellingShingle | Rui Chen Huan Liu Quan Cheng Bing Jiang Renjun Peng Qin Zou Wenren Yang Xiaosheng Yang Xiaobing Wu Zigui Chen MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide Biology Open Glioma MicroRNA Proliferation Invasion Cell cycle Temozolomide |
| title | MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| title_full | MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| title_fullStr | MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| title_full_unstemmed | MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| title_short | MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| title_sort | microrna 93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide |
| topic | Glioma MicroRNA Proliferation Invasion Cell cycle Temozolomide |
| url | http://bio.biologists.org/content/5/6/669 |
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