Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells
The gastric pathogen <i>Helicobacter pylori</i> infects half of the world’s population and is a major risk factor for gastric cancer development. In order to attach to human gastric epithelial cells and inject the oncoprotein CagA into host cells, <i>H. pylori</i> utilizes th...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1748 |
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| author | Karin Taxauer Youssef Hamway Anna Ralser Alisa Dietl Karin Mink Michael Vieth Bernhard B. Singer Markus Gerhard Raquel Mejías-Luque |
| author_facet | Karin Taxauer Youssef Hamway Anna Ralser Alisa Dietl Karin Mink Michael Vieth Bernhard B. Singer Markus Gerhard Raquel Mejías-Luque |
| author_sort | Karin Taxauer |
| collection | DOAJ |
| description | The gastric pathogen <i>Helicobacter pylori</i> infects half of the world’s population and is a major risk factor for gastric cancer development. In order to attach to human gastric epithelial cells and inject the oncoprotein CagA into host cells, <i>H. pylori</i> utilizes the outer membrane protein HopQ that binds to the cell surface protein CEACAM, which can be expressed on the gastric mucosa. Once bound, <i>H. pylori</i> activates a number of signaling pathways, including canonical and non-canonical NF-κB. We investigated whether HopQ–CEACAM interaction is involved in activating the non-canonical NF-κB signaling pathway. Different gastric cancer cells were infected with the <i>H. pylori</i> wild type, or HopQ mutant strains, and the activation of non-canonical NF-κB was related to CEACAM expression levels. The correlation between CEACAM levels and the activation of non-canonical NF-κB was confirmed in human gastric tissue samples. Taken together, our findings show that the HopQ–CEACAM interaction is important for activation of the non-canonical NF-κB pathway in gastric epithelial cells. |
| first_indexed | 2024-03-10T08:34:33Z |
| format | Article |
| id | doaj.art-c84561a48e9a48209bfb2b551822edd8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:33Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c84561a48e9a48209bfb2b551822edd82023-11-22T08:48:17ZengMDPI AGMicroorganisms2076-26072021-08-0198174810.3390/microorganisms9081748Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial CellsKarin Taxauer0Youssef Hamway1Anna Ralser2Alisa Dietl3Karin Mink4Michael Vieth5Bernhard B. Singer6Markus Gerhard7Raquel Mejías-Luque8Institute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Klinikum Bayreuth, 95445 Bayreuth, GermanyInstitute of Anatomy, Medical Faculty, University of Duisburg-Essen, 45147 Essen, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, 81675 Munich, GermanyThe gastric pathogen <i>Helicobacter pylori</i> infects half of the world’s population and is a major risk factor for gastric cancer development. In order to attach to human gastric epithelial cells and inject the oncoprotein CagA into host cells, <i>H. pylori</i> utilizes the outer membrane protein HopQ that binds to the cell surface protein CEACAM, which can be expressed on the gastric mucosa. Once bound, <i>H. pylori</i> activates a number of signaling pathways, including canonical and non-canonical NF-κB. We investigated whether HopQ–CEACAM interaction is involved in activating the non-canonical NF-κB signaling pathway. Different gastric cancer cells were infected with the <i>H. pylori</i> wild type, or HopQ mutant strains, and the activation of non-canonical NF-κB was related to CEACAM expression levels. The correlation between CEACAM levels and the activation of non-canonical NF-κB was confirmed in human gastric tissue samples. Taken together, our findings show that the HopQ–CEACAM interaction is important for activation of the non-canonical NF-κB pathway in gastric epithelial cells.https://www.mdpi.com/2076-2607/9/8/1748non-canonical NF-ĸBNIKp52<i>H. pylori</i>HopQCEACAM |
| spellingShingle | Karin Taxauer Youssef Hamway Anna Ralser Alisa Dietl Karin Mink Michael Vieth Bernhard B. Singer Markus Gerhard Raquel Mejías-Luque Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells Microorganisms non-canonical NF-ĸB NIK p52 <i>H. pylori</i> HopQ CEACAM |
| title | Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells |
| title_full | Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells |
| title_fullStr | Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells |
| title_full_unstemmed | Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells |
| title_short | Engagement of CEACAM1 by <i>Helicobacter</i> <i>pylori</i> HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells |
| title_sort | engagement of ceacam1 by i helicobacter i i pylori i hopq is important for the activation of non canonical nf κb in gastric epithelial cells |
| topic | non-canonical NF-ĸB NIK p52 <i>H. pylori</i> HopQ CEACAM |
| url | https://www.mdpi.com/2076-2607/9/8/1748 |
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