The possible role of Myosin light chain in myoblast proliferation
Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-01-01
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| Series: | Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013 |
| _version_ | 1818274921728442368 |
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| author | SU-ZHEN ZHANG YONG XU HUI-QI XIE XIU-QUN LI YU-QUAN WEI ZHI-MING YANG |
| author_facet | SU-ZHEN ZHANG YONG XU HUI-QI XIE XIU-QUN LI YU-QUAN WEI ZHI-MING YANG |
| author_sort | SU-ZHEN ZHANG |
| collection | DOAJ |
| description | Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast proliferation and delay the differentiation of myoblasts. Our results suggested that Myl, likely Myll, can negatively affect myoblast proliferation by facilitating myoblast withdrawal from cell cycle and differentiation. |
| first_indexed | 2024-12-12T22:21:32Z |
| format | Article |
| id | doaj.art-c8409e97226544a6b174cda2d04709d8 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-12T22:21:32Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-c8409e97226544a6b174cda2d04709d82022-12-22T00:09:54ZengBMCBiological Research0716-97600717-62872009-01-01421121132The possible role of Myosin light chain in myoblast proliferationSU-ZHEN ZHANGYONG XUHUI-QI XIEXIU-QUN LIYU-QUAN WEIZHI-MING YANGSkeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast proliferation and delay the differentiation of myoblasts. Our results suggested that Myl, likely Myll, can negatively affect myoblast proliferation by facilitating myoblast withdrawal from cell cycle and differentiation.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013Myosin light chainProliferationMyoblastExtraocular muscle |
| spellingShingle | SU-ZHEN ZHANG YONG XU HUI-QI XIE XIU-QUN LI YU-QUAN WEI ZHI-MING YANG The possible role of Myosin light chain in myoblast proliferation Biological Research Myosin light chain Proliferation Myoblast Extraocular muscle |
| title | The possible role of Myosin light chain in myoblast proliferation |
| title_full | The possible role of Myosin light chain in myoblast proliferation |
| title_fullStr | The possible role of Myosin light chain in myoblast proliferation |
| title_full_unstemmed | The possible role of Myosin light chain in myoblast proliferation |
| title_short | The possible role of Myosin light chain in myoblast proliferation |
| title_sort | possible role of myosin light chain in myoblast proliferation |
| topic | Myosin light chain Proliferation Myoblast Extraocular muscle |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013 |
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