Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria
Genetic variants at heat shock protein 70 gene and their influence on heat stress (HS) tolerance were studied among selected Nigeria zebu, namely, 25 White Fulani (WF), 21 Sokoto Gudali (SG), 21 Red Bororo (RB), and 23 Ambala (AM). Detection of single nucleotide polymorphism (SNP) followed by determ...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.642213/full |
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| author | Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya George Mutani Msalya Aranganoor Kannan Thiruvenkadan Chirukandoth Sreekumar Gopalan Krishnaswamy Tirumurugaan Adeboye O. Fafiolu Matthew A. Adeleke Abdulmojeed Yakubu Christian Obiora Ndubuisi Ikeobi Moses Okpeku |
| author_facet | Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya George Mutani Msalya Aranganoor Kannan Thiruvenkadan Chirukandoth Sreekumar Gopalan Krishnaswamy Tirumurugaan Adeboye O. Fafiolu Matthew A. Adeleke Abdulmojeed Yakubu Christian Obiora Ndubuisi Ikeobi Moses Okpeku |
| author_sort | Gbolabo Olaitan Onasanya |
| collection | DOAJ |
| description | Genetic variants at heat shock protein 70 gene and their influence on heat stress (HS) tolerance were studied among selected Nigeria zebu, namely, 25 White Fulani (WF), 21 Sokoto Gudali (SG), 21 Red Bororo (RB), and 23 Ambala (AM). Detection of single nucleotide polymorphism (SNP) followed by determination of genotype and genotypic frequency was made among the selected breeds. The heat tolerance coefficient (HTC) was determined from thermo-related parameters including body temperature, rectal temperature, and respiratory rate. Thermo-Tolerance was evaluated through the SNP–thermo-parameter relationship. Statistical analyses were done using the GLM procedure in SAS. A quantitative real-time/high-resolution melting-based assay detected twelve genetic variants. Five of these were common and shared across all breeds of cattle. Of the remaining seven variants, three were specifically identified in AM, two in SG, and two in RB. Also, SNPs were evaluated and four unique SNPs (C151T, C146T, G90A, and C219A) were identified. Heterozygous animals had lower HTC suggesting their potential to withstand HS than homozygous counterparts. The WF and RB animals had significantly lower values for all parameters (BT, RT, RR, and HTC) compared to AM and SG breeds. Thermo-related parameters were significantly different (P < 0.001), and it is recommended that screening of SNPs in zebu is needed to enable selection for improved thermo-tolerance. |
| first_indexed | 2024-12-17T02:28:39Z |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T02:28:39Z |
| publishDate | 2021-04-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-c842bda2111448e5bbf66420a137edfe2022-12-21T22:07:02ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-04-011210.3389/fgene.2021.642213642213Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of NigeriaGbolabo Olaitan Onasanya0Gbolabo Olaitan Onasanya1Gbolabo Olaitan Onasanya2Gbolabo Olaitan Onasanya3George Mutani Msalya4Aranganoor Kannan Thiruvenkadan5Chirukandoth Sreekumar6Gopalan Krishnaswamy Tirumurugaan7Adeboye O. Fafiolu8Matthew A. Adeleke9Abdulmojeed Yakubu10Christian Obiora Ndubuisi Ikeobi11Moses Okpeku12Department of Animal Science, Federal University Dutse, Dutse, NigeriaDepartment of Animal Breeding and Genetics, Federal University of Agriculture Abeokuta, Abeokuta, NigeriaMecheri Sheep Research Station Pottaneri, Tamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaBiotechnology Laboratory, Post Graduate Research Institute in Animal Sciences, Tamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaDepartment of Animal, Aquaculture and Range Sciences, Sokoine University of Agriculture, Morogoro, TanzaniaMecheri Sheep Research Station Pottaneri, Tamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaBiotechnology Laboratory, Post Graduate Research Institute in Animal Sciences, Tamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaDepartment of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaDepartment of Animal Nutrition, Federal University of Agriculture, Abeokuta, Abeokuta, NigeriaDiscipline of Genetics, School of Life Sciences, College of Agriculture, Engineering and Sciences, University of KwaZulu-Natal, Durban, South AfricaDepartment of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, NigeriaDepartment of Animal Breeding and Genetics, Federal University of Agriculture Abeokuta, Abeokuta, NigeriaDiscipline of Genetics, School of Life Sciences, College of Agriculture, Engineering and Sciences, University of KwaZulu-Natal, Durban, South AfricaGenetic variants at heat shock protein 70 gene and their influence on heat stress (HS) tolerance were studied among selected Nigeria zebu, namely, 25 White Fulani (WF), 21 Sokoto Gudali (SG), 21 Red Bororo (RB), and 23 Ambala (AM). Detection of single nucleotide polymorphism (SNP) followed by determination of genotype and genotypic frequency was made among the selected breeds. The heat tolerance coefficient (HTC) was determined from thermo-related parameters including body temperature, rectal temperature, and respiratory rate. Thermo-Tolerance was evaluated through the SNP–thermo-parameter relationship. Statistical analyses were done using the GLM procedure in SAS. A quantitative real-time/high-resolution melting-based assay detected twelve genetic variants. Five of these were common and shared across all breeds of cattle. Of the remaining seven variants, three were specifically identified in AM, two in SG, and two in RB. Also, SNPs were evaluated and four unique SNPs (C151T, C146T, G90A, and C219A) were identified. Heterozygous animals had lower HTC suggesting their potential to withstand HS than homozygous counterparts. The WF and RB animals had significantly lower values for all parameters (BT, RT, RR, and HTC) compared to AM and SG breeds. Thermo-related parameters were significantly different (P < 0.001), and it is recommended that screening of SNPs in zebu is needed to enable selection for improved thermo-tolerance.https://www.frontiersin.org/articles/10.3389/fgene.2021.642213/fullheat toleranceheterozygositynative cattlepolymorphismsthermal stress |
| spellingShingle | Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya Gbolabo Olaitan Onasanya George Mutani Msalya Aranganoor Kannan Thiruvenkadan Chirukandoth Sreekumar Gopalan Krishnaswamy Tirumurugaan Adeboye O. Fafiolu Matthew A. Adeleke Abdulmojeed Yakubu Christian Obiora Ndubuisi Ikeobi Moses Okpeku Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria Frontiers in Genetics heat tolerance heterozygosity native cattle polymorphisms thermal stress |
| title | Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria |
| title_full | Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria |
| title_fullStr | Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria |
| title_full_unstemmed | Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria |
| title_short | Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria |
| title_sort | heterozygous single nucleotide polymorphism genotypes at heat shock protein 70 gene potentially influence thermo tolerance among four zebu breeds of nigeria |
| topic | heat tolerance heterozygosity native cattle polymorphisms thermal stress |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.642213/full |
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