The cell proliferation antigen Ki-67 organises heterochromatin
Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/13722 |
| _version_ | 1811180638071422976 |
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| author | Michal Sobecki Karim Mrouj Alain Camasses Nikolaos Parisis Emilien Nicolas David Llères François Gerbe Susana Prieto Liliana Krasinska Alexandre David Manuel Eguren Marie-Christine Birling Serge Urbach Sonia Hem Jérôme Déjardin Marcos Malumbres Philippe Jay Vjekoslav Dulic Denis LJ Lafontaine Robert Feil Daniel Fisher |
| author_facet | Michal Sobecki Karim Mrouj Alain Camasses Nikolaos Parisis Emilien Nicolas David Llères François Gerbe Susana Prieto Liliana Krasinska Alexandre David Manuel Eguren Marie-Christine Birling Serge Urbach Sonia Hem Jérôme Déjardin Marcos Malumbres Philippe Jay Vjekoslav Dulic Denis LJ Lafontaine Robert Feil Daniel Fisher |
| author_sort | Michal Sobecki |
| collection | DOAJ |
| description | Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not prevent cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 was relocalised within the nucleus. Finally, overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression. |
| first_indexed | 2024-04-11T09:06:32Z |
| format | Article |
| id | doaj.art-c83b0fd6e4ff45c3b6dae215afe87785 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:32Z |
| publishDate | 2016-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c83b0fd6e4ff45c3b6dae215afe877852022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.13722The cell proliferation antigen Ki-67 organises heterochromatinMichal Sobecki0Karim Mrouj1Alain Camasses2Nikolaos Parisis3https://orcid.org/0000-0002-5706-0122Emilien Nicolas4David Llères5François Gerbe6Susana Prieto7Liliana Krasinska8Alexandre David9Manuel Eguren10https://orcid.org/0000-0003-0850-939XMarie-Christine Birling11Serge Urbach12Sonia Hem13Jérôme Déjardin14Marcos Malumbres15https://orcid.org/0000-0002-0829-6315Philippe Jay16Vjekoslav Dulic17Denis LJ Lafontaine18Robert Feil19https://orcid.org/0000-0002-5671-5860Daniel Fisher20https://orcid.org/0000-0002-0822-3482Montpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceRNA Molecular Biology, Center for Microscopy and Molecular Imaging, Fonds de la Recherche Nationale, Université Libre de Bruxelles, Charleroi-Gosselies, BelgiumMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceFaculty of Sciences, University of Montpellier, Montpellier, France; Institute of Functional Genomics (IGF), CNRS UMR 5203, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; U1191, Inserm, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceFaculty of Sciences, University of Montpellier, Montpellier, France; Institute of Functional Genomics (IGF), CNRS UMR 5203, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; U1191, Inserm, Montpellier, FranceSpanish National Cancer Research Centre, Madrid, SpainICS, Mouse Clinical Institute, Illkirch-Graffenstaden, FranceFaculty of Sciences, University of Montpellier, Montpellier, France; Institute of Functional Genomics (IGF), CNRS UMR 5203, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; U1191, Inserm, Montpellier, France; Functional Proteomics Platform, Institute of Functional Genomics, Montpellier, FranceMass Spectrometry Platform MSPP, SupAgro, Montpellier, FranceFaculty of Sciences, University of Montpellier, Montpellier, France; Institute of Human Genetics (IGH) CNRS UPR 1142, Centre National de la Recherche Scientifique, Montpellier, FranceSpanish National Cancer Research Centre, Madrid, SpainFaculty of Sciences, University of Montpellier, Montpellier, France; Institute of Functional Genomics (IGF), CNRS UMR 5203, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; U1191, Inserm, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceRNA Molecular Biology, Center for Microscopy and Molecular Imaging, Fonds de la Recherche Nationale, Université Libre de Bruxelles, Charleroi-Gosselies, BelgiumMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceMontpellier Institute of Molecular Genetics (IGMM) CNRS UMR 5535, Centre National de la Recherche Scientifique (CNRS), Montpellier, France; Faculty of Sciences, University of Montpellier, Montpellier, FranceAntigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not prevent cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 was relocalised within the nucleus. Finally, overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression.https://elifesciences.org/articles/13722Ki-67heterochromatincell proliferation |
| spellingShingle | Michal Sobecki Karim Mrouj Alain Camasses Nikolaos Parisis Emilien Nicolas David Llères François Gerbe Susana Prieto Liliana Krasinska Alexandre David Manuel Eguren Marie-Christine Birling Serge Urbach Sonia Hem Jérôme Déjardin Marcos Malumbres Philippe Jay Vjekoslav Dulic Denis LJ Lafontaine Robert Feil Daniel Fisher The cell proliferation antigen Ki-67 organises heterochromatin eLife Ki-67 heterochromatin cell proliferation |
| title | The cell proliferation antigen Ki-67 organises heterochromatin |
| title_full | The cell proliferation antigen Ki-67 organises heterochromatin |
| title_fullStr | The cell proliferation antigen Ki-67 organises heterochromatin |
| title_full_unstemmed | The cell proliferation antigen Ki-67 organises heterochromatin |
| title_short | The cell proliferation antigen Ki-67 organises heterochromatin |
| title_sort | cell proliferation antigen ki 67 organises heterochromatin |
| topic | Ki-67 heterochromatin cell proliferation |
| url | https://elifesciences.org/articles/13722 |
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