Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection
Tomato chlorosis virus (ToCV) severely threatens tomato production worldwide. P27 is known to be involved in virion assembly, but its other roles in ToCV infection are unclear. In this study, we found that removal of p27 reduced systemic infection, while ectopic expression of p27 promoted systemic i...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/4/990 |
| _version_ | 1797603241664446464 |
|---|---|
| author | Xiaohui Sun Lianyi Zang Xiaoying Liu Shanshan Jiang Xianping Zhang Dan Zhao Kaijie Shang Tao Zhou Changxiang Zhu Xiaoping Zhu |
| author_facet | Xiaohui Sun Lianyi Zang Xiaoying Liu Shanshan Jiang Xianping Zhang Dan Zhao Kaijie Shang Tao Zhou Changxiang Zhu Xiaoping Zhu |
| author_sort | Xiaohui Sun |
| collection | DOAJ |
| description | Tomato chlorosis virus (ToCV) severely threatens tomato production worldwide. P27 is known to be involved in virion assembly, but its other roles in ToCV infection are unclear. In this study, we found that removal of p27 reduced systemic infection, while ectopic expression of p27 promoted systemic infection of potato virus X in <i>Nicotiana benthamiana</i>. We determined that <i>Solanum lycopersicum</i> catalases (SlCAT) can interact with p27 in vitro and in vivo and that amino acids 73 to 77 of the N-terminus of SlCAT represent the critical region for their interaction. p27 is distributed in the cytoplasm and nucleus, and its coexpression with SlCAT1 or SlCAT2 changes its distribution in the nucleus. Furthermore, we found that silencing of <i>SlCAT1</i> and <i>SlCAT2</i> can promote ToCV infection. In conclusion, p27 can promote viral infection by binding directly to inhibit anti-ToCV processes mediated by SlCAT1 or SlCAT2. |
| first_indexed | 2024-03-11T04:26:31Z |
| format | Article |
| id | doaj.art-c83d3f39e5014fcbbc4f55731d2388fc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T04:26:31Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c83d3f39e5014fcbbc4f55731d2388fc2023-11-17T21:46:38ZengMDPI AGViruses1999-49152023-04-0115499010.3390/v15040990Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral InfectionXiaohui Sun0Lianyi Zang1Xiaoying Liu2Shanshan Jiang3Xianping Zhang4Dan Zhao5Kaijie Shang6Tao Zhou7Changxiang Zhu8Xiaoping Zhu9Shandong Province Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCollege of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, ChinaCollege of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, ChinaShandong Province Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCollege of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, ChinaCollege of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, ChinaCollege of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Agrobiotechnology, Department of Plant Pathology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaShandong Province Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaTomato chlorosis virus (ToCV) severely threatens tomato production worldwide. P27 is known to be involved in virion assembly, but its other roles in ToCV infection are unclear. In this study, we found that removal of p27 reduced systemic infection, while ectopic expression of p27 promoted systemic infection of potato virus X in <i>Nicotiana benthamiana</i>. We determined that <i>Solanum lycopersicum</i> catalases (SlCAT) can interact with p27 in vitro and in vivo and that amino acids 73 to 77 of the N-terminus of SlCAT represent the critical region for their interaction. p27 is distributed in the cytoplasm and nucleus, and its coexpression with SlCAT1 or SlCAT2 changes its distribution in the nucleus. Furthermore, we found that silencing of <i>SlCAT1</i> and <i>SlCAT2</i> can promote ToCV infection. In conclusion, p27 can promote viral infection by binding directly to inhibit anti-ToCV processes mediated by SlCAT1 or SlCAT2.https://www.mdpi.com/1999-4915/15/4/990tomato chlorosis virusp27catalasevirus host proteins |
| spellingShingle | Xiaohui Sun Lianyi Zang Xiaoying Liu Shanshan Jiang Xianping Zhang Dan Zhao Kaijie Shang Tao Zhou Changxiang Zhu Xiaoping Zhu Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection Viruses tomato chlorosis virus p27 catalase virus host proteins |
| title | Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection |
| title_full | Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection |
| title_fullStr | Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection |
| title_full_unstemmed | Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection |
| title_short | Interactions of Tomato Chlorosis Virus p27 Protein with Tomato Catalase Are Involved in Viral Infection |
| title_sort | interactions of tomato chlorosis virus p27 protein with tomato catalase are involved in viral infection |
| topic | tomato chlorosis virus p27 catalase virus host proteins |
| url | https://www.mdpi.com/1999-4915/15/4/990 |
| work_keys_str_mv | AT xiaohuisun interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT lianyizang interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT xiaoyingliu interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT shanshanjiang interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT xianpingzhang interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT danzhao interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT kaijieshang interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT taozhou interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT changxiangzhu interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection AT xiaopingzhu interactionsoftomatochlorosisvirusp27proteinwithtomatocatalaseareinvolvedinviralinfection |