Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis
Background/Aims: Bone marrow-derived cells (BMDCs) reduced mechanical and histologic changes in the lung in a murine model of silicosis, but these beneficial effects did not persist in the course of lung injury. We hypothesized that repeated administration of BMDCs may decrease lung inflammation and...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2013-12-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/356603 |
| _version_ | 1818142543808823296 |
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| author | Miquéias Lopes-Pacheco Debora G. Xisto Felipe M. Ornellas Mariana A. Antunes Soraia C. Abreu Patricia R.M. Rocco Christina M. Takiya Marcelo M. Morales |
| author_facet | Miquéias Lopes-Pacheco Debora G. Xisto Felipe M. Ornellas Mariana A. Antunes Soraia C. Abreu Patricia R.M. Rocco Christina M. Takiya Marcelo M. Morales |
| author_sort | Miquéias Lopes-Pacheco |
| collection | DOAJ |
| description | Background/Aims: Bone marrow-derived cells (BMDCs) reduced mechanical and histologic changes in the lung in a murine model of silicosis, but these beneficial effects did not persist in the course of lung injury. We hypothesized that repeated administration of BMDCs may decrease lung inflammation and remodeling thus preventing disease progression. Methods: One hundred and two C57BL/6 mice were randomly divided into SIL (silica, 20 mg intratracheally [IT]) and control (C) groups (saline, IT). C and SIL groups were further randomized to receive BMDCs (2×106 cells) or saline IT 15 and 30 days after the start of the protocol. Results: By day 60, BMDCs had decreased the fractional area of granuloma and the number of polymorphonuclear cells, macrophages (total and M1 phenotype), apoptotic cells, the level of transforming growth factor (TGF)-β‚ and types I and III collagen fiber content in the granuloma. In the alveolar septa, BMDCs reduced the amount of collagen and elastic fibers, TGF-β, and the number of M1 and apoptotic cells. Furthermore, interleukin (IL)-1β, IL-1R1, caspase-3 mRNA levels decreased and the level of IL-1RN mRNA increased. Lung mechanics improved after BMDC therapy. The presence of male donor cells in lung tissue was not observed using detection of Y chromosome DNA. Conclusion: repeated administration of BMDCs reduced inflammation, fibrogenesis, and elastogenesis, thus improving lung mechanics through the release of paracrine factors. |
| first_indexed | 2024-12-11T11:17:27Z |
| format | Article |
| id | doaj.art-c83b151f45824144b4765bd2d69f9f60 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-11T11:17:27Z |
| publishDate | 2013-12-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-c83b151f45824144b4765bd2d69f9f602022-12-22T01:09:17ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-12-013261681169410.1159/000356603356603Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental SilicosisMiquéias Lopes-PachecoDebora G. XistoFelipe M. OrnellasMariana A. AntunesSoraia C. AbreuPatricia R.M. RoccoChristina M. TakiyaMarcelo M. MoralesBackground/Aims: Bone marrow-derived cells (BMDCs) reduced mechanical and histologic changes in the lung in a murine model of silicosis, but these beneficial effects did not persist in the course of lung injury. We hypothesized that repeated administration of BMDCs may decrease lung inflammation and remodeling thus preventing disease progression. Methods: One hundred and two C57BL/6 mice were randomly divided into SIL (silica, 20 mg intratracheally [IT]) and control (C) groups (saline, IT). C and SIL groups were further randomized to receive BMDCs (2×106 cells) or saline IT 15 and 30 days after the start of the protocol. Results: By day 60, BMDCs had decreased the fractional area of granuloma and the number of polymorphonuclear cells, macrophages (total and M1 phenotype), apoptotic cells, the level of transforming growth factor (TGF)-β‚ and types I and III collagen fiber content in the granuloma. In the alveolar septa, BMDCs reduced the amount of collagen and elastic fibers, TGF-β, and the number of M1 and apoptotic cells. Furthermore, interleukin (IL)-1β, IL-1R1, caspase-3 mRNA levels decreased and the level of IL-1RN mRNA increased. Lung mechanics improved after BMDC therapy. The presence of male donor cells in lung tissue was not observed using detection of Y chromosome DNA. Conclusion: repeated administration of BMDCs reduced inflammation, fibrogenesis, and elastogenesis, thus improving lung mechanics through the release of paracrine factors.http://www.karger.com/Article/FullText/356603Cell therapyInflammationFibrosisElastic fiberSilicosis |
| spellingShingle | Miquéias Lopes-Pacheco Debora G. Xisto Felipe M. Ornellas Mariana A. Antunes Soraia C. Abreu Patricia R.M. Rocco Christina M. Takiya Marcelo M. Morales Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis Cellular Physiology and Biochemistry Cell therapy Inflammation Fibrosis Elastic fiber Silicosis |
| title | Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis |
| title_full | Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis |
| title_fullStr | Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis |
| title_full_unstemmed | Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis |
| title_short | Repeated Administration of Bone Marrow-Derived Cells Prevents Disease Progression in Experimental Silicosis |
| title_sort | repeated administration of bone marrow derived cells prevents disease progression in experimental silicosis |
| topic | Cell therapy Inflammation Fibrosis Elastic fiber Silicosis |
| url | http://www.karger.com/Article/FullText/356603 |
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